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The map label for this gene is gadW [H]

Identifier: 209396616

GI number: 209396616

Start: 2097838

End: 2098425

Strand: Reverse

Name: gadW [H]

Synonym: ECH74115_2194

Alternate gene names: 209396616

Gene position: 2098425-2097838 (Counterclockwise)

Preceding gene: 209396008

Following gene: 209397019

Centisome position: 37.66

GC content: 44.05

Gene sequence:

>588_bases
ATGCATTATGGCAAAGTTAAAATTGTCGATATAAGCGAATCCGTCGTAAGTCAATATCTGGAAAGTCAGCATAAGCTGAC
GAGGACTCGTCTGACTGACATTCCGCTTTACCTGTTGCTGGAACCCAACAATCCTGCGTTGGCTGCGGCTTTAATTACCA
GCCAGGGATTTTCCGGAGAGGTCACGGATATGTTTCTTATGATGGCCTGCCTGTCTCTGTTTGAAACAGATGAACGGATG
TCATTGTTTTTAAGTGGATGTTTATCCAGCATAAGTGCCAAAGTCAGGGCGATAATTCAGACAGATATATCAGCAAGCTG
GACGCTTGGTGCGATTGCTCTACAGTTGCATATGAGTGAGAGTTTGTTAAAGACAAAACTGAAAAATGAAGGGGGCATGT
TCAGTCGCTTGTTGCTGGAAGAGCGGATGCGTGTTGCTGTAAATATGTTATGTTCCCGGTATGGATATGGACAGGCTGTA
GCAGAAAAATGTGGTTATTCAAGCCGGTCCTACTTTATTTCTGTATTTCACCGCTATTATGGCTTCCCGCCAGACAGATA
TGTATCCAGGCAAGGGCTTGATTATTGA

Upstream 100 bases:

>100_bases
GGTTTGCTCCCCAATTAATATTTTTCTTGAAAAGGATACGTTGTCACTTAAGCCAGGCTCAGTCGTTCTGGCCACCAAAT
GCATCAGGGAGCTTTTCCTT

Downstream 100 bases:

>100_bases
TTTTCATCTGATTATTATTTTTTGGCTCGGCCCTTTAGCTCAGTGGTGAGAGCGAGCGACTCATAATCGCCAGGTCGCTG
GTTCAAATCCAGCAAGGGCC

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERM
SLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAV
AEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY

Sequences:

>Translated_195_residues
MHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERM
SLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAV
AEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY
>Mature_195_residues
MHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERM
SLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAV
AEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=183, Percent_Identity=36.0655737704918, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787776, Length=193, Percent_Identity=33.160621761658, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI1786776, Length=195, Percent_Identity=30.2564102564103, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786778, Length=138, Percent_Identity=40.5797101449275, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789933, Length=129, Percent_Identity=41.0852713178295, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1790557, Length=193, Percent_Identity=33.160621761658, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 21969; Mature: 21969

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCH
VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYY
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHH
GFPPDRYVSRQGLDY
CCCCHHHHCCCCCCC
>Mature Secondary Structure
MHYGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCH
VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYY
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHH
GFPPDRYVSRQGLDY
CCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]