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The map label for this gene is hyuA [H]

Identifier: 209396584

GI number: 209396584

Start: 3852744

End: 3854141

Strand: Direct

Name: hyuA [H]

Synonym: ECH74115_4163

Alternate gene names: 209396584

Gene position: 3852744-3854141 (Clockwise)

Preceding gene: 209396009

Following gene: 209400118

Centisome position: 69.14

GC content: 53.08

Gene sequence:

>1398_bases
TTGGAGTTTGCTATGCGCGTATTGATCAAAAACGGCACTGTCGTTAACGCAGATGGACAAGCCAAACAGGATTTGCTGAT
TGAAAGCGGGATTGTTCGCCAGTTGGGCAACAATATTTCGCCGCAGCTCCCGTATGAAGAAATTGATGCCACTGGCTGTT
ACGTTTTCCCTGGCGGCGTGGATGTCCATACGCATTTCAATATTGATGTCGGCATCGCGCGCAGTTGTGATGATTTTTTT
ACCGGTACCCGCGCAGCTGCGTGTGGCGGTACAACAACCATTATTGACCATATGGGATTTGGCCCAAACGGCTGTCGGTT
ACGCCATCAACTGGAGGTTTATCGTGGTTATGCCGCCCATAAAGCGGTCATCGATTACAGCTTTCACGGTGTGATCCAGC
ACATTAATCACGCCATCCTCGACGAAATCCCGATGATGGTCGAGGAAGGGCTGAGCAGTTTTAAACTCTATTTAACCTAT
CAATACAAACTCAACGATGACGAGGTTTTGCAGGCATTACGCCGTCTGCATGAATCCGGCGCGCTGACCACCGTGCACCC
GGAAAATGATGCGGCTATCGCCAGCAAGCGGGCGGAATTTATCGCCGCAGGGTTAACCGCGCCGCGCTATCATGCCTTGA
GTCGCCCTCTGGAATGCGAAGCGGAAGCCATCGCCCGCATGATTAACCTGGCACAAATTGCCGGTAACGCCCCGCTCTAT
ATCGTGCACCTGTCTAACGGCTTAGGTCTGGATTATCTGCGTCTTGCCCGTGCGAATCACCAGCCAGTCTGGGTTGAAAC
CTGCCCACAATATCTCCTGTTGGACGAACGCAGTTACGATACAGAAGACGGCATGAAGTTCATTCTTAGCCCACCGCTGC
GTAACGTACGCGAGCAGGACAAACTGTGGTGTGGCATCAGCGATGGTGCGATTGACGTGGTGGCGACCGATCACTGCACC
TTCTCGATGGCTCAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCTTACCCGGTGTGGAAAACCG
CATGCAGTTACTGTTTTCCAGTGGCGTGATGACGGGACGTATAACACCGGAACGCTTTGTTGAATTAACCAGCGCAATGC
CCGCCAGGCTGTTTGGCCTGTGGCCGCAAAAAGGATTATTAGCGCCCGGTTCCGATGGCGACGTGGTGATTATCGACCCA
CGTCAGAGCCAACAAATTCAGCATCGCCATCTCCACGACAACGCCGACTACTCGCCATGGGAGGGTTTTACCTGTCAGGG
CGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTT
TCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAA

Upstream 100 bases:

>100_bases
CTGGTGACCTGTGCCGCGATGTACGCTGCAATCCCGTTAAGCTGGCTGGCAACCGAATAATTACTCTTCACTTATTCCCC
TCCGGTTCGCCGGAGGGTTT

Downstream 100 bases:

>100_bases
CCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGG
AATGCGCTGGGCGATGACCT

Product: phenylhydantoinase

Products: NA

Alternate protein names: Hydantoin-utilizing enzyme hyuA [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MEFAMRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFF
TGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTY
QYKLNDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLY
IVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCT
FSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDP
RQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

Sequences:

>Translated_465_residues
MEFAMRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFF
TGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTY
QYKLNDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLY
IVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCT
FSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDP
RQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
>Mature_465_residues
MEFAMRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHTHFNIDVGIARSCDDFF
TGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTY
QYKLNDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLY
IVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCT
FSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDP
RQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'-position. Has no activity on dihydropyrimidines. The physiological function is unknown [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=471, Percent_Identity=36.5180467091295, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI4503379, Length=462, Percent_Identity=35.0649350649351, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI4503377, Length=462, Percent_Identity=35.4978354978355, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI4503051, Length=462, Percent_Identity=35.4978354978355, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI62422571, Length=462, Percent_Identity=35.4978354978355, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI19923821, Length=464, Percent_Identity=34.2672413793103, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI190194363, Length=462, Percent_Identity=34.1991341991342, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI18105007, Length=405, Percent_Identity=23.7037037037037, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI87082175, Length=461, Percent_Identity=99.7830802603037, Blast_Score=958, Evalue=0.0,
Organism=Escherichia coli, GI1786722, Length=448, Percent_Identity=29.4642857142857, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71989490, Length=467, Percent_Identity=36.4025695931477, Blast_Score=277, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17539558, Length=463, Percent_Identity=35.85313174946, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI86575075, Length=473, Percent_Identity=28.5412262156448, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI193204318, Length=409, Percent_Identity=25.1833740831296, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322218, Length=423, Percent_Identity=27.6595744680851, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI221377917, Length=481, Percent_Identity=35.7588357588358, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI17137462, Length=468, Percent_Identity=35.6837606837607, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24644287, Length=310, Percent_Identity=36.4516129032258, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24644289, Length=235, Percent_Identity=38.7234042553192, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24642586, Length=408, Percent_Identity=25.7352941176471, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859883, Length=461, Percent_Identity=24.5119305856833, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.-.-.- [C]

Molecular weight: Translated: 51522; Mature: 51522

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFAMRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGV
CCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCE
DVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAH
EEEEEEEEEEECCCCCHHHHCCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCHHHH
KAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESG
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHCC
ALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLY
CEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
IVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQD
EEECCCCCCHHHHHHHHCCCCEEEHCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHC
KLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGR
CEEEEECCCEEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEEC
ITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPW
CCHHHHHHHHHCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCC
EGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
CCEEECHHHHHHHCCCCEEEECCEECCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MEFAMRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGV
CCCEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEECCCE
DVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAH
EEEEEEEEEEECCCCCHHHHCCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCHHHH
KAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESG
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHHHHHHCC
ALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLY
CEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
IVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQD
EEECCCCCCHHHHHHHHCCCCEEEHCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHC
KLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGR
CEEEEECCCEEEEEEECCHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEEC
ITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPW
CCHHHHHHHHHCCHHHHHCCCCCCCEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCC
EGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
CCEEECHHHHHHHCCCCEEEECCEECCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA