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The map label for this gene is ycjW [H]

Identifier: 209396470

GI number: 209396470

Start: 1857793

End: 1858791

Strand: Reverse

Name: ycjW [H]

Synonym: ECH74115_1965

Alternate gene names: 209396470

Gene position: 1858791-1857793 (Counterclockwise)

Preceding gene: 209398083

Following gene: 209399768

Centisome position: 33.36

GC content: 45.25

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
CAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAACCAAACAAGCTGGCCCGCG
CGCTGACCTCTTCGGGTTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCGTTTTTC
TCTGAAGTTTTGCATGCCATCACCGCCAAAGCTGAAGAAGAAGGTTTCGACGTGATATTGCAGACGTCGCACACCCCGGC
AGAAGACTTACAAAAATGTGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTAAGTTCGCCAGCGGATG
AGTCATTTTTTGCCCAACTTGATAAATACGATATTCCTGTGGTGGTGATTGGCAAAGTTGAAGGTCAATATGCCCATGTT
TATTCTGTCGATACCGATAATTTTGGTGACAGCATTGCGTTGACCGATGCGTTAATTGAAAGTGGACATCAAAATATTGC
CTGTCTGCATGCACCGCTTGATGTCCATGTTTCAGTGGATCGGGTAAATGGTTATAAGCAGAGTCTGGAAGATCATAATA
TTGCAGTGCGTGATGAATGGATTGTTGATGGCGGTTATACCCATGAAACAGCACTGCAAGCAGCACGGCAATTATTAAGT
CAGTCACCGTTGCCTGAGGCAGTGTTTGCCACTGACAGCCTGAAATTAATGAGCATTTATCGTGCGGCGGCAGAGAAAAA
CATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGCAATGAAACGCTGTCATTTATTTTAACGCCTGCACCGGGCG
GCATCGATGTTCCGACGCAGGAGTTAGGTCGACAAAGCTGTGAGTTATTATTCCGCTTAATTGCCGGAAAACCGTCACCA
CAAAATATTACCGTTGCCACGCATATGACGCTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTACCCGTGTTGTCATCGACGGGCGAACTCCATAAGATGTGCAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATACCAGCGACCTTGCCTGAATGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACATTTATAGATCGGATAAAGTAC
TCGCGCCGCATCCGGCAATT

Product: transcriptional regulator, LacI family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRDEWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSP
QNITVATHMTLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRDEWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSP
QNITVATHMTLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVY
SVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRDEWIVDGGYTHETALQAARQLLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQ
NITVATHMTLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=98.1927710843374, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.8641975308642, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=30.4487179487179, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=28.4829721362229, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=27.5316455696203, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=29.0625, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.8675078864353, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=24.2214532871972, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=26.1980830670927, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=25.609756097561, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=26.25, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=27.5757575757576, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=21.5625, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36236; Mature: 36104

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHTPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRDEWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEECCCCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGRQSCELLFRLIAGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHTPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRDEWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEECCCCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGRQSCELLFRLIAGKPSPQNITVATHMTLK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]