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The map label for this gene is codB

Identifier: 209396382

GI number: 209396382

Start: 415500

End: 416759

Strand: Direct

Name: codB

Synonym: ECH74115_0409

Alternate gene names: 209396382

Gene position: 415500-416759 (Clockwise)

Preceding gene: 209400583

Following gene: 209399439

Centisome position: 7.46

GC content: 54.13

Gene sequence:

>1260_bases
GTGTCGCAAGATAACAACTTTAGCCAGGGGCCGGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCAT
GCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCC
TCGCAGTTCTCATCGGTAATCTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACC
ACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTGCTGGGCGGCACCCAGGTTGG
CTGGTTTGGCGTCGGTGTGGCGATGTTTGCTATTCCGGTGGGCAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCG
TTTCCGGTTTACTGATGACCGTCACCGTCTTCTTTGGCATTTCGGCGCTGACGGTTCTTTCGTTGATTGCGGTTCCGGCT
ATCGCCTGTCTTGGCGGTTATTCCGTGTGGCTGGCCGTTAACGGCATGGGCGGCCTGGACGCCTTAAAAGCGGTCGTTCC
CGCACAACCGTTAGATTTCAATGTCGCGCTGGCGCTGGTTGTGGGGTCATTTATCAGTGCGGGCACGCTCACCGCTGACT
TTGTCCGCTTTGGTCGCAATGCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTT
ATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGGATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGTTGCC
TGCGATTGTGGTGCTGGGGCTAAATATCTGGACCACCAACGATAACGCGCTCTATGCGTCGGGTTTAGGTTTCGCCAACA
TTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGTGCATTATGGCTGTATAACAAT
TTTGTCGGCTGGTTGACCTTCCTTTCGGCAGCTATTCCTCCAGTGGGTGGCGTGATCATTGCCGACTATCTGATGAACCG
TCGCCGCTATGAGCACTTTGCGACCACGCGTATGATGAGTGTCAATTGGGTGGCGATTCTGGCGGTCGCCCTGGGGATTG
CTGCAGGCCACTGGTTACCGGGAATTGTTCCGGTCAACGCGGTATTAGGTGGCGCGCTGAGCTATCTGATCCTTAACCCG
ATTTTGAATCGTAAAACGACAGCAGCAATGACGCATGTGGAGGCTAACAGTGTCGAATAA

Upstream 100 bases:

>100_bases
TAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAA
CAGCAAAAAGGGGGAATTTC

Downstream 100 bases:

>100_bases
CGCTTTACAAACAATTATTAACGCCCGGTTGCCAGGCAAAGAGGGGCTGTGGCAGATTCATCTGCAGGACGGAAAAATCA
GCGCCATTGATGCGCAATCC

Product: cytosine permease

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLGTGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLT
THLLARFSFGVKGSWLPSLLLGGTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFGRNAKLAVLVAMVAFFLGNSLMF
IFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNIWTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNN
FVGWLTFLSAAIPPVGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGALSYLILNP
ILNRKTTAAMTHVEANSVE

Sequences:

>Translated_419_residues
MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLGTGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLT
THLLARFSFGVKGSWLPSLLLGGTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFGRNAKLAVLVAMVAFFLGNSLMF
IFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNIWTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNN
FVGWLTFLSAAIPPVGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGALSYLILNP
ILNRKTTAAMTHVEANSVE
>Mature_418_residues
SQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLGTGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTT
HLLARFSFGVKGSWLPSLLLGGTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPAI
ACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFGRNAKLAVLVAMVAFFLGNSLMFI
FGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNIWTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNF
VGWLTFLSAAIPPVGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGALSYLILNPI
LNRKTTAAMTHVEANSVE

Specific function: Required for cytosine transport into the cell

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family

Homologues:

Organism=Escherichia coli, GI1786530, Length=419, Percent_Identity=99.7613365155131, Blast_Score=813, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CODB_ECO57 (Q8X691)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85528
- PIR:   E90677
- RefSeq:   NP_286077.1
- RefSeq:   NP_308416.1
- ProteinModelPortal:   Q8X691
- EnsemblBacteria:   EBESCT00000026409
- EnsemblBacteria:   EBESCT00000058211
- GeneID:   914491
- GeneID:   957261
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0432
- KEGG:   ecs:ECs0389
- GeneTree:   EBGT00050000010724
- HOGENOM:   HBG371811
- OMA:   MWTGGSL
- ProtClustDB:   PRK11017
- BioCyc:   ECOL83334:ECS0389-MONOMER
- InterPro:   IPR001248
- InterPro:   IPR012681
- TIGRFAMs:   TIGR00800

Pfam domain/function: PF02133 Transp_cyt_pur

EC number: NA

Molecular weight: Translated: 43664; Mature: 43533

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x253674d0)-; HASH(0x12a670a8)-; HASH(0xc352a38)-; HASH(0x252122e8)-; HASH(0x23d14834)-; HASH(0x24e3d900)-; HASH(0x24a0cd9c)-; HASH(0x24cbb960)-; HASH(0x252219b4)-; HASH(0x24f192cc)-; HASH(0x25036c48)-; HASH(0x25107574)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLGTGLSYHDFFLAVLIGN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLGGTQVGWFGVGVAMFAIPV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCC
GKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPAIACLGGYSVWLAVNGMGGLD
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
ALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFGRNAKLAVLVAMVAFFLGNSLMF
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEE
IFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNIWTTNDNALYASGLGFANITGMSSK
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCEEECCCCHH
TLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPPVGGVIIADYLMNRRRYEHFATTRMMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VNWVAILAVALGIAAGHWLPGIVPVNAVLGGALSYLILNPILNRKTTAAMTHVEANSVE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCH
>Mature Secondary Structure 
SQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLGTGLSYHDFFLAVLIGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLGGTQVGWFGVGVAMFAIPV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCC
GKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPAIACLGGYSVWLAVNGMGGLD
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
ALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFGRNAKLAVLVAMVAFFLGNSLMF
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEE
IFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNIWTTNDNALYASGLGFANITGMSSK
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCEEECCCCHH
TLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPPVGGVIIADYLMNRRRYEHFATTRMMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VNWVAILAVALGIAAGHWLPGIVPVNAVLGGALSYLILNPILNRKTTAAMTHVEANSVE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796