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The map label for this gene is zwf

Identifier: 209396330

GI number: 209396330

Start: 2442964

End: 2444439

Strand: Reverse

Name: zwf

Synonym: ECH74115_2587

Alternate gene names: 209396330

Gene position: 2444439-2442964 (Counterclockwise)

Preceding gene: 209399722

Following gene: 209399326

Centisome position: 43.87

GC content: 51.69

Gene sequence:

>1476_bases
ATGGCGGTAACGCAAACAGCCCAGGTCTGTGACTTGGTCATTTTCGGCGCGAAAGGCGACCTTGCGCGTCGTAAATTGCT
GCCTTCCCTGTATCAACTGGAAAAAGCCGGTCAGCTCAACCCGGACACCCGGATTATCGGCGTAGGGCGTGCTGACTGGG
ATAAAGCGGCATATACCAAAGTTGTCCGCGAGGCGCTCGAAACTTTCATGAAAGAAACCATTGATGAAGGTTTATGGGAC
ACCCTGAGCGCACGTCTGGATTTTTGTAATCTCGATGTCAATGACACTGCTGCATTCAGCCGTCTCGGCGCGATGCTGGA
TCAAAAAAATCGTATCACCATTAACTACTTTGCCATGCCGCCCAGCACTTTTGGCGCAATTTGCAAAGGGCTTGGCGAGG
CAAAACTGAATGCTAAACCGGCACGCGTAGTCATGGAGAAACCGCTGGGGACGTCGCTGGCGACCTCGCAGGAAATCAAT
GATCAGGTTGGCGAATACTTCGAGGAGTGCCAGGTTTACCGTATCGACCACTATCTTGGTAAAGAAACGGTGCTGAACCT
GTTGGCGCTGCGTTTTGCTAACTCCCTGTTTGTGAATAACTGGGACAATCGCACCATTGATCATGTTGAGATTACCGTGG
CAGAAGAAGTGGGGATCGAAGGGCGCTGGGGCTATTTTGATAAAGCCGGTCAGATGCGCGACATGATCCAGAACCACCTG
CTGCAAATTCTTTGCATGATTGCGATGTCTCCGCCGTCTGACCTGAGCGCAGACAGCATCCGCGATGAAAAAGTGAAAGT
ACTGAAGTCTCTGCGCCGCATCGACCGCTCCAACGTACGCGAAAAAACCGTACGCGGGCAATATACTGCGGGCTTCGCCC
AGGGCAAAAAAGTGCCGGGATATCTGGAAGAAGAGGGCGCGAACAAGAGCAGCAATACAGAAACCTTCGTGGCGATCCGC
GTCGACATTGATAACTGGCGCTGGGCCGGTGTGCCATTCTACCTGCGTACTGGTAAACGTCTGCCGACCAAATGTTCTGA
AGTCGTGGTCTATTTCAAAACACCTGAACTGAATCTGTTTAAAGAGTCGTGGCAGGATCTGCCGCAGAATAAACTGACTA
TCCGTCTGCAACCTGATGAAGGCGTGGATATCCAGGTACTGAATAAAGTTCCTGGCCTTGACCACAAACATAACCTGCAA
ATCACCAAGCTGGATCTGAGCTATTCAGAAACCTTTAATCAGACGCATCTGGCGGATGCCTATGAACGTTTGCTGCTGGA
AACCATGCGTGGTATTCAGGCACTGTTTGTACGTCGCGATGAAGTGGAAGAAGCCTGGAAATGGGTAGACTCCATTACTG
AGGCGTGGGCGATGGACAATGATGCGCCGAAACCGTATCAGGCCGGAACCTGGGGACCCGTTGCCTCGGTGGCGATGATT
ACCCGTGATGGTCGTTCCTGGAATGAGTTTGAGTAA

Upstream 100 bases:

>100_bases
ACAGTTTTCGCAAGCTCGTAAAAGCAGTACAGTGCACCGTAAGAAAATTACAAGTATACCCTGGCTTAAGTACCGGGTTA
GTTAACTTAAGGAGAATGAC

Downstream 100 bases:

>100_bases
TATCTGCGCTTATCCTTTATGGTTATTTTACCGGTAACATGATCTTGCACAGATTGTAGAACAATTTTTACACTTTCAGG
CCTCGTGCGGATTCACCCAC

Product: glucose-6-phosphate 1-dehydrogenase

Products: NA

Alternate protein names: G6PD

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWD
TLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHL
LQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIR
VDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQ
ITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMI
TRDGRSWNEFE

Sequences:

>Translated_491_residues
MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWD
TLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHL
LQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIR
VDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQ
ITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMI
TRDGRSWNEFE
>Mature_490_residues
AVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDT
LSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIND
QVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLL
QILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRV
DIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQI
TKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMIT
RDGRSWNEFE

Specific function: Pentose phosphate pathway; first step. [C]

COG id: COG0364

COG function: function code G; Glucose-6-phosphate 1-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-6-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI108773793, Length=485, Percent_Identity=37.1134020618557, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI109389365, Length=485, Percent_Identity=37.1134020618557, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI52145310, Length=496, Percent_Identity=27.0161290322581, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788158, Length=491, Percent_Identity=99.79633401222, Blast_Score=1019, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538218, Length=485, Percent_Identity=36.2886597938144, Blast_Score=302, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6324088, Length=477, Percent_Identity=34.5911949685535, Blast_Score=276, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24643352, Length=471, Percent_Identity=36.3057324840764, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24643350, Length=471, Percent_Identity=36.3057324840764, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI221513548, Length=486, Percent_Identity=27.9835390946502, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): G6PD_ECO57 (Q8XCJ6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90949
- PIR:   F85797
- RefSeq:   NP_288289.1
- RefSeq:   NP_310589.1
- ProteinModelPortal:   Q8XCJ6
- SMR:   Q8XCJ6
- EnsemblBacteria:   EBESCT00000029042
- EnsemblBacteria:   EBESCT00000056133
- GeneID:   912956
- GeneID:   961825
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2904
- KEGG:   ecs:ECs2562
- GeneTree:   EBGT00050000010896
- HOGENOM:   HBG322449
- OMA:   VVRGNAT
- ProtClustDB:   PRK05722
- BioCyc:   ECOL83334:ECS2562-MONOMER
- GO:   GO:0005488
- InterPro:   IPR001282
- InterPro:   IPR019796
- InterPro:   IPR022675
- InterPro:   IPR022674
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23429
- PIRSF:   PIRSF000110
- PRINTS:   PR00079
- TIGRFAMs:   TIGR00871

Pfam domain/function: PF02781 G6PD_C; PF00479 G6PD_N

EC number: =1.1.1.49

Molecular weight: Translated: 55733; Mature: 55602

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00069 G6P_DEHYDROGENASE

Important sites: ACT_SITE 239-239 BINDING 18-18 BINDING 50-50 BINDING 177-177 BINDING 181-181 BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTK
CCCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
VVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECC
PSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLG
CHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHC
KETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHL
HHHHHHHHHHHHHHHHEEECCCCCEECEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHH
LQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCC
YLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLF
CHHHCCCCCCCCCEEEEEEEEEECCCEECCCCEEEECCCCCCCCCCCEEEEEECCCCHHH
KESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADA
HHHHHHCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCHHHHCCHHHHHHH
YERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEE
TRDGRSWNEFE
EECCCCCCCCC
>Mature Secondary Structure 
AVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTK
CCCCHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHH
VVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECC
PSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLG
CHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEHHHHHC
KETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHL
HHHHHHHHHHHHHHHHEEECCCCCEECEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHH
LQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCC
YLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLF
CHHHCCCCCCCCCEEEEEEEEEECCCEECCCCEEEECCCCCCCCCCCEEEEEECCCCHHH
KESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADA
HHHHHHCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCHHHHCCHHHHHHH
YERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEE
TRDGRSWNEFE
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796