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The map label for this gene is rluE

Identifier: 209396285

GI number: 209396285

Start: 1552698

End: 1553321

Strand: Reverse

Name: rluE

Synonym: ECH74115_1596

Alternate gene names: 209396285

Gene position: 1553321-1552698 (Counterclockwise)

Preceding gene: 209397717

Following gene: 209399573

Centisome position: 27.88

GC content: 51.76

Gene sequence:

>624_bases
ATGCAAAAAACTTCTTTTAGAAATCACCAGGTTAAGCGATTCAGCTCACAACGTTCTACCAGGCGTAAACCTGAAAACCA
GCCCACGCGTGTGATCCTGTTCAATAAACCCTACGATGTTCTTCCGCAGTTCACCGATGAAGCCGGACGCAAAACATTAA
AAGAATTCATCCCGGTTCAGGGCGTTTATGCAGCAGGTCGTCTTGACCGCGATAGCGAAGGGTTACTGGTGCTGACCAAT
AACGGCGCGTTGCAGGCGCGTTTAACCCAGCCGGGCAAACGCACCGGAAAAATCTATTATGTGCAGGTGGAAGGTATTCC
CACACAAGACGCACTTGAAGCCTTGCGCAATGGCGTAACCTTAAATGATGGCCCTACCCTGCCCGCAGGCGCGGAGCTGG
TTGACGAACCCGCGTGGTTATGGCCGCGGAATCCACCAATTCGTGAACGCAAAAGTATTCCCACCAGCTGGCTGAAGATC
ACCTTATATGAAGGACGTAATCGCCAGGTGCGCCGCATGACCGCCCATGTTGGTTTCCCCACGCTGCGACTGATTCGCTA
TGCGATGGGTGATTACTCTTTGGATAACCTTGCCAATGGCGAATGGCGAGAAGCAACAGAATAA

Upstream 100 bases:

>100_bases
GATTTGTAATTGGCTTGTCAATACTACCCTATTGTTTGCCGTCATGAAAGATGCGCGTTATTAGGCTATAATGCGGCAAT
TCATAATCTCTGAAAATACC

Downstream 100 bases:

>100_bases
GGATGCACAATGTTTAAACCGCACGTTACCGTTGCTTGCGTAGTGCACGCAGAGGGGAAATTTTTAGTCGTTGAAGAGAC
GATTAATGGTAAAGCCTTAT

Product: 23S rRNA pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTN
NGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE

Sequences:

>Translated_207_residues
MQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTN
NGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE
>Mature_207_residues
MQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTN
NGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKI
TLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family

Homologues:

Organism=Escherichia coli, GI87081838, Length=207, Percent_Identity=99.0338164251208, Blast_Score=422, Evalue=1e-120,
Organism=Escherichia coli, GI1787524, Length=192, Percent_Identity=33.8541666666667, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788510, Length=182, Percent_Identity=33.5164835164835, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790453, Length=184, Percent_Identity=29.3478260869565, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUE_ECO57 (Q8X724)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85687
- PIR:   G90829
- RefSeq:   NP_287349.1
- RefSeq:   NP_309634.2
- ProteinModelPortal:   Q8X724
- SMR:   Q8X724
- EnsemblBacteria:   EBESCT00000025908
- EnsemblBacteria:   EBESCT00000055466
- GeneID:   913282
- GeneID:   959820
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1864
- KEGG:   ecs:ECs1607
- GeneTree:   EBGT00050000009689
- HOGENOM:   HBG754478
- OMA:   CQFSPHE
- ProtClustDB:   PRK11394
- BioCyc:   ECOL83334:ECS1607-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 23646; Mature: 23646

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01149 PSI_RSU

Important sites: ACT_SITE 79-79

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCC
GVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVT
CEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCEE
LNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCHHHHCCCCEECCCCCCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCH
TLRLIRYAMGDYSLDNLANGEWREATE
HHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIPVQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHCCCC
GVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVT
CEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHCCEE
LNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQVRRMTAHVGFP
ECCCCCCCCCHHHHCCCCEECCCCCCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCH
TLRLIRYAMGDYSLDNLANGEWREATE
HHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796