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The map label for this gene is rdoA

Identifier: 209396233

GI number: 209396233

Start: 4941482

End: 4942468

Strand: Direct

Name: rdoA

Synonym: ECH74115_5299

Alternate gene names: 209396233

Gene position: 4941482-4942468 (Clockwise)

Preceding gene: 209399915

Following gene: 209400816

Centisome position: 88.68

GC content: 48.23

Gene sequence:

>987_bases
ATGAACAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCAAGGGATCCGGGT
GGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCG
TCAAATTTTATCGTCCTGAACGTTGGACAGCCGATCAAATCCTCGAAGAACATCAATTTGCGTTGCAGCTGGTAAATGAT
GAAGTTCCGGTTGCAGCACCTGTGGCTTTTAACGGTCAGACTTTATTGAATCATCAGGGATTTTATTTCGCTGTTTTTCC
AAGCGTCGGTGGTCGCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACC
AGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTCTGAATGAATATCTCATTGAGCCACGCAAGCTGTTT
GAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATCGCCGCCGTTACAGC
ACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAA
TGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCTATTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAG
CAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGA
ACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCCGCGTTCCCGAAAAATT
TCCCGTGGTTAACCGGGGAAGATTACTGGCTACGACAGACGGCGACTTTTATAGAACAGGCAAAAGTTCTACAAGAACCC
CCTTTGCAATTAACACCTATGTATTAA

Upstream 100 bases:

>100_bases
GCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCATCATTCGT
AATGTTTTCCGGATGATGGG

Downstream 100 bases:

>100_bases
TCGGAGAGAGTAGATCATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGCATCGGCGGCGCAGT
ATGAAGATGGTAAACAGTAC

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVND
EVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDLDDARNGPAIQDLWMLLNGDKAE
QRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEP
PLQLTPMY

Sequences:

>Translated_328_residues
MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVND
EVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDLDDARNGPAIQDLWMLLNGDKAE
QRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEP
PLQLTPMY
>Mature_328_residues
MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVND
EVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDLDDARNGPAIQDLWMLLNGDKAE
QRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEP
PLQLTPMY

Specific function: Probably involved in the extracytoplasmic stress response

COG id: COG2334

COG function: function code R; Putative homoserine kinase type II (protein kinase fold)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rdoA family

Homologues:

Organism=Escherichia coli, GI1790290, Length=328, Percent_Identity=99.390243902439, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RDOA_ECO57 (Q8X8H9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D86073
- PIR:   F91226
- RefSeq:   NP_290484.1
- RefSeq:   NP_312809.1
- ProteinModelPortal:   Q8X8H9
- SMR:   Q8X8H9
- EnsemblBacteria:   EBESCT00000026867
- EnsemblBacteria:   EBESCT00000058594
- GeneID:   915109
- GeneID:   960301
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5391
- KEGG:   ecs:ECs4782
- GeneTree:   EBGT00050000009809
- HOGENOM:   HBG307327
- OMA:   VQDLWMM
- ProtClustDB:   PRK11768
- BioCyc:   ECOL83334:ECS4782-MONOMER
- GO:   GO:0005737
- InterPro:   IPR002575
- InterPro:   IPR011009

Pfam domain/function: PF01636 APH; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 38126; Mature: 38126

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPE
CCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHCCEEECCCCCCCEEEEEEECCC
RWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNID
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCEEEEEECCCCCCEECCCCHH
QMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDE
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHH
LIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDLDDARNGPAIQDLWMLLNGDKAE
HHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHH
QRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DYWLRQTATFIEQAKVLQEPPLQLTPMY
CHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPE
CCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHCCEEECCCCCCCEEEEEEECCC
RWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFYFAVFPSVGGRQFEADNID
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCEEEEEECCCCCCEECCCCHH
QMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDE
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHH
LIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDLDDARNGPAIQDLWMLLNGDKAE
HHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHCCCHHH
QRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVYYLAWLMRRWADPAFPKNFPWLTGE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DYWLRQTATFIEQAKVLQEPPLQLTPMY
CHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796