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The map label for this gene is dosC

Identifier: 209396130

GI number: 209396130

Start: 2001591

End: 2002973

Strand: Reverse

Name: dosC

Synonym: ECH74115_2103

Alternate gene names: 209396130

Gene position: 2002973-2001591 (Counterclockwise)

Preceding gene: 209396682

Following gene: 209400569

Centisome position: 35.95

GC content: 44.61

Gene sequence:

>1383_bases
ATGGAGATGTATTTTAAAAGAATGAAAGATGAGTGGACCGGACTTGTCGAGCAGGCAGATCCGCCCATTCGTGCTAAAGC
CGCGGAAATTGCCGTTGCGCATGCTCATTATCTGAGTATCGAGTTTTATCGAATTGTCCGCATCGACCCGCATGCCGAAG
AATTCTTGAGTAATGAACAAGTTGAGCGGCAGTTGAAAAGTGCGATGGAACGCTGGATTATTAACGTGCTTTCTGCCCAG
GTTGACGATGTCGAAAGGCTAATACAAATCCAGCATACCGTCGCGGAAGTGCATGCCCGCATAGGAATTCCGGTAGAAAT
TGTCGAGATGGGGTTTCGGGTGCTGAAAAAGATCCTCTATCCGGTAATCTTCTCTTCGGATTATTCTGCCGCAGAAAAAC
TTCAGGTCTACCATTTCTCGATTAACAGTATTGATATCGCTATGGAAGTGATGACCCGCGCGTTTACCTTTAGTGACAGT
AGTGCCTCAAAAGAAGATGAAAACTATCGTATCTTCTCGTTACTGGAAAACGCCGAAGAAGAAAAAGAACGGCAAATAGC
CTCAATACTTTCATGGGAAATAGATATTATCTATAAAATCCTGCTGGATTCTGATTTAGGCAGTAGTTTGCCTTTAAGCC
AGGCTGATTTTGGCCTGTGGTTTAACCATAAAGGTCGACATTATTTCAGCGGCATTGCTGAAGTGGGCCATATCTCTCGT
CTGATTCAGGATTTCGACGGTATTTTCAATCAAACCATGCGTAACACCAGGAATTTGAATAACAGAAGTCTGCGGGTGAA
ATTTTTATTACAGATAAGAAATACCGTATCGCAAATTATTACCTTGCTGCGTGAATTGTTTGAAGAAGTATCGCGCCACG
AAGTCGGTATGGATGTACTGACGAAATTACTTAACCGCCGTTTCCTGCCGACTATCTTCAAACGCGAAATTGCCCATGCC
AACCGGACCGGTACACCGCTGTCAGTGCTGATTATTGACGTTGATAAATTCAAAGAGATCAACGATACGTGGGGCCATAA
CACTGGCGATGAAATTCTGCGTAAAGTCTCTCAGGCCTTTTATGACAACGTCCGCAGTAGTGATTATGTTTTCCGCTACG
GGGGCGATGAATTTATCATTGTTTTGACTGAAGCTTCTGAAAACGAAACGTTACGTACCGCAGAACGTATTCGCAGTCGG
GTGGAGAAAACCAAACTGAAAGCCGCAAACGGCGAAGATATTGCCCTCTCACTTTCCATCGGTGCCGCCATGTTTAATGG
TCATCCTGACTATGAGCGCCTCATTCAAATAGCCGATGAAGCTCTGTATATCGCCAAAAGACGAGGTAGAAACCGTGTTG
AACTCTGGAAAGCCAGTCTTTAG

Upstream 100 bases:

>100_bases
ATCTTCATATTTCAGGAGAATATGGTGCAGTTAGTCGATCTCGCACGTCGCGTTTCATTTTGATTCCGTCTGATTTCCCA
GCCTTATAAGGGTGTGAATT

Downstream 100 bases:

>100_bases
ATGCGCCAGGATGCAGAGGTAATCATGAAGCTAACCGATGCGGATAATGCCGCCGATGGCATTTTTTTCCCCGCCCTTGA
GCAAAATATGATGGGTGCGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQ
VDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSR
VEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL

Sequences:

>Translated_460_residues
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQ
VDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSR
VEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
>Mature_460_residues
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQ
VDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSR
VEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI145693134, Length=460, Percent_Identity=100, Blast_Score=938, Evalue=0.0,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=34.5911949685535, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=36.5269461077844, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=35.2941176470588, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=31.5789473684211, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=33.1360946745562, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=35.8024691358025, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=33.1210191082803, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788956, Length=153, Percent_Identity=32.6797385620915, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=31.5217391304348, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=33.587786259542, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=29.2817679558011, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DOSC_ECOLI (P0AA89)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64902
- RefSeq:   AP_002113.1
- RefSeq:   NP_416007.3
- ProteinModelPortal:   P0AA89
- SMR:   P0AA89
- STRING:   P0AA89
- EnsemblBacteria:   EBESCT00000000518
- EnsemblBacteria:   EBESCT00000018191
- GeneID:   945835
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5241
- KEGG:   eco:b1490
- EchoBASE:   EB3554
- EcoGene:   EG13793
- eggNOG:   COG2199
- GeneTree:   EBGT00050000008776
- HOGENOM:   HBG493165
- OMA:   SEFGLWF
- ProtClustDB:   CLSK880042
- BioCyc:   EcoCyc:G6784-MONOMER
- Genevestigator:   P0AA89
- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050
- Gene3D:   G3DSA:1.10.490.10
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00990 GGDEF; SSF55073 A/G_cyclase; SSF46458 Globin_like

EC number: =2.7.7.65

Molecular weight: Translated: 53178; Mature: 53178

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50887 GGDEF

Important sites: ACT_SITE 376-376 BINDING 341-341 BINDING 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHCHHH
VERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
PVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEE
HHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
EKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAF
HHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
YDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSI
HHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRIVRIDPHAEEFLSNEQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHCHHH
VERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIGIPVEIVEMGFRVLKKILY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
PVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSSASKEDENYRIFSLLENAEE
HHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
EKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
LIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAF
HHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHH
YDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSI
HHCCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
CHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503