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The map label for this gene is gadC

Identifier: 209396109

GI number: 209396109

Start: 2004800

End: 2006335

Strand: Reverse

Name: gadC

Synonym: ECH74115_2105

Alternate gene names: 209396109

Gene position: 2006335-2004800 (Counterclockwise)

Preceding gene: 209399389

Following gene: 209396682

Centisome position: 36.01

GC content: 48.31

Gene sequence:

>1536_bases
ATGGCTACATTAGTACAGACAGGTAAAGCTAAGCAGCTCACATTACTCGGCTTTTTTGCCATAACGGCATCGATGGTAAT
GGCTGTTTATGAATACCCTACCTTCGCAACATCGGGCTTTTCATTAGTCTTCTTCCTGCTATTAGGCGGGATTTTATGGT
TTATTCCCGTGGGACTTTGTGCTGCGGAAATGGCCACCGTCGACGGCTGGGAAGAAGGTGGTGTCTTCGCCTGGGTATCA
AATACTCTGGGGCCGAGATGGGGATTTGCAGCGATCTCATTTGGCTATCTGCAAATCGCCATTGGTTTTATTCCGATGCT
CTATTTCGTGTTAGGGGCACTCTCCTACATCCTGAAATGGCCAGCGCTGAATGAAGACCCCATTACCAAAACTATTGCAG
CACTCATCATTCTTTGGGCGCTGGCATTAACGCAGTTTGGTGGCACGAAATACACGGCGCGAATTGCTAAAGTTGGCTTC
TTCGCCGGTATCCTGTTACCTGCATTTATTTTGATTGCATTAGCGGCTATTTATCTGCACTCCGGTGCCCCCGTTGCTAT
CGAAATGGATTCGAAGACCTTCTTCCCTGACTTCTCTAAAGTGGGCACCCTGGTTGTATTTGTTGCCTTCATTTTGAGTT
ATATGGGCGTAGAAGCTTCCGCAACCCACGTCAATGAAATGAGTAACCCAGGGCGCGACTATCCACTGGCTATGTTACTG
CTGATGGTGGCGGCAATCTGCTTAAGCTCTGTTGGCGGTTTGTCTATTGCGATGGTCATTCCGGGTAATGAAATCAACCT
CTCCGCAGGGGTAATGCAAACCTTTACCGTTCTGATGTCCCATGTGGCACCGGAAATTGAGTGGACGGTTCGCGTGATCT
CCGCACTGCTGTTGCTGGGTGTTCTGGCGGAAATCGCCTCCTGGATTGTTGGTCCTTCTCGCGGGATGTATGTCACAGCG
CAGAAAAACCTGCTGCCAGCGGCATTCGCTAAAATGAACAAAAATGGCGTACCGGTAACGCTGGTCATTTCGCAGCTGGT
GATTACTTCTATCGCGTTGATCATCCTCACCAATACCGGTGGCGGTAACAACATGTCCTTCCTGATCGCACTGGCGCTGA
CGGTGGTGATTTATCTGTGTGCTTATTTCATGCTGTTTATTGGCTACATTGTGTTGGTTCTTAAACATCCTGACTTAAAA
CGCACATTTAATATCCCTGGTGGTAAAGGGGTGAAACTGGTCGTGGCAATTGTCGGTCTGCTGACTTCAATTATGGCGTT
TATTGTTTCCTTCCTGCCGCCGGATAACATCCAGGGTGACTCTACCGATATGTATGTTGAATTACTGGTCGTTAGTTTCC
TGGTGGTACTTGCCCTGCCCTTTATTCTCTATGCTGTTCATGATCGTAAAGGCAAAGCGAATACCGGCGTCACTCTGGAG
CCAATCAACAGTCAGAACGCACCAAAAGGTCACTTCTTCCTGCACCCGCGTGCACGTTCACCACACTATATTGTGATGAA
TGACAAGAAACACTAA

Upstream 100 bases:

>100_bases
AGTCTGTTCACTGGCATTAGCAACGGAAAATATTGTTCTGAATACGCTTCAGAACAAAACAGGTGCGGTTCCGACAGGAA
TACCGTTTTAGGGGGATAAT

Downstream 100 bases:

>100_bases
CTATGTCTGAATGGTTATAAGATAAGGGAGCGATAATTCATCGCTCCCTTTTTCGTGCTTGCTGTCGTATTGACTTAACC
GGGATAAGTACGAGAATGAG

Product: glutamate:gamma aminobutyrate antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: Extreme acid sensitivity protein

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVS
NTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKANTGVTLE
PINSQNAPKGHFFLHPRARSPHYIVMNDKKH

Sequences:

>Translated_511_residues
MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVS
NTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKANTGVTLE
PINSQNAPKGHFFLHPRARSPHYIVMNDKKH
>Mature_510_residues
ATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSN
TLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFF
AGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLLL
MVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQ
KNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKR
TFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKANTGVTLEP
INSQNAPKGHFFLHPRARSPHYIVMNDKKH

Specific function: Involved in glutamate-dependent acid resistance. Imports glutamate inside the cell while simultaneously exporting to the periplasm the GABA produced by gadA and gadB. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family

Homologues:

Organism=Escherichia coli, GI1787768, Length=511, Percent_Identity=99.8043052837573, Blast_Score=1019, Evalue=0.0,
Organism=Escherichia coli, GI87082380, Length=436, Percent_Identity=24.3119266055046, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081798, Length=402, Percent_Identity=24.1293532338308, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GADC_ECO57 (P58229)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90891
- PIR:   H85726
- RefSeq:   NP_287663.1
- RefSeq:   NP_310124.1
- ProteinModelPortal:   P58229
- EnsemblBacteria:   EBESCT00000023967
- EnsemblBacteria:   EBESCT00000058978
- GeneID:   917297
- GeneID:   960676
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2216
- KEGG:   ecs:ECs2097
- GeneTree:   EBGT00050000009007
- HOGENOM:   HBG514881
- OMA:   TSSMVIS
- ProtClustDB:   CLSK876865
- BioCyc:   ECOL83334:ECS2097-MONOMER
- GO:   GO:0009405
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060
- TIGRFAMs:   TIGR00910

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 55104; Mature: 54972

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1fb0631c)-; HASH(0x2041cfdc)-; HASH(0x20c1d8b4)-; HASH(0x207a7f68)-; HASH(0x20c4fda0)-; HASH(0x20bd8cc0)-; HASH(0x20c88d10)-; HASH(0x20bf3c04)-; HASH(0x20be2d60)-; HASH(0x20a14340)-; HASH(0x20bd3a20)-; HASH(0x20ca3c2c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLC
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKW
HHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHH
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTG
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCC
GGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
LTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKANTGVTLE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
PINSQNAPKGHFFLHPRARSPHYIVMNDKKH
ECCCCCCCCCEEEECCCCCCCCEEEECCCCH
>Mature Secondary Structure 
ATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLC
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKW
HHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHH
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTG
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCC
GGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
LTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKANTGVTLE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
PINSQNAPKGHFFLHPRARSPHYIVMNDKKH
ECCCCCCCCCEEEECCCCCCCCEEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796