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The map label for this gene is puuB [H]

Identifier: 209396047

GI number: 209396047

Start: 1839055

End: 1840335

Strand: Direct

Name: puuB [H]

Synonym: ECH74115_1943

Alternate gene names: 209396047

Gene position: 1839055-1840335 (Clockwise)

Preceding gene: 209396804

Following gene: 209400873

Centisome position: 33.0

GC content: 58.94

Gene sequence:

>1281_bases
ATGACAGAACATACCAGCAGTTACTACGCCGCCAGTGCGAATAAATACGCGCCATTCGACACGCTGAATGAGTCAATTTC
CTGCGACGTTTGCGTGGTTGGCGGCGGCTATACCGGGCTGTCTTCCGCACTGCATCTGGCGGAAGCGGGCTTTGACGTGG
TGGTTCTGGAAGCCTCACGCATCGGCTTTGGCGCCAGCGGGCGCAACGGCGGGCAGCTTGTGAACTCCTACAGCCGCGAC
ATCGACGTTATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCAT
CCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGTCTGTTTGTGGCGATGAATGATAAACAGC
TCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAGATGCTGGACGCCAACGCCATT
CGCCGCGAGGTCGCCAGCGATCGCTACACTGGTGCGCTGCTGGATCACAGCGGCGGGCATATTCACCCGCTGAACCTCGC
CATTGGCGAAGCGGACGCTATCCGCCTGAACGGCGGGCGCGTGTATGAGCTTTCCGCAGTGACGCAGATCCAGCACACCA
CGCCCGCCGTAGTGCGTACAGCCACAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTACCTGGGCGAT
AAAGTGGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGTACGCAGGTGATCACCACCGAGCGGCTTTCCGATGATTT
AGCCCGTTCGCTGATCCCGAAAAACTACTGCGTGGAAGACTGTAATTATCTGCTTGATTACTACCGTCTTACCGCCGACA
ACCGTTTGCTGTACGGCGGCGGCGTGGTGTACGGCGCGCGCGACCCGGATGACGTTGAGCGTCTTGTGGTGCCGAAACTG
CTGAAAACCTTCCCGCAGTTGAAGGGCGTGAAAATCGACTATCGCTGGACGGGCAACTTCCTGCTGACCCTGTCGCGTAT
GCCGCAGTTCGGTCGCCTCGATACCAACATCTATTACATGCAGGGTTACAGCGGCCACGGCGTCACCTGTACTCATCTCG
CCGGACGTTTGATTGCCGAACTGCTGCGCGGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTC
CCCGGCGGGCGCACGCTGCGCGTACCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTA
A

Upstream 100 bases:

>100_bases
GGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCCTTGAAAAATTCACCGAACTGAAAACCATCTG
GATAAGCCTGGAGGCCTGAA

Downstream 100 bases:

>100_bases
TTTCCGATTAACAGTGAAGAGTCAAAAGGTGTGAAACATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCCACCC
CGCGCGGGGTTGGCGTGATG

Product: gamma-glutamylputrescine oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTATGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV

Sequences:

>Translated_426_residues
MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRD
IDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTATGQVTAKYVIVAGNAYLGD
KVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKL
LKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPF
PGGRTLRVPFTAMGAAYYSLRDRLGV
>Mature_425_residues
TEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDI
DVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAIR
REVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTATGQVTAKYVIVAGNAYLGDK
VEPELAKRSMPCGTQVITTERLSDDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLL
KTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFP
GGRTLRVPFTAMGAAYYSLRDRLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=99.0610328638498, Blast_Score=872, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47133; Mature: 47002

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCCEEECCCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTATGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCCC
KVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHH
RDRLGV
HHHHCC
>Mature Secondary Structure 
TEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
CCCCCCEEECCCCCCCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECC
IGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFEGGEIIRERIKRYQIDCDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHEEECCCC
RPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAIRREVASDRYTGALLDHSGGH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTATGQVTAKYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEEEEEHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCCC
KVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNYCVEDCNYLLDYYRLTADNRLLYGG
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
GVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQFGRLDTNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEE
QGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRVPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHH
RDRLGV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]