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The map label for this gene is yafL [H]

Identifier: 209395992

GI number: 209395992

Start: 287214

End: 287987

Strand: Direct

Name: yafL [H]

Synonym: ECH74115_0271

Alternate gene names: 209395992

Gene position: 287214-287987 (Clockwise)

Preceding gene: 209399792

Following gene: 209398973

Centisome position: 5.15

GC content: 52.2

Gene sequence:

>774_bases
ATGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTT
GCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTG
CGCGTTTATTAAAACAGTACCAAACCCATCTGAAAAAGCAGGTCAGCTATATTGTGGAAGGCAATGCCGAAAGCAGAAGG
GCGCTACGCCAGCACAACCGCGAACAGATAAAACAGCATCCAGAATGGTTTCCTGCACCGCTCAAGGCGAGTGACAGACG
CTGGCAGGCGCTGGCGGAAAACAACCACTTTTTAAGCAGCGACCATCTGCATAACATTACCGAAGTGGCGATTCACCGCC
TGGAGCAGCAGCTTGGCAAGCCTTACGTCTGGGGCGGCACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTT
TATGCCTACAACAAGATCCTCGAGGCGAAGCTCCCGCGTACGGCTAATGAGATGTATCACTACCATCGGGCAACGATTGT
GGCGAATAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATCCACAGCCGCGAGATTGCCGACCATATGGGGGTGT
ATCTGGGCGACGGTCAGTTTATTGAGTCACCGCGCACCGGGGAAACCATTCGGGTAAGTCGGTTAGCTGAACCTTTCTGG
CAGGATCACTTTTTAGGCGCGCGCCGGATTTTGACGGAAGAGACGATTTTGTAG

Upstream 100 bases:

>100_bases
ACGGATGTTTTTACATAATTAACTGAAATATATTTATTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGGTACGGGC
CGGGATACACTGTAACATCA

Downstream 100 bases:

>100_bases
GACGGATAAGGCATTCACGCCGCATCCGGCAGTTGTGCGCAGATGCCTGATACGACGCTGACGCGTCTTATCATGCCTAC
AAATCTGTGCGTTTTTGCGA

Product: NlpC/P60 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MHDQPLLSMSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQVSYIVEGNAESRR
ALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVF
YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFW
QDHFLGARRILTEETIL

Sequences:

>Translated_257_residues
MHDQPLLSMSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQVSYIVEGNAESRR
ALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVF
YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFW
QDHFLGARRILTEETIL
>Mature_257_residues
MHDQPLLSMSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQVSYIVEGNAESRR
ALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVF
YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFW
QDHFLGARRILTEETIL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=249, Percent_Identity=98.3935742971888, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=51.4925373134328, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 29730; Mature: 29730

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDQPLLSMSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQY
CCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCC
DHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEMYH
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
YHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFW
HHHEEEEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHH
QDHFLGARRILTEETIL
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHDQPLLSMSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQY
CCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCC
DHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEMYH
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
YHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFW
HHHEEEEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHH
QDHFLGARRILTEETIL
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]