BacMap

Definition	Escherichia coli O157:H7 str. EC4115, complete genome.
Accession	NC_011353
Length	5,572,075

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The map label for this gene is ytbE [H]

Identifier: 209395979

GI number: 209395979

Start: 363291

End: 364160

Strand: Direct

Name: ytbE [H]

Synonym: ECH74115_0353

Alternate gene names: 209395979

Gene position: 363291-364160 (Clockwise)

Preceding gene: 209400580

Following gene: 209398835

Centisome position: 6.52

GC content: 42.99

Gene sequence:

>870_bases
GTGGAATTTTCCGTATTAAGTAACAATCTGAAAATGCCGATGATGGGATTTGGTGTTTTTCAGGTTACCGATAAAAACGT
GTGCAAACAGTCAGTGTTGAACGCTATCCGCACGGGGTATCGACTCATCGACACTGCAGCGGTATACGGTAATGAAGATG
CCGTCGGTGAAGCCGTTCGTGAAGCTATATCCGAAGGTTTATGTACCCGAGAAGAGTTATTTATAACGTCTAAATTGTGG
GTGCAGGATATGTTGAATCAGGATACGGCAGCAGCAGGTATTGAAGCATCATTAAAAAAATCGGGACTAGAATACTTCGA
CCTCTATTTATTGCATCAGGCTATGCGCGATTATTTCAGTGCGTGGCGTGCACTCGAAGATGCCTATGAAGAGGGCAAAT
TAAAAGCGATTGGGGTTTCCAATTTCTATCCTCATGTTCTGGCGAACTTTTGTGAAACGGTAAGAGTTAAACCGATGGTC
AACCAGGTCGAGTTGCATCCCTATTTTGCCCAACCAGAGGCGCTGGCAACCATGAAGTATTATAACGTGCAGCCTGAAGC
ATGGGCTCCGTTAGGTGGTGGACGACATAAACCCTTTGAAAATAATCTGCTTCAGAGTATTGCAGATGCCCATCAAAAAT
CGATTTCTCAAGTCATTCTGCGTTGGAATATTCAACGGGGAGTGGTCGTTATTCCGAAATCGACACATCAACAGCGTATC
GAAGAAAATTTTGCTATCTGGGATTTCTCACTGACAGAGAAAGAAATGGCACAAATTAGTTCGCTTGATTTGGGTTATGT
TGGGGAGTCGGTAAAACATTTTAATCCTGAATTTGTTCGTGGTTGTCTTGCTGTAAAAATACATGATTGA

Upstream 100 bases:

>100_bases
TTAATGAATTTTTTGCATAAGTGATATCAAAATCCACATACTAATTTGAGGTTACGTTTTAACGTAGACTCATTGTTCAT
GCCTAATGGAGGGACTGACA

Downstream 100 bases:

>100_bases
TATTAATCATGTATTTTACCTGAGACGACAAGAATCTTTTAACAGGGGAGTGATATTGATCTTCACTCTGTCATATCTCC
GGTAATATGGCGTCAGGCTT

Product: oxidoreductase, aldo/keto reductase family

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLW
VQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRI
EENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD

Sequences:

>Translated_289_residues
MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLW
VQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRI
EENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD
>Mature_289_residues
MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDAVGEAVREAISEGLCTREELFITSKLW
VQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRI
EENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=38.2252559726962, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI223468663, Length=295, Percent_Identity=36.6101694915254, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=34.6020761245675, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=34.6020761245675, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI300116271, Length=273, Percent_Identity=37.7289377289377, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=36.5942028985507, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI310109922, Length=265, Percent_Identity=35.8490566037736, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI45446745, Length=286, Percent_Identity=34.2657342657343, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI4503285, Length=286, Percent_Identity=34.2657342657343, Blast_Score=161, Evalue=6e-40,
Organism=Homo sapiens, GI24497583, Length=287, Percent_Identity=34.4947735191638, Blast_Score=160, Evalue=8e-40,
Organism=Homo sapiens, GI310109920, Length=286, Percent_Identity=33.9160839160839, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI24497585, Length=289, Percent_Identity=34.6020761245675, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI5453543, Length=286, Percent_Identity=33.9160839160839, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI291291012, Length=279, Percent_Identity=34.7670250896057, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=32.4324324324324, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4502049, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=35.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI207028673, Length=103, Percent_Identity=40.7766990291262, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI310109926, Length=103, Percent_Identity=39.8058252427184, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI87082198, Length=257, Percent_Identity=40.4669260700389, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786400, Length=274, Percent_Identity=32.1167883211679, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788081, Length=274, Percent_Identity=29.9270072992701, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994888, Length=282, Percent_Identity=25.177304964539, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17550248, Length=284, Percent_Identity=37.3239436619718, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=34.2465753424658, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.4166666666667, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=34.5724907063197, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17537075, Length=291, Percent_Identity=34.7079037800687, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17566692, Length=282, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=31.3868613138686, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=29.2929292929293, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17552492, Length=268, Percent_Identity=29.1044776119403, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=27.9411764705882, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17562292, Length=279, Percent_Identity=26.8817204301075, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=28.2051282051282, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17550246, Length=75, Percent_Identity=45.3333333333333, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321896, Length=304, Percent_Identity=35.1973684210526, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=32.9861111111111, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324694, Length=301, Percent_Identity=30.8970099667774, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6322556, Length=271, Percent_Identity=33.9483394833948, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319625, Length=312, Percent_Identity=30.7692307692308, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320079, Length=290, Percent_Identity=31.0344827586207, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=37.8839590443686, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24657054, Length=289, Percent_Identity=37.0242214532872, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=34.2560553633218, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24663317, Length=299, Percent_Identity=34.7826086956522, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129731, Length=287, Percent_Identity=33.7979094076655, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=31.25, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45553081, Length=288, Percent_Identity=29.8611111111111, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24662785, Length=272, Percent_Identity=34.9264705882353, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24662781, Length=277, Percent_Identity=34.2960288808664, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI281366140, Length=255, Percent_Identity=29.8039215686275, Blast_Score=128, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32704; Mature: 32704

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDAVGEAVR
CCCCEECCCCCCCEECCCEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHH
EAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMRDYFS
HHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEALATMKY
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHEEECCCCCCCHHHHHHHH
YNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCHHHHHH
EENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD
HCCCEEEEECCCHHHHHHHHCCCHHHHCCHHHHCCHHHHHCEEEEEECC
>Mature Secondary Structure
MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLIDTAAVYGNEDAVGEAVR
CCCCEECCCCCCCEECCCEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHH
EAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMRDYFS
HHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEALATMKY
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHEEECCCCCCCHHHHHHHH
YNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIPKSTHQQRI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCHHHHHH
EENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD
HCCCEEEEECCCHHHHHHHHCCCHHHHCCHHHHCCHHHHHCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]