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The map label for this gene is hemF [H]

Identifier: 209395878

GI number: 209395878

Start: 3380445

End: 3381344

Strand: Direct

Name: hemF [H]

Synonym: ECH74115_3666

Alternate gene names: 209395878

Gene position: 3380445-3381344 (Clockwise)

Preceding gene: 209397820

Following gene: 209399196

Centisome position: 60.67

GC content: 54.56

Gene sequence:

>900_bases
ATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGA
TGGCGCAGAATTTGTCGAAGATAGCTGGCAGCGCGAAGGTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTG
TTTTCGAACAGGCAGGCGTCAACTTTTCTCACGTCCACGGCGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAA
CTTGCCGGGCGCAGTTTCGAGGCGATGGGCGTTTCTCTGGTAGTGCATCCGCATAATCCGTATGTTCCCACCAGCCACGC
GAATGTGCGCTTTTTTATTGCCGAAAAACCGGGTGCAGAGCCAGTCTGGTGGTTTGGCGGCGGCTTCGATTTAACCCCTT
TCTATGGTTTTGAAGAAGACGCCATTCACTGGCACCGCACCGCCCGTGACCTGTGCCTGCCATTTGGTAAAGACGTTTAT
CCCCGTTACAAAAAGTGGTGCGACGATTACTTCTACCTCAAACATCGCAACGAACAGCGCGGTATTGGCGGGCTGTTCTT
TGATGATCTAAACACGCCAGATTTCGACCACTGTTTTGCCTTTATGCAGGCGGTAGGCAAAGGCTACACCGACGCTTATT
TACCAATTGTAGAGCGACGTAAAGCGATGGCCTACGGCGAGCGCGAGCGCAATTTTCAGCTCTACCGTCGCGGTCGTTAT
GTCGAGTTCAATCTGGTCTGGGATCGCGGCACTCTGTTTGGCCTGCAAACTGGCGGGCGCACCGAGTCGATCCTGATGTC
AATGCCGCCACTGGTACGCTGGGAGTATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTA
AGGTCAGGGATTGGGTGTAA

Upstream 100 bases:

>100_bases
GGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCAC
ATTCGAAAAAGCGATAAGTT

Downstream 100 bases:

>100_bases
CTCCCTCACCCCCATTCCCGCATCCGCTGATGCAACGTCAACGACGGCTTCTCGGCAAACAGCTGCTGGTAATCCGTGGC
AAATTGCCCCAGATGCCAGA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV

Sequences:

>Translated_299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
>Mature_299_residues
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGGRSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPE
LAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=303, Percent_Identity=47.5247524752475, Blast_Score=285, Evalue=4e-77,
Organism=Escherichia coli, GI1788777, Length=299, Percent_Identity=98.3277591973244, Blast_Score=615, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6320249, Length=317, Percent_Identity=43.5331230283912, Blast_Score=257, Evalue=1e-69,
Organism=Drosophila melanogaster, GI17864388, Length=304, Percent_Identity=48.3552631578947, Blast_Score=288, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 34289; Mature: 34289

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGGRSRVLRNGGVFEQAGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHCCC
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAE
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVYPRYKKWCDDYFYLKHRNEQR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEECCCCCC
GIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGGRSRVLRNGGVFEQAGV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHCCC
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAE
CEEEECCCCCCCCCCCCCCHHCCCCEEECCEEEEEECCCCCCCCCCCCEEEEEECCCCCC
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVYPRYKKWCDDYFYLKHRNEQR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEECCCCCC
GIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
CCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCE
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
EEEEEEECCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA