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The map label for this gene is ecpD [H]

Identifier: 209395800

GI number: 209395800

Start: 1284197

End: 1284886

Strand: Reverse

Name: ecpD [H]

Synonym: ECH74115_1272

Alternate gene names: 209395800

Gene position: 1284886-1284197 (Counterclockwise)

Preceding gene: 209397811

Following gene: 209399424

Centisome position: 23.06

GC content: 46.23

Gene sequence:

>690_bases
ATGAAATCCGTTTTGCTGCTCTTAACCATCTTGTTCGTACAGTCAGTGGAAGCCGGCATCATGCCGGCTTCTTCACGGAT
TATTTATCGCGATGGCGATCGGGAAAAGACACTGATGCTGGTCAATACCAATGACTATCCGGTACTGGTGCAAAGTTGGG
TAGATAATGGTGAAGGTTCCCCAGAAGCTGCTTCTCCCCCCTTTGTGGTGCTGCCACCGGTTTTCCGCCTGGCTGCGAGA
CGAACCCAGGGGTTGCGGATTATCTATAATCATGACCCACTTCCCCAGGATCGCGAATCTGCATTCTGGTTAAATCTTTA
TGAAATGCCGCCAGAAACTCCCCATCAAGATCCCACACATTTAACGCTGGCAATGAATACGCAACTTAAAATCTTTTACA
GGCCAAAATCTGTAACTATGACGCCCCAAGAAGCTATGCGTAAAATAAGTTGGCATCTGGGTAACGACGGCAGCAGTAGC
TATATTGAGCTTAATAACCCAACACCACTGAATATCTCTTTCACCTCCATCAGCATTGGGGCTACAAAGGTGCAGCAAGA
AAGCGATATGATGATAAAACCCTTCAGTCAACGACGGTACTGGTTAGCAAGTAATAACGTTAGTGGTGTGGTTGCGGCGA
CATATCTCGATGATAATGGTGCACAGATAAAACAACAATTAGCGCAATAA

Upstream 100 bases:

>100_bases
GCTATAGTTACTCTTTTATCGCCCGGTTGAAGAAACTGCCAAATCAGACAGTCAGTGCGGGAAAAGTGCGGGCAACGGCT
TATATATTGGTGAAAATGCA

Downstream 100 bases:

>100_bases
TGCTCTGAGTGGTATGTAAACTCATCGCGATGGTTCAACGCTAACGAATAACCACTATACCCACCTGAAGTACCGGAAGT
GGAGAAATGACAAAATCTCT

Product: gram-negative pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTNDYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAAR
RTQGLRIIYNHDPLPQDRESAFWLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDDNGAQIKQQLAQ

Sequences:

>Translated_229_residues
MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTNDYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAAR
RTQGLRIIYNHDPLPQDRESAFWLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDDNGAQIKQQLAQ
>Mature_229_residues
MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTNDYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAAR
RTQGLRIIYNHDPLPQDRESAFWLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDDNGAQIKQQLAQ

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=202, Percent_Identity=33.6633663366337, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788428, Length=230, Percent_Identity=29.5652173913043, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790771, Length=228, Percent_Identity=28.9473684210526, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787171, Length=213, Percent_Identity=31.4553990610329, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789532, Length=185, Percent_Identity=31.3513513513514, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082203, Length=211, Percent_Identity=30.8056872037915, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786743, Length=193, Percent_Identity=29.5336787564767, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786936, Length=223, Percent_Identity=29.1479820627803, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788677, Length=212, Percent_Identity=28.7735849056604, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081806, Length=204, Percent_Identity=25.4901960784314, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25778; Mature: 25778

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTNDYPVLVQSWVDNGEGS
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCC
PEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAFWLNLYEMPPETPHQDPTH
CCCCCCCEEEECHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCE
LTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSSYIELNNPTPLNISFTSISIG
EEEEECCEEEEEECCCCEEECHHHHHHHHHEECCCCCCCCEEECCCCCCEEEEEEEEECC
ATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDDNGAQIKQQLAQ
CEEECCCCCCEECCCCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTNDYPVLVQSWVDNGEGS
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCC
PEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAFWLNLYEMPPETPHQDPTH
CCCCCCCEEEECHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCE
LTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSSYIELNNPTPLNISFTSISIG
EEEEECCEEEEEECCCCEEECHHHHHHHHHEECCCCCCCCEEECCCCCCEEEEEEEEECC
ATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDDNGAQIKQQLAQ
CEEECCCCCCEECCCCCCEEEEECCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]