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The map label for this gene is yfbQ [H]

Identifier: 209395758

GI number: 209395758

Start: 3175093

End: 3176310

Strand: Direct

Name: yfbQ [H]

Synonym: ECH74115_3429

Alternate gene names: 209395758

Gene position: 3175093-3176310 (Clockwise)

Preceding gene: 209399997

Following gene: 209397835

Centisome position: 56.98

GC content: 50.41

Gene sequence:

>1218_bases
ATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACG
CCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAA
TCCTCGTTGACGTGATACGCAACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCC
ATCATGCAGCACTATCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCT
TATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTATGGACCG
CGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGAT
ATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAA
AGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTC
TCTACGACGACGCTGAGCATCACTCAATTGCGCCGCTGGCACCTGACCTGCTGACCATTACCTTTAATGGACTGTCGAAA
ACGTACCGCGTTGCAGGCTTCCGTCAGGGGTGGATGGTGTTGAACGGGCCGAAAAAACACGCCAAAGGCTACATCGAAGG
TCTGGAAATGCTGGCTTCAATGCGCCTGTGTGCTAACGTTCCTGCGCAACACGCCATTCAGACCGCGCTGGGTGGTTATC
AGAGCATCAGTGAATTTATTACCCCTGGCGGTCGTCTTTATGAGCAGCGTAACCGCGCGTGGGAATTGATCAACGATATT
CCGGGCGTTTCCTGCGTGAAACCTTGTGGTGCGCTGTACATGTTCCCGAAAATCGACGCCAAACGCTTTAACATTCACGA
CGATCAGAAAATGGTGCTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGC
CGGATCACTTCCGCATTGTCACGCTACCGCGTGTCGATGATATCGAGCTGTCGTTGAGCAAGTTCGCGCGTTTCCTTTCT
GGTTATCATCAGCTGTAA

Upstream 100 bases:

>100_bases
TATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAAC
AGCAAAAAAGGTCAAGATTC

Downstream 100 bases:

>100_bases
TCTTAATTTCACTGCCGGAGATTGCATCCGGCAGTGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTC
ACTATGAAACAGAGCCATTT

Product: aminotransferase AlaT

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKA
IMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDI
PGVSCVKPCGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLS
GYHQL

Sequences:

>Translated_405_residues
MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKA
IMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDI
PGVSCVKPCGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLS
GYHQL
>Mature_404_residues
SPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAI
MQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDI
RAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSKT
YRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIP
GVSCVKPCGALYMFPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSG
YHQL

Specific function: Unknown

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4507369, Length=382, Percent_Identity=26.4397905759162, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI19263340, Length=462, Percent_Identity=25.3246753246753, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI215599424, Length=368, Percent_Identity=26.6304347826087, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4885351, Length=447, Percent_Identity=23.7136465324385, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI95147551, Length=171, Percent_Identity=28.6549707602339, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI169881279, Length=171, Percent_Identity=28.6549707602339, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI56713254, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI56713256, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI169881281, Length=161, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI149944424, Length=343, Percent_Identity=22.4489795918367, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788627, Length=405, Percent_Identity=99.7530864197531, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1788722, Length=377, Percent_Identity=26.2599469496021, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786816, Length=263, Percent_Identity=25.0950570342205, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17567663, Length=369, Percent_Identity=29.2682926829268, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71981209, Length=458, Percent_Identity=25.764192139738, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994472, Length=205, Percent_Identity=29.7560975609756, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994476, Length=205, Percent_Identity=29.7560975609756, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71981215, Length=293, Percent_Identity=26.6211604095563, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17567369, Length=178, Percent_Identity=29.2134831460674, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320317, Length=360, Percent_Identity=27.5, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323118, Length=357, Percent_Identity=27.7310924369748, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322401, Length=303, Percent_Identity=24.0924092409241, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18859735, Length=375, Percent_Identity=26.6666666666667, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45551451, Length=470, Percent_Identity=26.8085106382979, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641770, Length=470, Percent_Identity=26.8085106382979, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641760, Length=470, Percent_Identity=26.8085106382979, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641768, Length=470, Percent_Identity=26.8085106382979, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641766, Length=470, Percent_Identity=26.8085106382979, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641764, Length=470, Percent_Identity=26.8085106382979, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28573069, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646114, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573067, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573065, Length=167, Percent_Identity=27.5449101796407, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.1

Molecular weight: Translated: 45465; Mature: 45334

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIR
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
EMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNP
CEEEECCCCCHHHHEEECCCCCEEEEEECCCCCCCCCHHHHHEEECCCCCEEEEECCCCC
TGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
CCHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCH
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFI
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
TPGGRLYEQRNRAWELINDIPGVSCVKPCGALYMFPKIDAKRFNIHDDQKMVLDFLLQEK
CCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCC
VLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSGYHQL
EEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPAPFGFDAPDEILVDVIR
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGNGVSELIVQAMQALLNSGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
EMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDDIRAKITPRTRGIVIINPNNP
CEEEECCCCCHHHHEEECCCCCEEEEEECCCCCCCCCHHHHHEEECCCCCEEEEECCCCC
TGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDAEHHSIAPLAPDLLTITFNGLSK
CCHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCH
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPAQHAIQTALGGYQSISEFI
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
TPGGRLYEQRNRAWELINDIPGVSCVKPCGALYMFPKIDAKRFNIHDDQKMVLDFLLQEK
CCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCC
VLLVQGTAFNWPWPDHFRIVTLPRVDDIELSLSKFARFLSGYHQL
EEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]