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The map label for this gene is NemA4 [C]

Identifier: 20808970

GI number: 20808970

Start: 2517575

End: 2519497

Strand: Reverse

Name: NemA4 [C]

Synonym: TTE2625

Alternate gene names: 20808970

Gene position: 2519497-2517575 (Counterclockwise)

Preceding gene: 20808971

Following gene: 20808969

Centisome position: 93.68

GC content: 42.64

Gene sequence:

>1923_bases
ATGGAGCTTACAAAACCCATAAAACTCGGGGGGATAGAGCTTAAAAACCGCCTTGTAATGGCTCCTGTTAAAACAGGGTA
TGCTACAGTAGAGGGGCAGGTTACGGAAGAATTGATAGGATATTATCTTAATAGAGCAAAGGGAGGGGTGGGTTTAATTG
TTTTAGAATCCGCATACGTGGATCCATCCGGCAAAGAAATTCCGCGCCAGTTAGGGATTTACGATGATTCGCTGGTAGAT
GGATTAAAACGCCTTGTTACCCCCCTACAGGAGGCTGGTAGCAAAGTCGCAGTATATATCAACCATGCTGGAAGAGTTGC
AAATCCCATGGCGACGAAAATGCCTCTTATTGCGCCTTCTGCTGTAATGTGCCCGAGCATTGGCGTAGAGCCTAAAGCAA
TAGAAAAAGAAGAAATAAAAAAATGGCAGAAGGCCTATGTGGATGCTGCGATAAGGGCTTATAAAGCAGGGGCAGACTTT
TTGGAGATACAGTTTGGACATGGTTATCTTATTCACCAATTTTTATCTCCTCATGCCAACAAAAGGCAGGACGAATACGG
TGGGAGTTTTGAGAATAGAATAAGATTTGGGATAGAAATTTTAGAAGAAATTAGAAGTGAATTACCCGGTTTTCCTGTAA
TTGTTAGAATAAGTGCAGAGGAATTTGTAGAAGATGGATGGAAATTAGAAGATACAATAAAGCTTGTCAAGATATTAGAG
GGAAAAGGAGTAGCAGCTATTGATGTATCTTTAGGCACTATGTGTGAGGCTGCACCTATCACCATGCAGACATTGGGCAC
TAAAAAGGGCCTTTCATGGGATTATGCTGAAAAGATAAAGAGAGAAGTAAATATACCTGTAATAGCTGTTGGAAGGATAA
ATAGCCCTGATATTGCAGAAGAGATACTTTCAAAAGGTAAGGCTGACCTTGTGGCTTTAGGCAGGCCTTTAGTTGCTGAC
CCTCTATTCCTTGACAAGGCCGTTTCAGGGAGATTTAAGGAGATACGCCGCTGCCTTGCGTGCCATCAGGGTTGTTTTGA
CGAGCTAAGAGGAGGAAGAAGGTTTGGCTGCAACATAAACCCGGCTGTTGGAAGGAGCGACGAGATTGAGATACCTAAGG
CTGAAAATCCTGGCAAGAAGGTTTTCATAATAGGTGCTGGGCCTGCTGGGCTTGAAGCAGCTTATACTGCTGCTGTAAGA
GGGCATAAAGTAACTATTTATGACGAAAATGAAGAATTAGGCGGGCAACTCATCGCAGCTTCAATGCCACCTTATAAGGA
AGAACTCATGGGAATTGTAGAGTACTACAAGGAAGTGCTCCCTAAATTAGGAGTTGAGATAAAGCTAAATCACAGAGTGA
CTGCTGAAGAAGTAGCTCAGTGGGAAGCAGACGCAATAATATTTGCAACAGGAGGAGAGCCTATAGTTCTTTCAATACCT
GGGCTTAAAGAGTATGGATACATGACTGCTGCAGAAGTGCTTAAGGAAGGAAAAGAGACGGGGCAGACTGTCGTTATAAT
CGGTGGCGGCTTGATAGGTATGGAGACAGCGGACTACCTTTCCGAAAAAGGCAAAAAAGTCATGGTGATAGAGCTTTTAG
ATGAAGTAGCAAGAGATATGGGGGTATTTGAGAAAAATCTTTTGATGAAGAGGTTAAAGGAAAGAGATGTAGAAATTTAC
GTAAAGACTAAGCTCAAGGAAGTGAACAAGTCACTTGTAACAGAGACACTGGAAGGAGAAAGGAATCTTCCTCTTCCTGA
TACAATAGTTTTATCAGTCGGTATGAGGCCCAACAGGACTCTTTATGAGGAAGTAAAAAAGTTTGTGCCTGAGGAGAAGC
TCTATTTGATAGGGGATGCAAAAGAAGTGAGAAAAATTGTTCACGCTATCTCTGAAGGCCGGAATGTTGCGCTCACAATA
TGA

Upstream 100 bases:

>100_bases
CTTTGATAGAGAAAAAAACAAGAAGGAATTGTCTTTTTTGTGTCGAATAAATAAATTATGAACATTATTTTAAAACTCAT
AGCAGAGAAGGAGTGAGAAT

Downstream 100 bases:

>100_bases
GGGGATTTTCCCCTCTTTTTCAATTAAGGAGTTATCCACAGGGTGTTGTGAATATGTTAATAACTTGCTGTTAGAAATCT
AAAGATTGTAGTAAGTGAGG

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MELTKPIKLGGIELKNRLVMAPVKTGYATVEGQVTEELIGYYLNRAKGGVGLIVLESAYVDPSGKEIPRQLGIYDDSLVD
GLKRLVTPLQEAGSKVAVYINHAGRVANPMATKMPLIAPSAVMCPSIGVEPKAIEKEEIKKWQKAYVDAAIRAYKAGADF
LEIQFGHGYLIHQFLSPHANKRQDEYGGSFENRIRFGIEILEEIRSELPGFPVIVRISAEEFVEDGWKLEDTIKLVKILE
GKGVAAIDVSLGTMCEAAPITMQTLGTKKGLSWDYAEKIKREVNIPVIAVGRINSPDIAEEILSKGKADLVALGRPLVAD
PLFLDKAVSGRFKEIRRCLACHQGCFDELRGGRRFGCNINPAVGRSDEIEIPKAENPGKKVFIIGAGPAGLEAAYTAAVR
GHKVTIYDENEELGGQLIAASMPPYKEELMGIVEYYKEVLPKLGVEIKLNHRVTAEEVAQWEADAIIFATGGEPIVLSIP
GLKEYGYMTAAEVLKEGKETGQTVVIIGGGLIGMETADYLSEKGKKVMVIELLDEVARDMGVFEKNLLMKRLKERDVEIY
VKTKLKEVNKSLVTETLEGERNLPLPDTIVLSVGMRPNRTLYEEVKKFVPEEKLYLIGDAKEVRKIVHAISEGRNVALTI

Sequences:

>Translated_640_residues
MELTKPIKLGGIELKNRLVMAPVKTGYATVEGQVTEELIGYYLNRAKGGVGLIVLESAYVDPSGKEIPRQLGIYDDSLVD
GLKRLVTPLQEAGSKVAVYINHAGRVANPMATKMPLIAPSAVMCPSIGVEPKAIEKEEIKKWQKAYVDAAIRAYKAGADF
LEIQFGHGYLIHQFLSPHANKRQDEYGGSFENRIRFGIEILEEIRSELPGFPVIVRISAEEFVEDGWKLEDTIKLVKILE
GKGVAAIDVSLGTMCEAAPITMQTLGTKKGLSWDYAEKIKREVNIPVIAVGRINSPDIAEEILSKGKADLVALGRPLVAD
PLFLDKAVSGRFKEIRRCLACHQGCFDELRGGRRFGCNINPAVGRSDEIEIPKAENPGKKVFIIGAGPAGLEAAYTAAVR
GHKVTIYDENEELGGQLIAASMPPYKEELMGIVEYYKEVLPKLGVEIKLNHRVTAEEVAQWEADAIIFATGGEPIVLSIP
GLKEYGYMTAAEVLKEGKETGQTVVIIGGGLIGMETADYLSEKGKKVMVIELLDEVARDMGVFEKNLLMKRLKERDVEIY
VKTKLKEVNKSLVTETLEGERNLPLPDTIVLSVGMRPNRTLYEEVKKFVPEEKLYLIGDAKEVRKIVHAISEGRNVALTI
>Mature_640_residues
MELTKPIKLGGIELKNRLVMAPVKTGYATVEGQVTEELIGYYLNRAKGGVGLIVLESAYVDPSGKEIPRQLGIYDDSLVD
GLKRLVTPLQEAGSKVAVYINHAGRVANPMATKMPLIAPSAVMCPSIGVEPKAIEKEEIKKWQKAYVDAAIRAYKAGADF
LEIQFGHGYLIHQFLSPHANKRQDEYGGSFENRIRFGIEILEEIRSELPGFPVIVRISAEEFVEDGWKLEDTIKLVKILE
GKGVAAIDVSLGTMCEAAPITMQTLGTKKGLSWDYAEKIKREVNIPVIAVGRINSPDIAEEILSKGKADLVALGRPLVAD
PLFLDKAVSGRFKEIRRCLACHQGCFDELRGGRRFGCNINPAVGRSDEIEIPKAENPGKKVFIIGAGPAGLEAAYTAAVR
GHKVTIYDENEELGGQLIAASMPPYKEELMGIVEYYKEVLPKLGVEIKLNHRVTAEEVAQWEADAIIFATGGEPIVLSIP
GLKEYGYMTAAEVLKEGKETGQTVVIIGGGLIGMETADYLSEKGKKVMVIELLDEVARDMGVFEKNLLMKRLKERDVEIY
VKTKLKEVNKSLVTETLEGERNLPLPDTIVLSVGMRPNRTLYEEVKKFVPEEKLYLIGDAKEVRKIVHAISEGRNVALTI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=680, Percent_Identity=30.5882352941176, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787939, Length=342, Percent_Identity=28.9473684210526, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1789065, Length=213, Percent_Identity=25.3521126760563, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17564188, Length=345, Percent_Identity=29.8550724637681, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=345, Percent_Identity=29.8550724637681, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI72001454, Length=343, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17565138, Length=304, Percent_Identity=28.2894736842105, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17540738, Length=342, Percent_Identity=28.3625730994152, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=342, Percent_Identity=28.0701754385965, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17566914, Length=358, Percent_Identity=27.9329608938547, Blast_Score=105, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6325086, Length=217, Percent_Identity=30.8755760368664, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321973, Length=216, Percent_Identity=31.4814814814815, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 70382; Mature: 70382

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS51007 CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTKPIKLGGIELKNRLVMAPVKTGYATVEGQVTEELIGYYLNRAKGGVGLIVLESAYV
CCCCCCCEECCEEECCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEC
DPSGKEIPRQLGIYDDSLVDGLKRLVTPLQEAGSKVAVYINHAGRVANPMATKMPLIAPS
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCCCC
AVMCPSIGVEPKAIEKEEIKKWQKAYVDAAIRAYKAGADFLEIQFGHGYLIHQFLSPHAN
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCC
KRQDEYGGSFENRIRFGIEILEEIRSELPGFPVIVRISAEEFVEDGWKLEDTIKLVKILE
CCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHC
GKGVAAIDVSLGTMCEAAPITMQTLGTKKGLSWDYAEKIKREVNIPVIAVGRINSPDIAE
CCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
EILSKGKADLVALGRPLVADPLFLDKAVSGRFKEIRRCLACHQGCFDELRGGRRFGCNIN
HHHHCCCCCEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCCCC
PAVGRSDEIEIPKAENPGKKVFIIGAGPAGLEAAYTAAVRGHKVTIYDENEELGGQLIAA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEC
SMPPYKEELMGIVEYYKEVLPKLGVEIKLNHRVTAEEVAQWEADAIIFATGGEPIVLSIP
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCEEEEEECCCEEEEECC
GLKEYGYMTAAEVLKEGKETGQTVVIIGGGLIGMETADYLSEKGKKVMVIELLDEVARDM
CCHHHCHHHHHHHHHHHHHCCCEEEEECCCEECCCHHHHHHHCCCEEEEHHHHHHHHHHH
GVFEKNLLMKRLKERDVEIYVKTKLKEVNKSLVTETLEGERNLPLPDTIVLSVGMRPNRT
HHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHH
LYEEVKKFVPEEKLYLIGDAKEVRKIVHAISEGRNVALTI
HHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MELTKPIKLGGIELKNRLVMAPVKTGYATVEGQVTEELIGYYLNRAKGGVGLIVLESAYV
CCCCCCCEECCEEECCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEEEEC
DPSGKEIPRQLGIYDDSLVDGLKRLVTPLQEAGSKVAVYINHAGRVANPMATKMPLIAPS
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCCCC
AVMCPSIGVEPKAIEKEEIKKWQKAYVDAAIRAYKAGADFLEIQFGHGYLIHQFLSPHAN
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCC
KRQDEYGGSFENRIRFGIEILEEIRSELPGFPVIVRISAEEFVEDGWKLEDTIKLVKILE
CCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHC
GKGVAAIDVSLGTMCEAAPITMQTLGTKKGLSWDYAEKIKREVNIPVIAVGRINSPDIAE
CCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
EILSKGKADLVALGRPLVADPLFLDKAVSGRFKEIRRCLACHQGCFDELRGGRRFGCNIN
HHHHCCCCCEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCCCC
PAVGRSDEIEIPKAENPGKKVFIIGAGPAGLEAAYTAAVRGHKVTIYDENEELGGQLIAA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEC
SMPPYKEELMGIVEYYKEVLPKLGVEIKLNHRVTAEEVAQWEADAIIFATGGEPIVLSIP
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCEEEEEECCCEEEEECC
GLKEYGYMTAAEVLKEGKETGQTVVIIGGGLIGMETADYLSEKGKKVMVIELLDEVARDM
CCHHHCHHHHHHHHHHHHHCCCEEEEECCCEECCCHHHHHHHCCCEEEEHHHHHHHHHHH
GVFEKNLLMKRLKERDVEIYVKTKLKEVNKSLVTETLEGERNLPLPDTIVLSVGMRPNRT
HHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHH
LYEEVKKFVPEEKLYLIGDAKEVRKIVHAISEGRNVALTI
HHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]