Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 20808954

GI number: 20808954

Start: 2495686

End: 2497299

Strand: Reverse

Name: pyrG

Synonym: TTE2609

Alternate gene names: 20808954

Gene position: 2497299-2495686 (Counterclockwise)

Preceding gene: 20808956

Following gene: 20808953

Centisome position: 92.86

GC content: 40.46

Gene sequence:

>1614_bases
ATGGCAAAGTATATTTTTGTAACAGGAGGAGTAGTCTCCTCTTTAGGTAAAGGCATAACTGCTGCATCATTGGGAAGGCT
ACTAAAAAGCCGCGGGCTTTCTGTTGCTATGCAGAAATTTGACCCGTATATAAACATAGACCCTGGGACAATGAGCCCTT
ATCAGCATGGAGAAGTATTTGTAACAGAGGACGGAGCAGAAACGGACCTGGATTTGGGGCATTATGAAAGATTCATAGAT
GTAAATCTGACTAAAAACAGCAATGTCACTGCGGGCAAAATATACTGGTCTGTTATCACAAAAGAAAGAAAAGGAGATTA
TCTCGGCGCTACTGTTCAGGTAATACCCCACATTACTGATGAAATAAAAGAAAGAGTGTACAGAGTAGCAAAAGAAAAAA
ATGTAGATGTGGTTATAACAGAAATTGGTGGAACAGTAGGAGACATAGAAAGCCTTCCTTTCTTGGAGGCAATAAGGCAG
GTGGCTATTGAACAGGGAAGAGAAAATGTTATGTTCATTCACGTAACGCTTGTTCCACATCTGGGCAACACAGGTGAACT
GAAAACAAAGCCTACACAGCACAGCGTAAAAGAATTAAGGTCCATAGGTATTCAGCCGGACATGATAGTCTGTAGAACTG
AGCTTCCACTTCCTCAGGAATTGAGAGAAAAAATTGCTCTATTCTGCAATGTAAGTGTAGAAGCGGTCATTGAGAATAGA
GATGTAGAATCGATCTATCAAGTTCCTCTGGAACTTGAAAGGCAGAAAGTAGACGAATATGTGATAAAAAGGCTGAATTT
ACCCCTTGGGCAGTCTGATTTAAAAGAGTGGAGAGAATACGTTGAAAAAGAGAAAAATCCTGAACATCAGGTAGAGGTAG
CACTTGTAGGCAAATATGTCGACCTACATGATGCCTATATAAGCGTTGTAGAAGCTTTAAAACATGCAGGGGTTTATCAC
AAAACAGCTGTCAACATCCGATGGGTTAATGCAGAAAAAGTAAATGACAAAACAGTTGATGAACTTTTAAAAGGTGCAGA
TGGAATACTTGTTCCCGGCGGCTTTGGCGACAGAGGAATAGAAGGAAAGATAAGGGCAATTCAGTACGCAAGGGAAAATA
AGATTCCCTATCTGGGGCTGTGTCTTGGAATGCAGTGTGCGGTCATAGAATTTGCGAGAAATGTGGCAGGGCTTAAAGGG
GCCAATTCCACCGAATTTGACCCCAATACCCCTCATCCAGTAATTGACCTCATGCCCGAACAAAAAGACATAGATGAAAA
AGGCGGCACAATGAGACTGGGAGTATACCCTTGTAAAGTCATTGAAGGAACAAAGGCATATGAAGTATACAAAGATGAGC
TAGTTTATGAAAGGCACAGGCACAGGTATGAATTTAACAACCAGTACAGAGAGCTTTTAACCTCAAAAGGGCTTGTAATC
TCAGGCCTTTCTCCAGATGAGAGGCTTGTTGAAATAATAGAGTTAAAAGACCATCCTTATTTTGTAGCAACCCAATTTCA
TCCAGAATTCAAATCAAGACCTTTAAACCCACACCCTCTTTTCAGAGATTTTGTAAAGGCAATGCTTAACTTAAAAATAA
ATAATGCAGAGTAA

Upstream 100 bases:

>100_bases
CAAGTCTCTATTTTTCTCAAAAATAGAGATTTTTTTATGTGTTAAAAGAGCTAAAAAATTATTATCAAAAAACGATATAC
TCATCAAGAGGTGAAAAAAG

Downstream 100 bases:

>100_bases
AGGCTGGACTCCCCAGCCTTTAAATTTTCTTTGAATAATAAAGTTAAAAACGATATAATACATTTAGAGATTAATTTTAA
GATTAATCTATGAGGTGGTG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MAKYIFVTGGVVSSLGKGITAASLGRLLKSRGLSVAMQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFID
VNLTKNSNVTAGKIYWSVITKERKGDYLGATVQVIPHITDEIKERVYRVAKEKNVDVVITEIGGTVGDIESLPFLEAIRQ
VAIEQGRENVMFIHVTLVPHLGNTGELKTKPTQHSVKELRSIGIQPDMIVCRTELPLPQELREKIALFCNVSVEAVIENR
DVESIYQVPLELERQKVDEYVIKRLNLPLGQSDLKEWREYVEKEKNPEHQVEVALVGKYVDLHDAYISVVEALKHAGVYH
KTAVNIRWVNAEKVNDKTVDELLKGADGILVPGGFGDRGIEGKIRAIQYARENKIPYLGLCLGMQCAVIEFARNVAGLKG
ANSTEFDPNTPHPVIDLMPEQKDIDEKGGTMRLGVYPCKVIEGTKAYEVYKDELVYERHRHRYEFNNQYRELLTSKGLVI
SGLSPDERLVEIIELKDHPYFVATQFHPEFKSRPLNPHPLFRDFVKAMLNLKINNAE

Sequences:

>Translated_537_residues
MAKYIFVTGGVVSSLGKGITAASLGRLLKSRGLSVAMQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFID
VNLTKNSNVTAGKIYWSVITKERKGDYLGATVQVIPHITDEIKERVYRVAKEKNVDVVITEIGGTVGDIESLPFLEAIRQ
VAIEQGRENVMFIHVTLVPHLGNTGELKTKPTQHSVKELRSIGIQPDMIVCRTELPLPQELREKIALFCNVSVEAVIENR
DVESIYQVPLELERQKVDEYVIKRLNLPLGQSDLKEWREYVEKEKNPEHQVEVALVGKYVDLHDAYISVVEALKHAGVYH
KTAVNIRWVNAEKVNDKTVDELLKGADGILVPGGFGDRGIEGKIRAIQYARENKIPYLGLCLGMQCAVIEFARNVAGLKG
ANSTEFDPNTPHPVIDLMPEQKDIDEKGGTMRLGVYPCKVIEGTKAYEVYKDELVYERHRHRYEFNNQYRELLTSKGLVI
SGLSPDERLVEIIELKDHPYFVATQFHPEFKSRPLNPHPLFRDFVKAMLNLKINNAE
>Mature_536_residues
AKYIFVTGGVVSSLGKGITAASLGRLLKSRGLSVAMQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDV
NLTKNSNVTAGKIYWSVITKERKGDYLGATVQVIPHITDEIKERVYRVAKEKNVDVVITEIGGTVGDIESLPFLEAIRQV
AIEQGRENVMFIHVTLVPHLGNTGELKTKPTQHSVKELRSIGIQPDMIVCRTELPLPQELREKIALFCNVSVEAVIENRD
VESIYQVPLELERQKVDEYVIKRLNLPLGQSDLKEWREYVEKEKNPEHQVEVALVGKYVDLHDAYISVVEALKHAGVYHK
TAVNIRWVNAEKVNDKTVDELLKGADGILVPGGFGDRGIEGKIRAIQYARENKIPYLGLCLGMQCAVIEFARNVAGLKGA
NSTEFDPNTPHPVIDLMPEQKDIDEKGGTMRLGVYPCKVIEGTKAYEVYKDELVYERHRHRYEFNNQYRELLTSKGLVIS
GLSPDERLVEIIELKDHPYFVATQFHPEFKSRPLNPHPLFRDFVKAMLNLKINNAE

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=542, Percent_Identity=50.9225092250922, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=49.908592321755, Blast_Score=517, Evalue=1e-147,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=49.908592321755, Blast_Score=517, Evalue=1e-147,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=49.908592321755, Blast_Score=517, Evalue=1e-147,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=56.7164179104478, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI25148299, Length=595, Percent_Identity=42.3529411764706, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6319432, Length=557, Percent_Identity=46.8581687612208, Blast_Score=482, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=47.2271914132379, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=50.4553734061931, Blast_Score=520, Evalue=1e-148,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=48.582995951417, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_THETN (Q8R720)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624125.1
- ProteinModelPortal:   Q8R720
- SMR:   Q8R720
- GeneID:   997261
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2609
- NMPDR:   fig|273068.3.peg.2117
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   TTEN273068:TTE2609-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60602; Mature: 60471

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYIFVTGGVVSSLGKGITAASLGRLLKSRGLSVAMQKFDPYINIDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHCCCEEEECCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFIDVNLTKNSNVTAGKIYWSVITKERKGDYLGATVQVIPHITD
EECCCCCCCCCCCCCEEEEEEEEECCCCCEEHEEEEEEEEHHCCCCCCCHHHHHHHHHHH
EIKERVYRVAKEKNVDVVITEIGGTVGDIESLPFLEAIRQVAIEQGRENVMFIHVTLVPH
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEC
LGNTGELKTKPTQHSVKELRSIGIQPDMIVCRTELPLPQELREKIALFCNVSVEAVIENR
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCC
DVESIYQVPLELERQKVDEYVIKRLNLPLGQSDLKEWREYVEKEKNPEHQVEVALVGKYV
CHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECHH
DLHDAYISVVEALKHAGVYHKTAVNIRWVNAEKVNDKTVDELLKGADGILVPGGFGDRGI
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
EGKIRAIQYARENKIPYLGLCLGMQCAVIEFARNVAGLKGANSTEFDPNTPHPVIDLMPE
CCHHHHEEEHHHCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCC
QKDIDEKGGTMRLGVYPCKVIEGTKAYEVYKDELVYERHRHRYEFNNQYRELLTSKGLVI
CCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEE
SGLSPDERLVEIIELKDHPYFVATQFHPEFKSRPLNPHPLFRDFVKAMLNLKINNAE
ECCCCHHHHHHHHEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHCCEECCCC
>Mature Secondary Structure 
AKYIFVTGGVVSSLGKGITAASLGRLLKSRGLSVAMQKFDPYINIDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHCCCEEEECCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFIDVNLTKNSNVTAGKIYWSVITKERKGDYLGATVQVIPHITD
EECCCCCCCCCCCCCEEEEEEEEECCCCCEEHEEEEEEEEHHCCCCCCCHHHHHHHHHHH
EIKERVYRVAKEKNVDVVITEIGGTVGDIESLPFLEAIRQVAIEQGRENVMFIHVTLVPH
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEC
LGNTGELKTKPTQHSVKELRSIGIQPDMIVCRTELPLPQELREKIALFCNVSVEAVIENR
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCC
DVESIYQVPLELERQKVDEYVIKRLNLPLGQSDLKEWREYVEKEKNPEHQVEVALVGKYV
CHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECHH
DLHDAYISVVEALKHAGVYHKTAVNIRWVNAEKVNDKTVDELLKGADGILVPGGFGDRGI
HHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
EGKIRAIQYARENKIPYLGLCLGMQCAVIEFARNVAGLKGANSTEFDPNTPHPVIDLMPE
CCHHHHEEEHHHCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCC
QKDIDEKGGTMRLGVYPCKVIEGTKAYEVYKDELVYERHRHRYEFNNQYRELLTSKGLVI
CCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEE
SGLSPDERLVEIIELKDHPYFVATQFHPEFKSRPLNPHPLFRDFVKAMLNLKINNAE
ECCCCHHHHHHHHEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336