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The map label for this gene is sleB [H]

Identifier: 20808900

GI number: 20808900

Start: 2440032

End: 2440736

Strand: Reverse

Name: sleB [H]

Synonym: TTE2551

Alternate gene names: 20808900

Gene position: 2440736-2440032 (Counterclockwise)

Preceding gene: 20808901

Following gene: 20808899

Centisome position: 90.75

GC content: 45.53

Gene sequence:

>705_bases
TTGAGCTTTAGAGTGCGCTTTAAGGTTATAATAGCCATTTTGGTCGTTTTTACGACAGCCGTCTTTGAGTATACAGCTAT
AAGCATGACCAAAACTGCTATGTCCAATTTGTACTGGGGAAACACAGGAAGTGATGTTGCTAAAGTGCAGGCAAGGCTTA
AAGCCTGGGGATATTACGACGGCCCGGTGGACGGTTTTTTTGGCGTTAAGACATGGCTTGCTGTTAGAAAATTTCAGGCC
TATAACGGCCTTGCAGTGACAGGCATTGTTGATGACGATACAAAAGTGGCATTGGGGTTTACGACTACTGCACAGGATTT
GGCAGCCTATAGAGCTACTTCTTCTGCAGGCATAAGTGATGATGTCTATCTTCTGGCAATGCTCATAAATGGTGAAGCGA
GAGGAGAACCCTACATAGGAAAGGTCGCTGTTGGGGCTGTTGTAATGAATAGGGTAAGAGACCCGAGATTTCCCAAAACT
ATTCCAGGAGTAATTTTTCAGCCTGGGGCTTTTTCTGCTGTAGAAGACGGGCAGATGTGGCTTCCTCCTACTGAGGAAAG
CATAAAAGCAGCAATAGATGCCGTATCCGGCTGGGACCCGACAGGAGGAGCTCTCTATTACTACAATGCAGCGAGAGTGA
CGAATTACTGGATATTTACAAGACCCATATTGACTCAGATAGGACGTCACATATTTGCAAGGTGA

Upstream 100 bases:

>100_bases
GCCTTAAAGGCCTTGAAAAGGGCCTTTTATTTTTTAAGTGAATAATAGTTTTATTACTACAAAAAATATTTACAGACGCA
AGAAAGGAGGCAAGAGAAAA

Downstream 100 bases:

>100_bases
GGTGGAGATTATGTTAAAACGATGGTTCACAGCTTTTGTGCTGATTTTGGTTTTAGCTTTGGGAGCCTGGGGCTACAGGC
AATATTTAGAAAAAATTTCT

Product: spore cortex-lytic enzyme prepeptide

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSFRVRFKVIIAILVVFTTAVFEYTAISMTKTAMSNLYWGNTGSDVAKVQARLKAWGYYDGPVDGFFGVKTWLAVRKFQA
YNGLAVTGIVDDDTKVALGFTTTAQDLAAYRATSSAGISDDVYLLAMLINGEARGEPYIGKVAVGAVVMNRVRDPRFPKT
IPGVIFQPGAFSAVEDGQMWLPPTEESIKAAIDAVSGWDPTGGALYYYNAARVTNYWIFTRPILTQIGRHIFAR

Sequences:

>Translated_234_residues
MSFRVRFKVIIAILVVFTTAVFEYTAISMTKTAMSNLYWGNTGSDVAKVQARLKAWGYYDGPVDGFFGVKTWLAVRKFQA
YNGLAVTGIVDDDTKVALGFTTTAQDLAAYRATSSAGISDDVYLLAMLINGEARGEPYIGKVAVGAVVMNRVRDPRFPKT
IPGVIFQPGAFSAVEDGQMWLPPTEESIKAAIDAVSGWDPTGGALYYYNAARVTNYWIFTRPILTQIGRHIFAR
>Mature_233_residues
SFRVRFKVIIAILVVFTTAVFEYTAISMTKTAMSNLYWGNTGSDVAKVQARLKAWGYYDGPVDGFFGVKTWLAVRKFQAY
NGLAVTGIVDDDTKVALGFTTTAQDLAAYRATSSAGISDDVYLLAMLINGEARGEPYIGKVAVGAVVMNRVRDPRFPKTI
PGVIFQPGAFSAVEDGQMWLPPTEESIKAAIDAVSGWDPTGGALYYYNAARVTNYWIFTRPILTQIGRHIFAR

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 25666; Mature: 25535

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRVRFKVIIAILVVFTTAVFEYTAISMTKTAMSNLYWGNTGSDVAKVQARLKAWGYYD
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
GPVDGFFGVKTWLAVRKFQAYNGLAVTGIVDDDTKVALGFTTTAQDLAAYRATSSAGISD
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCC
DVYLLAMLINGEARGEPYIGKVAVGAVVMNRVRDPRFPKTIPGVIFQPGAFSAVEDGQMW
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCEEE
LPPTEESIKAAIDAVSGWDPTGGALYYYNAARVTNYWIFTRPILTQIGRHIFAR
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHEEEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFRVRFKVIIAILVVFTTAVFEYTAISMTKTAMSNLYWGNTGSDVAKVQARLKAWGYYD
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
GPVDGFFGVKTWLAVRKFQAYNGLAVTGIVDDDTKVALGFTTTAQDLAAYRATSSAGISD
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCC
DVYLLAMLINGEARGEPYIGKVAVGAVVMNRVRDPRFPKTIPGVIFQPGAFSAVEDGQMW
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCEEE
LPPTEESIKAAIDAVSGWDPTGGALYYYNAARVTNYWIFTRPILTQIGRHIFAR
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHEEEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]