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The map label for this gene is OppA10 [H]

Identifier: 20808876

GI number: 20808876

Start: 2412450

End: 2414210

Strand: Direct

Name: OppA10 [H]

Synonym: TTE2522

Alternate gene names: 20808876

Gene position: 2412450-2414210 (Clockwise)

Preceding gene: 20808872

Following gene: 20808877

Centisome position: 89.7

GC content: 41.74

Gene sequence:

>1761_bases
TTGAAGATGCTAAGAAAGAAATGGCTTCCTCTTTTAGTGGTGCTCGTCTTCGCCCTATCAGTTGTCCTATCTGCTTGCAG
CAAACCACAGGAGACACAACCATCCGAACAGCCCAAGCAAGAACAAACAACTCCAACAGCTTCTGTCCAGGACAGCGACA
ACTGGACAAAAGCAAAAGACCCTTCAAAAATACCAGAAGCTTCAAAATCTCGTACAGACACTCTCATAATAGGTCTGCAT
GAGCCAGCAGGAACATTTAACCCACTCTTCTATGAGACAGCCTATGACAATTACGTCTTAAGCGCTATATTTGATGGCTT
AATAGAAGTAAACTTTGACGGAACATATATACCTGGCCTTGCAGAGAAGTGGGATTTGGACAAGGACAATAACACTGTTA
CGTTCCACTTAAGAAAAGGGGTACAATTCAGTGACGGTACACCTTTAACAGCCCATGACGTAGAATTTACTTACTACGTA
TTAGCAGACCCAACTTACGATGGACCAATTGACCTCTCTCCTGCAGGTATAATAGGATTTAAGGAGTATCAGGAGGGCAA
AGCAGATAAAATTGAAGGTATAAAGGTCTTAGATGACTACACAATACAAATTCAATTAGAAAAACTCATGGGAACTAGCT
TGTCAGATTTGGGTATACCTGTAATTCCAAAGCACTACTATGGAAAAGACTTTAAGAAGGGAAATCTTGAGGGCATAAAG
GCTTTAATGCAAAAGCCACTTGGTTCGGGTCCATATGTATTTGACAAATTTGTACCTGGTCAGGAAGTGCGCTTAGTAGC
AAATGAGCACTACTGGAAAGGTGCTCCAAAAATAAAGAATCTCATCTTCAAAACGACTACAGACGAAACAGCGGTTCAGA
TGTTGCAGGCAGGAGAAACAGACTATGAAACTTCCAACATCACAGCTAAAAAGGACAATGTAGAGCTCCTCGAAAGCCTG
GGATTTGTAGACTATGCACTGTTGCCCACAAACGGATATGGGTATATCGCTTTTAACTTCTATCAGCCAAAATTCCAGGA
TGTAAGAGTTCGCCAGGCTCTGACATACGGTCTTAACAGAAAAGCTATAGTTGATGCAGTATATCAGGGGCTAGCAGAAG
TGAGAAACGTTCCTCAGTCAAAAGTGTCCTGGGCATATCCGCCGGAAGATGAAATAAATCCATATAACTACGATCCAGAA
AAGGCAAAACAGCTCCTTGAAGAGGCAGGCTGGAAGCTAGGACCTGACGGGTATCGCTATAAGAATGGTGAAAAGTTCAC
AATTCACTTCCTGCAGTCAACACCAAACCCTGTGAACGACGCATTAGTGCCAATCGCTGTTGAAAACTATAAACAATTAG
GTATTGAGTTTATAGCAGAACCCATGGAGTTTAACTTAATAGTTGATAAAATTAAGAAAGCCAAAGAAAAGACTTTTGAC
ATGTACTTCATGGCTTGGGGATTAAGTGCTGAACCAGATCCATACAACATATTCCACTCCAAAGGGTCACAGAACAGAAA
CGGATACTCCAATCCAAAGGTCGATGAGCTTATTGTAAAAGCTCGCGAAGAGCTAGATATGAATAAGCGCAAAGAGCTCT
ACCATGAGCTTTACAAAGTGCTCAATGAAGACCTGCCGTACATCTTCATGTACCAGAGACATGATCTTAATGCCAAGAAC
ATGAGAGTAGTAGGATTCCAGATTTCATCCTTCAGAGACTTCACTTACTCGCTCTATCAGGCAGAACTTCTCCAGTATTA
A

Upstream 100 bases:

>100_bases
TGTTTTACATTTTTAACAGCCATATTGCCAACTAATATTAAATATGCTATAATAGTAATCGTCTACAAAATAAAAACAAA
ATTAAAGGTAAGGGGGATTT

Downstream 100 bases:

>100_bases
ATTAAGTAAGAAAAGCCCGGACTTAGGGCCGGGCTTTTCCAATGCATTTTAGGAGGGGATTTGATGACACAGTTTATAAT
AAGAAGGCTTTTGCAGAGCA

Product: ABC-type dipeptide/oligopeptide/nickel transport systems, periplasmic components

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKMLRKKWLPLLVVLVFALSVVLSACSKPQETQPSEQPKQEQTTPTASVQDSDNWTKAKDPSKIPEASKSRTDTLIIGLH
EPAGTFNPLFYETAYDNYVLSAIFDGLIEVNFDGTYIPGLAEKWDLDKDNNTVTFHLRKGVQFSDGTPLTAHDVEFTYYV
LADPTYDGPIDLSPAGIIGFKEYQEGKADKIEGIKVLDDYTIQIQLEKLMGTSLSDLGIPVIPKHYYGKDFKKGNLEGIK
ALMQKPLGSGPYVFDKFVPGQEVRLVANEHYWKGAPKIKNLIFKTTTDETAVQMLQAGETDYETSNITAKKDNVELLESL
GFVDYALLPTNGYGYIAFNFYQPKFQDVRVRQALTYGLNRKAIVDAVYQGLAEVRNVPQSKVSWAYPPEDEINPYNYDPE
KAKQLLEEAGWKLGPDGYRYKNGEKFTIHFLQSTPNPVNDALVPIAVENYKQLGIEFIAEPMEFNLIVDKIKKAKEKTFD
MYFMAWGLSAEPDPYNIFHSKGSQNRNGYSNPKVDELIVKAREELDMNKRKELYHELYKVLNEDLPYIFMYQRHDLNAKN
MRVVGFQISSFRDFTYSLYQAELLQY

Sequences:

>Translated_586_residues
MKMLRKKWLPLLVVLVFALSVVLSACSKPQETQPSEQPKQEQTTPTASVQDSDNWTKAKDPSKIPEASKSRTDTLIIGLH
EPAGTFNPLFYETAYDNYVLSAIFDGLIEVNFDGTYIPGLAEKWDLDKDNNTVTFHLRKGVQFSDGTPLTAHDVEFTYYV
LADPTYDGPIDLSPAGIIGFKEYQEGKADKIEGIKVLDDYTIQIQLEKLMGTSLSDLGIPVIPKHYYGKDFKKGNLEGIK
ALMQKPLGSGPYVFDKFVPGQEVRLVANEHYWKGAPKIKNLIFKTTTDETAVQMLQAGETDYETSNITAKKDNVELLESL
GFVDYALLPTNGYGYIAFNFYQPKFQDVRVRQALTYGLNRKAIVDAVYQGLAEVRNVPQSKVSWAYPPEDEINPYNYDPE
KAKQLLEEAGWKLGPDGYRYKNGEKFTIHFLQSTPNPVNDALVPIAVENYKQLGIEFIAEPMEFNLIVDKIKKAKEKTFD
MYFMAWGLSAEPDPYNIFHSKGSQNRNGYSNPKVDELIVKAREELDMNKRKELYHELYKVLNEDLPYIFMYQRHDLNAKN
MRVVGFQISSFRDFTYSLYQAELLQY
>Mature_586_residues
MKMLRKKWLPLLVVLVFALSVVLSACSKPQETQPSEQPKQEQTTPTASVQDSDNWTKAKDPSKIPEASKSRTDTLIIGLH
EPAGTFNPLFYETAYDNYVLSAIFDGLIEVNFDGTYIPGLAEKWDLDKDNNTVTFHLRKGVQFSDGTPLTAHDVEFTYYV
LADPTYDGPIDLSPAGIIGFKEYQEGKADKIEGIKVLDDYTIQIQLEKLMGTSLSDLGIPVIPKHYYGKDFKKGNLEGIK
ALMQKPLGSGPYVFDKFVPGQEVRLVANEHYWKGAPKIKNLIFKTTTDETAVQMLQAGETDYETSNITAKKDNVELLESL
GFVDYALLPTNGYGYIAFNFYQPKFQDVRVRQALTYGLNRKAIVDAVYQGLAEVRNVPQSKVSWAYPPEDEINPYNYDPE
KAKQLLEEAGWKLGPDGYRYKNGEKFTIHFLQSTPNPVNDALVPIAVENYKQLGIEFIAEPMEFNLIVDKIKKAKEKTFD
MYFMAWGLSAEPDPYNIFHSKGSQNRNGYSNPKVDELIVKAREELDMNKRKELYHELYKVLNEDLPYIFMYQRHDLNAKN
MRVVGFQISSFRDFTYSLYQAELLQY

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=509, Percent_Identity=28.8801571709234, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789887, Length=491, Percent_Identity=26.0692464358452, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1787052, Length=523, Percent_Identity=24.282982791587, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081878, Length=496, Percent_Identity=26.8145161290323, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787495, Length=509, Percent_Identity=26.1296660117878, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789397, Length=521, Percent_Identity=24.5681381957774, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787551, Length=539, Percent_Identity=23.191094619666, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=23.3791748526523, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66821; Mature: 66821

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLRKKWLPLLVVLVFALSVVLSACSKPQETQPSEQPKQEQTTPTASVQDSDNWTKAKD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
PSKIPEASKSRTDTLIIGLHEPAGTFNPLFYETAYDNYVLSAIFDGLIEVNFDGTYIPGL
CCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCEEECCCCEECCCC
AEKWDLDKDNNTVTFHLRKGVQFSDGTPLTAHDVEFTYYVLADPTYDGPIDLSPAGIIGF
CHHCCCCCCCCEEEEEEECCCEECCCCCCEEECCEEEEEEEECCCCCCCCCCCCCCEEEC
KEYQEGKADKIEGIKVLDDYTIQIQLEKLMGTSLSDLGIPVIPKHYYGKDFKKGNLEGIK
HHHCCCCCCCCCCEEEEECEEEEEEEHHHHCCCHHHCCCCCCCCHHCCCCCCCCCHHHHH
ALMQKPLGSGPYVFDKFVPGQEVRLVANEHYWKGAPKIKNLIFKTTTDETAVQMLQAGET
HHHHCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHEEECCCHHHHHHHHHCCCC
DYETSNITAKKDNVELLESLGFVDYALLPTNGYGYIAFNFYQPKFQDVRVRQALTYGLNR
CCCCCCEEECCCCHHHHHHCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCH
KAIVDAVYQGLAEVRNVPQSKVSWAYPPEDEINPYNYDPEKAKQLLEEAGWKLGPDGYRY
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEE
KNGEKFTIHFLQSTPNPVNDALVPIAVENYKQLGIEFIAEPMEFNLIVDKIKKAKEKTFD
CCCCEEEEEEEECCCCCCCCCEEEEEHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHE
MYFMAWGLSAEPDPYNIFHSKGSQNRNGYSNPKVDELIVKAREELDMNKRKELYHELYKV
EEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
LNEDLPYIFMYQRHDLNAKNMRVVGFQISSFRDFTYSLYQAELLQY
HHCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKMLRKKWLPLLVVLVFALSVVLSACSKPQETQPSEQPKQEQTTPTASVQDSDNWTKAKD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
PSKIPEASKSRTDTLIIGLHEPAGTFNPLFYETAYDNYVLSAIFDGLIEVNFDGTYIPGL
CCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCEEECCCCEECCCC
AEKWDLDKDNNTVTFHLRKGVQFSDGTPLTAHDVEFTYYVLADPTYDGPIDLSPAGIIGF
CHHCCCCCCCCEEEEEEECCCEECCCCCCEEECCEEEEEEEECCCCCCCCCCCCCCEEEC
KEYQEGKADKIEGIKVLDDYTIQIQLEKLMGTSLSDLGIPVIPKHYYGKDFKKGNLEGIK
HHHCCCCCCCCCCEEEEECEEEEEEEHHHHCCCHHHCCCCCCCCHHCCCCCCCCCHHHHH
ALMQKPLGSGPYVFDKFVPGQEVRLVANEHYWKGAPKIKNLIFKTTTDETAVQMLQAGET
HHHHCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHEEECCCHHHHHHHHHCCCC
DYETSNITAKKDNVELLESLGFVDYALLPTNGYGYIAFNFYQPKFQDVRVRQALTYGLNR
CCCCCCEEECCCCHHHHHHCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCH
KAIVDAVYQGLAEVRNVPQSKVSWAYPPEDEINPYNYDPEKAKQLLEEAGWKLGPDGYRY
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEE
KNGEKFTIHFLQSTPNPVNDALVPIAVENYKQLGIEFIAEPMEFNLIVDKIKKAKEKTFD
CCCCEEEEEEEECCCCCCCCCEEEEEHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHE
MYFMAWGLSAEPDPYNIFHSKGSQNRNGYSNPKVDELIVKAREELDMNKRKELYHELYKV
EEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
LNEDLPYIFMYQRHDLNAKNMRVVGFQISSFRDFTYSLYQAELLQY
HHCCCCEEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]