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The map label for this gene is Ada [H]

Identifier: 20808873

GI number: 20808873

Start: 2409232

End: 2409771

Strand: Reverse

Name: Ada [H]

Synonym: TTE2519

Alternate gene names: 20808873

Gene position: 2409771-2409232 (Counterclockwise)

Preceding gene: 20808874

Following gene: 20808871

Centisome position: 89.6

GC content: 36.67

Gene sequence:

>540_bases
GTGATTGTGAAGTTTTTTCTGTGCAAGTTTACTTTTTACTTTAAAAATGATAAAGGGGAATTTTATATGATTTACAAGAG
ATTTAACTCTCCGGTAGGTGTTCTTACAGTATTTTCTTCTGGCAAAGGTATATGCAAAATTGAGTTTGAAAATGAAAAAT
TTCCATTAGAGGATTTTGAAGAGGGAACCGATTTTTATATTGAAGAGTGCATTAAAGAGCTTGACCTGTATTTTCAAAAG
AGGCTGAAGAATTTTGATGTGAAGCTGGATTTGCAAGGTACGAAGTTTCAGATGGCTGTGTGGGAGGAGACTTTGAAAAT
TCCCTATGGATCTGTTGTGACATACGGTGAAATTGCAAGAAGAATTGGGAAACCCAAAGCAGCGAGAGCAGTAGGGCAGG
CTCTTAATAAAAATTCTATTCCCATTATAATTCCCTGTCACAGAGTTATTGGAAGCGACGGAAACTTGACAGGTTTTGGA
GGGGGGATTGAGATAAAAAAGTTTTTACTTAAGCATGAAGGGGTTAAGATTGGTGGATGA

Upstream 100 bases:

>100_bases
ACTTTCTTTCCATTTTTTATCTCTCCCTGACGAAATCTCCCCAATATTATAGACGCGGAGTAACACTATTTGGTTTCATT
TTTATGTATTATTTTTTCTC

Downstream 100 bases:

>100_bases
TAAGAAGAAGCCAGAGGGAATTGGATAAGAGATCACAATGGCGCAATTGATAATAATTTTGTCAATTGTGCCATTATTTT
TCTTGACTTTGTCAGTTTGA

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MIVKFFLCKFTFYFKNDKGEFYMIYKRFNSPVGVLTVFSSGKGICKIEFENEKFPLEDFEEGTDFYIEECIKELDLYFQK
RLKNFDVKLDLQGTKFQMAVWEETLKIPYGSVVTYGEIARRIGKPKAARAVGQALNKNSIPIIIPCHRVIGSDGNLTGFG
GGIEIKKFLLKHEGVKIGG

Sequences:

>Translated_179_residues
MIVKFFLCKFTFYFKNDKGEFYMIYKRFNSPVGVLTVFSSGKGICKIEFENEKFPLEDFEEGTDFYIEECIKELDLYFQK
RLKNFDVKLDLQGTKFQMAVWEETLKIPYGSVVTYGEIARRIGKPKAARAVGQALNKNSIPIIIPCHRVIGSDGNLTGFG
GGIEIKKFLLKHEGVKIGG
>Mature_179_residues
MIVKFFLCKFTFYFKNDKGEFYMIYKRFNSPVGVLTVFSSGKGICKIEFENEKFPLEDFEEGTDFYIEECIKELDLYFQK
RLKNFDVKLDLQGTKFQMAVWEETLKIPYGSVVTYGEIARRIGKPKAARAVGQALNKNSIPIIIPCHRVIGSDGNLTGFG
GGIEIKKFLLKHEGVKIGG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=180, Percent_Identity=31.6666666666667, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1788542, Length=171, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=46.6019417475728, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=48.8095238095238, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=48.8095238095238, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=132, Percent_Identity=38.6363636363636, Blast_Score=99, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137554, Length=90, Percent_Identity=37.7777777777778, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20420; Mature: 20420

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKFFLCKFTFYFKNDKGEFYMIYKRFNSPVGVLTVFSSGKGICKIEFENEKFPLEDFE
CEEEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCHHHHC
EGTDFYIEECIKELDLYFQKRLKNFDVKLDLQGTKFQMAVWEETLKIPYGSVVTYGEIAR
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHCCCCCCEEEHHHHHH
RIGKPKAARAVGQALNKNSIPIIIPCHRVIGSDGNLTGFGGGIEIKKFLLKHEGVKIGG
HHCCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEECCCHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MIVKFFLCKFTFYFKNDKGEFYMIYKRFNSPVGVLTVFSSGKGICKIEFENEKFPLEDFE
CEEEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEEECCCCEEEEEECCCCCCHHHHC
EGTDFYIEECIKELDLYFQKRLKNFDVKLDLQGTKFQMAVWEETLKIPYGSVVTYGEIAR
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHCCCCCCEEEHHHHHH
RIGKPKAARAVGQALNKNSIPIIIPCHRVIGSDGNLTGFGGGIEIKKFLLKHEGVKIGG
HHCCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEECCCHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]