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The map label for this gene is ThiF [H]

Identifier: 20808826

GI number: 20808826

Start: 2360519

End: 2361124

Strand: Direct

Name: ThiF [H]

Synonym: TTE2471

Alternate gene names: 20808826

Gene position: 2360519-2361124 (Clockwise)

Preceding gene: 20808825

Following gene: 20808830

Centisome position: 87.77

GC content: 33.99

Gene sequence:

>606_bases
ATGAATCCTTTTGATGTAATGCTTAAAAATTATTTTGATGAAAAGATGCTTGACAAGCTTTCAAGAGTAAAAATTCTTCT
CATTGGCTGTGGTGGACTTGGTTCAAATGTTGCAGTGATGCTTATAAGAAGTGGTATTAAAAACCTCACAATAGTCGATT
TTGATGAGGTAGACATCTTAAATCTCAACCGACAAAATTACTTTTTACACCAGGTAGGAGAAAAAAAGGTATTAGCCCTT
AAAGATACTCTCTTTAAAATCAACCCCCACGCAAACATAGCAGCCAAAAATATAAAAATTGATGAGTCAAATTTAGATGA
GTTAATCCCTCAACATGATATAGTTGTGGAAGCTGTTGACAATGAATTTACCAAAACGCTGATTTTTAAAAAAGCCCAAA
AACATAATAAAAAAGTGGTTTTGGCTTCAGGTGTCGCAGGATTTGGTGATTGCGAAAATATTAAAATAAAACGGGGTAAA
AACTTTTCAATTGTGGGTGATTTTGTTACATCGATTAATGAAAAGAAACCTTTGGCACCCAAAGTCATGGCAGTTGCCGC
AATACAAGCAGATGAAGTATTAAGGATGATAAATGAGCTGGAGTAA

Upstream 100 bases:

>100_bases
CCTAAGGCTGGTAGTATAAAAAATTCTTAAAACCAGCCTTTTTTCTTACACAAAAGTCAACATTTAAAATTACAAACAAA
GTAAGGAGAGTGAAAAATGC

Downstream 100 bases:

>100_bases
AAATGATGATATAATTATTTTTCTTCAAGTATTTTTTTTCGTTCCAGATACTCTTCTTCGCTTATTTCCCCTCTTGCATA
TCTTTCTTTTAAAATTTCTA

Product: thiamine biosynthesis protein ThiF

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNPFDVMLKNYFDEKMLDKLSRVKILLIGCGGLGSNVAVMLIRSGIKNLTIVDFDEVDILNLNRQNYFLHQVGEKKVLAL
KDTLFKINPHANIAAKNIKIDESNLDELIPQHDIVVEAVDNEFTKTLIFKKAQKHNKKVVLASGVAGFGDCENIKIKRGK
NFSIVGDFVTSINEKKPLAPKVMAVAAIQADEVLRMINELE

Sequences:

>Translated_201_residues
MNPFDVMLKNYFDEKMLDKLSRVKILLIGCGGLGSNVAVMLIRSGIKNLTIVDFDEVDILNLNRQNYFLHQVGEKKVLAL
KDTLFKINPHANIAAKNIKIDESNLDELIPQHDIVVEAVDNEFTKTLIFKKAQKHNKKVVLASGVAGFGDCENIKIKRGK
NFSIVGDFVTSINEKKPLAPKVMAVAAIQADEVLRMINELE
>Mature_201_residues
MNPFDVMLKNYFDEKMLDKLSRVKILLIGCGGLGSNVAVMLIRSGIKNLTIVDFDEVDILNLNRQNYFLHQVGEKKVLAL
KDTLFKINPHANIAAKNIKIDESNLDELIPQHDIVVEAVDNEFTKTLIFKKAQKHNKKVVLASGVAGFGDCENIKIKRGK
NFSIVGDFVTSINEKKPLAPKVMAVAAIQADEVLRMINELE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Escherichia coli, GI1787048, Length=199, Percent_Identity=31.6582914572864, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789177, Length=173, Percent_Identity=26.0115606936416, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI87082356, Length=137, Percent_Identity=29.9270072992701, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=189, Percent_Identity=32.2751322751323, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321903, Length=195, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321790, Length=158, Percent_Identity=32.2784810126582, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582879, Length=188, Percent_Identity=26.5957446808511, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24660640, Length=151, Percent_Identity=29.1390728476821, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 22462; Mature: 22462

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFDVMLKNYFDEKMLDKLSRVKILLIGCGGLGSNVAVMLIRSGIKNLTIVDFDEVDIL
CCHHHHHHHHHCCHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCEEEE
NLNRQNYFLHQVGEKKVLALKDTLFKINPHANIAAKNIKIDESNLDELIPQHDIVVEAVD
ECCCCCEEEECCCCCEEEEEEEEEEEECCCCCEEEEEEEECCCCHHHHCCCCCEEEEECC
NEFTKTLIFKKAQKHNKKVVLASGVAGFGDCENIKIKRGKNFSIVGDFVTSINEKKPLAP
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHCCCCCCCCH
KVMAVAAIQADEVLRMINELE
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPFDVMLKNYFDEKMLDKLSRVKILLIGCGGLGSNVAVMLIRSGIKNLTIVDFDEVDIL
CCHHHHHHHHHCCHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCEEEE
NLNRQNYFLHQVGEKKVLALKDTLFKINPHANIAAKNIKIDESNLDELIPQHDIVVEAVD
ECCCCCEEEECCCCCEEEEEEEEEEEECCCCCEEEEEEEECCCCHHHHCCCCCEEEEECC
NEFTKTLIFKKAQKHNKKVVLASGVAGFGDCENIKIKRGKNFSIVGDFVTSINEKKPLAP
CHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHCCCCCCCCH
KVMAVAAIQADEVLRMINELE
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]