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The map label for this gene is NifS2 [H]

Identifier: 20808820

GI number: 20808820

Start: 2354795

End: 2355949

Strand: Reverse

Name: NifS2 [H]

Synonym: TTE2465

Alternate gene names: 20808820

Gene position: 2355949-2354795 (Counterclockwise)

Preceding gene: 20808821

Following gene: 20808819

Centisome position: 87.6

GC content: 35.58

Gene sequence:

>1155_bases
GTGGATAGAATTTATTTAGACAATGCTGCGACTACACCAGTTGATAAAAGGGTATTAGAGGCGATGTTACCATATTATAG
TGATGTTTTTGGTAATCCATCTTCCCTGTATTCATATGGACAAGAAGCGAAAAAAGCTATTGAAGAAGCGAGGGAAAAAG
TGGCAAAAGCACTAGGGGCAGATTCTGACGAAATATATTTTACCAGTGGTGGGTCTGAGTCTGATAATTGGGCATTAAAA
GGTGTAGCATATGCGTTAAAAGATAAGGGAAATCACATTATTACAACGGAAATTGAACATCATGCAGTACTTAATACTTG
TCGATATCTTGAAAAAGAAGGATTCAAAGTAACATATCTTCCAGTTGACGAATATGGACTTGTAAAACCTGAAGATTTAA
AAAAGGCAATTACAGATAAAACAATCCTTGTTTCTATAATGTTTGCCAATAATGAGATAGGTACGATAGAGCCAATTGAG
GAGCTTGTCAAAATAGCTCATGAGAAAAATGTATATTTTCATACTGATGCTGTGCAAGCAGTTGGTAATGTACCAATAGA
TGTAAAAAAATTAGATGTTGATTTGTTATCTCTATCTGCCCATAAAATATATGGACCTAAAGGCGTGGGAGCATTATATA
TAAAGAAGGGTGTAAAGATTCATTCGCTTATACAAGGTGGAACACAGGAAAGGAATAGAAGAGCGGGCACAGAAAATGTT
CCAGGAATAGTAGGACTGGGAGAAGCGATGGAGCTTATCACGAAAAATTTGGATTCTCATATAAATAAACTTACATTTTT
AAGAGATAAACTTATAAATGGAATATTAGAAAAAATACCATATGCGAGGTTAAACGGGCATCCAACAAAAAGGCTTCCAG
GCAATGTTAATGTATCATTTGAATTTATAGATGGCGAATCCCTTATTTTAAACTTAGATATGGCAGGAATATGTGCTTCA
AGTGGTTCAGCATGCACTTCTGGTTCATTGGAGCCCTCTCATGTGCTTCTGGCAATTGGGCTTCCTCGCGAATTAGCTCG
AGGGTCGTTAAGACTTACAATTGGTAAAGAAAACACGGAAGAAGATATAGATAAAGTTTTAGAAGTACTTCCACAAATTA
TTAAAAGATTAAGGTCAATATCACAGATTGTATGA

Upstream 100 bases:

>100_bases
GTGGTATTTTATATTTAAGTATGCACAAATTTTTTGAATAAAAAATGTAAAATATGAACAGATACTAAGAAGTAGAAAAT
AATAGAGAGAGGCGATTTTT

Downstream 100 bases:

>100_bases
GAGAATCTACAAGGAGGTTAGAGAATTTATCTATGCAGAAAAACAACAGAGTAGTTGTGGGAATGAGCGGAGGTGTCGAT
AGCTCTGTTGCGGCATATCT

Product: cysteine sulfinate desulfinase/cysteine desulfurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSLYSYGQEAKKAIEEAREKVAKALGADSDEIYFTSGGSESDNWALK
GVAYALKDKGNHIITTEIEHHAVLNTCRYLEKEGFKVTYLPVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIE
ELVKIAHEKNVYFHTDAVQAVGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
PGIVGLGEAMELITKNLDSHINKLTFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSFEFIDGESLILNLDMAGICAS
SGSACTSGSLEPSHVLLAIGLPRELARGSLRLTIGKENTEEDIDKVLEVLPQIIKRLRSISQIV

Sequences:

>Translated_384_residues
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSLYSYGQEAKKAIEEAREKVAKALGADSDEIYFTSGGSESDNWALK
GVAYALKDKGNHIITTEIEHHAVLNTCRYLEKEGFKVTYLPVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIE
ELVKIAHEKNVYFHTDAVQAVGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
PGIVGLGEAMELITKNLDSHINKLTFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSFEFIDGESLILNLDMAGICAS
SGSACTSGSLEPSHVLLAIGLPRELARGSLRLTIGKENTEEDIDKVLEVLPQIIKRLRSISQIV
>Mature_384_residues
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSLYSYGQEAKKAIEEAREKVAKALGADSDEIYFTSGGSESDNWALK
GVAYALKDKGNHIITTEIEHHAVLNTCRYLEKEGFKVTYLPVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIE
ELVKIAHEKNVYFHTDAVQAVGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
PGIVGLGEAMELITKNLDSHINKLTFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSFEFIDGESLILNLDMAGICAS
SGSACTSGSLEPSHVLLAIGLPRELARGSLRLTIGKENTEEDIDKVLEVLPQIIKRLRSISQIV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=48.3028720626632, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=34.9514563106796, Blast_Score=229, Evalue=5e-60,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=47.5195822454308, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=28.8607594936709, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=27.020202020202, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=50.1291989664083, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=33.6363636363636, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319831, Length=383, Percent_Identity=48.8250652741514, Blast_Score=355, Evalue=5e-99,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=48.3028720626632, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42179; Mature: 42179

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSLYSYGQEAKKAIEEAREKVAKALGA
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSDEIYFTSGGSESDNWALKGVAYALKDKGNHIITTEIEHHAVLNTCRYLEKEGFKVTYL
CCCEEEEECCCCCCCCEEHHHHHEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEE
PVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIEELVKIAHEKNVYFHTDAVQA
ECCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
VGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
HCCCCCEEEEECHHHEEHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHCCCCCCC
PGIVGLGEAMELITKNLDSHINKLTFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
EFIDGESLILNLDMAGICASSGSACTSGSLEPSHVLLAIGLPRELARGSLRLTIGKENTE
EEECCCEEEEEECHHHEECCCCCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEECCCCCH
EDIDKVLEVLPQIIKRLRSISQIV
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRIYLDNAATTPVDKRVLEAMLPYYSDVFGNPSSLYSYGQEAKKAIEEAREKVAKALGA
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSDEIYFTSGGSESDNWALKGVAYALKDKGNHIITTEIEHHAVLNTCRYLEKEGFKVTYL
CCCEEEEECCCCCCCCEEHHHHHEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEE
PVDEYGLVKPEDLKKAITDKTILVSIMFANNEIGTIEPIEELVKIAHEKNVYFHTDAVQA
ECCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEECHHHHH
VGNVPIDVKKLDVDLLSLSAHKIYGPKGVGALYIKKGVKIHSLIQGGTQERNRRAGTENV
HCCCCCEEEEECHHHEEHHHHHCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHCCCCCCC
PGIVGLGEAMELITKNLDSHINKLTFLRDKLINGILEKIPYARLNGHPTKRLPGNVNVSF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
EFIDGESLILNLDMAGICASSGSACTSGSLEPSHVLLAIGLPRELARGSLRLTIGKENTE
EEECCCEEEEEECHHHEECCCCCCCCCCCCCCCEEEEEECCCHHHCCCCEEEEECCCCCH
EDIDKVLEVLPQIIKRLRSISQIV
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]