Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 20808683

GI number: 20808683

Start: 2197902

End: 2199359

Strand: Reverse

Name: gltX

Synonym: TTE2317

Alternate gene names: 20808683

Gene position: 2199359-2197902 (Counterclockwise)

Preceding gene: 20808684

Following gene: 20808682

Centisome position: 81.78

GC content: 38.89

Gene sequence:

>1458_bases
ATGAAAGAATTTAGGGTGAGATTCGCACCAAGCCCTACAGGACCGATACACATAGGTAACATGAGAACAGCTCTTTTTAA
TTATCTTTTTTCGAGGAGCGAAGGAGCTACTTTTGTATTAAGAATTGAAGATACTGATTTAGAGAGGTCTTCAAAGGGGT
TTGAAGAATTAATATTCAAAGAGTTAAAGTGGCTGGGCATAGAGTGGGATGAGGGGCCAGATAAACCAGGTCCATATGGC
CCTTACAGGCAGAGTGAAAGGCTTGAGATCTACCACAGATTTGCTCAAAAGCTTATTGAAGAGAAAAAAGCTTACAGGTG
CTACTGCACGCCAGAAGAGCTGGAAGAAGACAGGAAGAAAGCGATAGAGAGGGGAGAAATCCCCCGTTATTCTGGCAGAT
GTAGGTACCTCACAAAAGAGCAGGAAGAAACTTTTATAAGGGAAGGAAGAAAGCCTGTCATAAGGTTTATGGTGCCAGAC
GATGAAGTCATAGAATTTGAAGACATGATAAAGGGAAAGATTACAATAAAAAGTGACACATTAGGCGGAGACATGGTGAT
TATAAAGTCTGACGGCATGCCAACTTATAATTTTGCTGTGGTGATTGACGATGCTTTAATGAAAATCACCCATGTGATAA
GAGGGGAAGACCACATATACAATACTCCAAAGCAGATTTTGATATATAAAGCTTTAGGATTTGAGATTCCTAAGTTTGCT
CATGTTCCTTTGATATTGGGGCCTGATAGGACAAAGCTTTCCAAAAGGCACGGCCATACCTACATAGGTCAGTACAGGGA
ATTAGGGTATTTGCCAGAAGCCATGTTTAATTTCTTATCACTTTTGAGCTGGTATCCTGAGGATAATGTGGAATTGATGT
CAAAAGAAGAGATAATAAGAAAGTTCAATTTTAAGAGGATTCACAAATCAAATCCAGTATTTGACATTGAAAAGTTAAAC
TGGATGAACCAGCAGTACATACAAAAAAGCCCTGTAGAAAGGATTGTGGATTTGGCAATTCCTCATCTTAAGAAAGCAGG
CTACATTGACGAAATAGACGAGTTGAGGTATAATTGGCTAAAGGATGTGATAAGCCTCTATAAAGATGGACTATCTTATG
TAGCTCAAATTGTAGACATGGCAAAGACTTTCTTTGTTGAAGAAGTAGATTACACTGGAGAAATGATAGAGTTTTTAAAA
AGTCCCAATTCTATAAAAGTATTGGAGGCTTTTAAAGGTTATTTGAAGGATAAGAGCGAAATTACTGAGGATGATGTTAG
GGAGTGGATGAAGAAAGCTCAGAAAGAGCTTGGGGTAAAAGGAAAAGAGTTTTTCATGCCTATAAGGATAGCAGTAACGG
GAGAAGAGCACGGCCCTGAGCTTGTAAAAGTCCTCGCACTTTTGGGGAAGAATAGGGTAGTAAAAAGGCTTGACAGAGTT
TTAAACTTAATAGAGTAA

Upstream 100 bases:

>100_bases
AAATATGTGGATTTAAAATAGGATTGTCACTTCATCTATTAAATGAGCATAAAATCTGTTATAATTAAATCAAACAACAA
ACGAGGATGGAGTGACAAAA

Downstream 100 bases:

>100_bases
AAGGCGATGACAAGAAGAGTATTATGGCACAAGCCTTCCAGAGAGGAGCCATCGTGGGCTGGAAGTGGCTCTAAGGATGT
GCCATAAGAAGTGCGTCTTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKEFRVRFAPSPTGPIHIGNMRTALFNYLFSRSEGATFVLRIEDTDLERSSKGFEELIFKELKWLGIEWDEGPDKPGPYG
PYRQSERLEIYHRFAQKLIEEKKAYRCYCTPEELEEDRKKAIERGEIPRYSGRCRYLTKEQEETFIREGRKPVIRFMVPD
DEVIEFEDMIKGKITIKSDTLGGDMVIIKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
HVPLILGPDRTKLSKRHGHTYIGQYRELGYLPEAMFNFLSLLSWYPEDNVELMSKEEIIRKFNFKRIHKSNPVFDIEKLN
WMNQQYIQKSPVERIVDLAIPHLKKAGYIDEIDELRYNWLKDVISLYKDGLSYVAQIVDMAKTFFVEEVDYTGEMIEFLK
SPNSIKVLEAFKGYLKDKSEITEDDVREWMKKAQKELGVKGKEFFMPIRIAVTGEEHGPELVKVLALLGKNRVVKRLDRV
LNLIE

Sequences:

>Translated_485_residues
MKEFRVRFAPSPTGPIHIGNMRTALFNYLFSRSEGATFVLRIEDTDLERSSKGFEELIFKELKWLGIEWDEGPDKPGPYG
PYRQSERLEIYHRFAQKLIEEKKAYRCYCTPEELEEDRKKAIERGEIPRYSGRCRYLTKEQEETFIREGRKPVIRFMVPD
DEVIEFEDMIKGKITIKSDTLGGDMVIIKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
HVPLILGPDRTKLSKRHGHTYIGQYRELGYLPEAMFNFLSLLSWYPEDNVELMSKEEIIRKFNFKRIHKSNPVFDIEKLN
WMNQQYIQKSPVERIVDLAIPHLKKAGYIDEIDELRYNWLKDVISLYKDGLSYVAQIVDMAKTFFVEEVDYTGEMIEFLK
SPNSIKVLEAFKGYLKDKSEITEDDVREWMKKAQKELGVKGKEFFMPIRIAVTGEEHGPELVKVLALLGKNRVVKRLDRV
LNLIE
>Mature_485_residues
MKEFRVRFAPSPTGPIHIGNMRTALFNYLFSRSEGATFVLRIEDTDLERSSKGFEELIFKELKWLGIEWDEGPDKPGPYG
PYRQSERLEIYHRFAQKLIEEKKAYRCYCTPEELEEDRKKAIERGEIPRYSGRCRYLTKEQEETFIREGRKPVIRFMVPD
DEVIEFEDMIKGKITIKSDTLGGDMVIIKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
HVPLILGPDRTKLSKRHGHTYIGQYRELGYLPEAMFNFLSLLSWYPEDNVELMSKEEIIRKFNFKRIHKSNPVFDIEKLN
WMNQQYIQKSPVERIVDLAIPHLKKAGYIDEIDELRYNWLKDVISLYKDGLSYVAQIVDMAKTFFVEEVDYTGEMIEFLK
SPNSIKVLEAFKGYLKDKSEITEDDVREWMKKAQKELGVKGKEFFMPIRIAVTGEEHGPELVKVLALLGKNRVVKRLDRV
LNLIE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=33.0645161290323, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1788743, Length=482, Percent_Identity=38.1742738589212, Blast_Score=348, Evalue=7e-97,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=34, Blast_Score=138, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=32.9918032786885, Blast_Score=248, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6324540, Length=502, Percent_Identity=33.0677290836653, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324742, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=334, Percent_Identity=25.748502994012, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=35.010060362173, Blast_Score=288, Evalue=9e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_THETN (Q8R7T1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623854.1
- ProteinModelPortal:   Q8R7T1
- SMR:   Q8R7T1
- GeneID:   996607
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2317
- NMPDR:   fig|273068.3.peg.2495
- HOGENOM:   HBG628189
- OMA:   WINNQYV
- ProtClustDB:   PRK01406
- BioCyc:   TTEN273068:TTE2317-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56825; Mature: 56825

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFRVRFAPSPTGPIHIGNMRTALFNYLFSRSEGATFVLRIEDTDLERSSKGFEELIFK
CCCCEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHCCCHHHHHHH
ELKWLGIEWDEGPDKPGPYGPYRQSERLEIYHRFAQKLIEEKKAYRCYCTPEELEEDRKK
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHH
AIERGEIPRYSGRCRYLTKEQEETFIREGRKPVIRFMVPDDEVIEFEDMIKGKITIKSDT
HHHHCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHCCEEEEEECC
LGGDMVIIKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCHHCCCCHHEEEEHHHCCCCCCCC
HVPLILGPDRTKLSKRHGHTYIGQYRELGYLPEAMFNFLSLLSWYPEDNVELMSKEEIIR
CCCEEECCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KFNFKRIHKSNPVFDIEKLNWMNQQYIQKSPVERIVDLAIPHLKKAGYIDEIDELRYNWL
HHCHHHHHCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KDVISLYKDGLSYVAQIVDMAKTFFVEEVDYTGEMIEFLKSPNSIKVLEAFKGYLKDKSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ITEDDVREWMKKAQKELGVKGKEFFMPIRIAVTGEEHGPELVKVLALLGKNRVVKRLDRV
CCHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHH
LNLIE
HHHCC
>Mature Secondary Structure
MKEFRVRFAPSPTGPIHIGNMRTALFNYLFSRSEGATFVLRIEDTDLERSSKGFEELIFK
CCCCEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHCCCHHHHHHH
ELKWLGIEWDEGPDKPGPYGPYRQSERLEIYHRFAQKLIEEKKAYRCYCTPEELEEDRKK
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHH
AIERGEIPRYSGRCRYLTKEQEETFIREGRKPVIRFMVPDDEVIEFEDMIKGKITIKSDT
HHHHCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHCCEEEEEECC
LGGDMVIIKSDGMPTYNFAVVIDDALMKITHVIRGEDHIYNTPKQILIYKALGFEIPKFA
CCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCHHCCCCHHEEEEHHHCCCCCCCC
HVPLILGPDRTKLSKRHGHTYIGQYRELGYLPEAMFNFLSLLSWYPEDNVELMSKEEIIR
CCCEEECCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KFNFKRIHKSNPVFDIEKLNWMNQQYIQKSPVERIVDLAIPHLKKAGYIDEIDELRYNWL
HHCHHHHHCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KDVISLYKDGLSYVAQIVDMAKTFFVEEVDYTGEMIEFLKSPNSIKVLEAFKGYLKDKSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
ITEDDVREWMKKAQKELGVKGKEFFMPIRIAVTGEEHGPELVKVLALLGKNRVVKRLDRV
CCHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHH
LNLIE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336