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The map label for this gene is TufB2 [H]

Identifier: 20808676

GI number: 20808676

Start: 2191776

End: 2192978

Strand: Reverse

Name: TufB2 [H]

Synonym: TTE2310

Alternate gene names: 20808676

Gene position: 2192978-2191776 (Counterclockwise)

Preceding gene: 20808677

Following gene: 20808675

Centisome position: 81.54

GC content: 49.04

Gene sequence:

>1203_bases
ATGGCAAAGCAGAAATTTGAGAGGACGAAGCCGCACGTAAACGTAGGGACGATAGGGCACGTAGACCATGGTAAGACGAC
ATTAACAGCAGCGATAACATTAATACTTTCGAAAGCGGGATTAGCACAGGCGAAGGGATATGACGAGATAGACAAGGCGC
CAGAGGAGAAGGCAAGGGGTATAACAATAAACACGACGCACGTAGAGTATGAGACAGCGAAGAGGCACTATGCGCACGTA
GACTGTCCGGGTCACGCGGACTATGTAAAGAACATGATAACAGGGGCAGCGCAGATGGACGGAGCGATACTGGTAGTATC
AGCAGCAGACGGTCCGATGCCGCAGACGAGGGAGCACATATTATTGGCAAGGCAGGTAGGAGTGCCATATATAGTAGTAT
TTTTGAACAAAGCGGACATGGTAGACGATCCGGAGTTAATAGAGCTAGTAGAGATGGAGGTAAGGGACTTACTGAACCAG
TACGAATTTCCAGGGGATGAGACACCGATAGTGGTAGGTTCAGCATTGAAGGCATTAGAGTGCGGATGTGGCAAGAGGGA
GTGCCAGTGGTGCGGCAAGATATGGGAGTTAATGGATGTAGTAGACGAATATATACCGACACCGGAGAGGGATATAGACA
AGCCGTTCTTGATGCCGGTAGAGGACGTATTTTCGATAACGGGAAGAGGTACAGTTGCGACAGGTAGAGTAGAGAGAGGG
AAAGTGAAAGTAGGGGACGAAGTAGAGATAATAGGATTGACGACGGAGTCAAGGAAGACAGTAGTGACAGGAGTAGAGAT
GTTCAGGAAGACATTAGATGAGGCACAGGCAGGAGACAACATAGGGGTATTGTTAAGAGGGATACAGAAGGATGAAGTAG
AGAGAGGGCAGGTACTGGCGAAACCAGGGACGATAAAGCCGCACACGAAATTTGAGGCACAGGTGTACGTATTGACGAAG
GAAGAGGGAGGAAGGCACACACCATTTTTCAATGGATACAGGCCGCAGTTTTACTTTAGGACGACGGACGTGACAGGGAC
GATACAGCTGCCAGAGGGAGTAGAGATGGTAATGCCAGGGGACCACGTGACATTGAGAGTAGAGCTTATAACGCCGATAG
CGATGGAAGAAGGGCTTAAGTTTGCTATAAGAGAAGGCGGAAGGACAGTTGGCGCTGGTGTCGTGTCTGCTATAATTGAG
TAA

Upstream 100 bases:

>100_bases
AATTTACGCTTTAGTTGTTTTTTTTTATCAAAGAAGTGTTCTTCACTTTATTAATAAGAAACACTTGGATAAGTTTATTA
GAAGTAAGGAGGAAGAGAGG

Downstream 100 bases:

>100_bases
AACAGGAAGCCGGGTGCAAACCTGGCTTCTTTTGGGTTAATTTATTTTTGTTTAAGGATTGACAACAAGTTTTATTTATG
ATAAATTTGTCTAAGTAGTC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITLILSKAGLAQAKGYDEIDKAPEEKARGITINTTHVEYETAKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRDLLNQ
YEFPGDETPIVVGSALKALECGCGKRECQWCGKIWELMDVVDEYIPTPERDIDKPFLMPVEDVFSITGRGTVATGRVERG
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTLDEAQAGDNIGVLLRGIQKDEVERGQVLAKPGTIKPHTKFEAQVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGTIQLPEGVEMVMPGDHVTLRVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE

Sequences:

>Translated_400_residues
MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITLILSKAGLAQAKGYDEIDKAPEEKARGITINTTHVEYETAKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRDLLNQ
YEFPGDETPIVVGSALKALECGCGKRECQWCGKIWELMDVVDEYIPTPERDIDKPFLMPVEDVFSITGRGTVATGRVERG
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTLDEAQAGDNIGVLLRGIQKDEVERGQVLAKPGTIKPHTKFEAQVYVLTK
EEGGRHTPFFNGYRPQFYFRTTDVTGTIQLPEGVEMVMPGDHVTLRVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
>Mature_399_residues
AKQKFERTKPHVNVGTIGHVDHGKTTLTAAITLILSKAGLAQAKGYDEIDKAPEEKARGITINTTHVEYETAKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRDLLNQY
EFPGDETPIVVGSALKALECGCGKRECQWCGKIWELMDVVDEYIPTPERDIDKPFLMPVEDVFSITGRGTVATGRVERGK
VKVGDEVEIIGLTTESRKTVVTGVEMFRKTLDEAQAGDNIGVLLRGIQKDEVERGQVLAKPGTIKPHTKFEAQVYVLTKE
EGGRHTPFFNGYRPQFYFRTTDVTGTIQLPEGVEMVMPGDHVTLRVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=58.4810126582278, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=29.1196388261851, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=29.2906178489703, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=29.5964125560538, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=28.4116331096197, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI54607086, Length=349, Percent_Identity=29.512893982808, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018522, Length=348, Percent_Identity=27.8735632183908, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI194018520, Length=348, Percent_Identity=27.8735632183908, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI46094014, Length=348, Percent_Identity=27.5862068965517, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194097354, Length=348, Percent_Identity=27.8735632183908, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4503507, Length=275, Percent_Identity=28.7272727272727, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI157426893, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1790412, Length=399, Percent_Identity=75.9398496240601, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1789737, Length=399, Percent_Identity=75.9398496240601, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=343, Percent_Identity=30.3206997084548, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI48994988, Length=284, Percent_Identity=31.3380281690141, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=260, Percent_Identity=30.3846153846154, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790835, Length=176, Percent_Identity=29.5454545454545, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=52.4875621890547, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=391, Percent_Identity=44.2455242966752, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=30.242825607064, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=30.242825607064, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=29.5348837209302, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=27.1493212669683, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=27.1070615034169, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=457, Percent_Identity=27.1334792122538, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=30.5921052631579, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=39.5973154362416, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=27.3049645390071, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=240, Percent_Identity=27.9166666666667, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=65.9147869674186, Blast_Score=541, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=29.8876404494382, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=29.8876404494382, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=346, Percent_Identity=27.7456647398844, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322937, Length=354, Percent_Identity=24.0112994350282, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=295, Percent_Identity=27.1186440677966, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=29.2181069958848, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=141, Percent_Identity=32.6241134751773, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=399, Percent_Identity=57.3934837092732, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=399, Percent_Identity=57.3934837092732, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=391, Percent_Identity=50.1278772378517, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.2631578947368, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.2631578947368, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.2631578947368, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.2631578947368, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=29.8206278026906, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=29.8206278026906, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=32.6860841423948, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI45550900, Length=452, Percent_Identity=28.7610619469027, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=31.3782991202346, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647048, Length=424, Percent_Identity=26.4150943396226, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647046, Length=424, Percent_Identity=26.4150943396226, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=37.3493975903614, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44009; Mature: 43878

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKFERTKPHVNVGTIGHVDHGKTTLTAAITLILSKAGLAQAKGYDEIDKAPEEKARG
CCCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHCCHHHHHCC
ITINTTHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEHHHHCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRDLLNQYEFPGDETPIVVGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHH
CGCGKRECQWCGKIWELMDVVDEYIPTPERDIDKPFLMPVEDVFSITGRGTVATGRVERG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCEECC
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTLDEAQAGDNIGVLLRGIQKDEVERGQVLA
EEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCEEEE
KPGTIKPHTKFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGTIQLPEGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECC
DHVTLRVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
CCEEEEEEEECCHHHHCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKQKFERTKPHVNVGTIGHVDHGKTTLTAAITLILSKAGLAQAKGYDEIDKAPEEKARG
CCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHCCHHHHHCC
ITINTTHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEHHHHCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDPELIELVEMEVRDLLNQYEFPGDETPIVVGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHH
CGCGKRECQWCGKIWELMDVVDEYIPTPERDIDKPFLMPVEDVFSITGRGTVATGRVERG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCEECC
KVKVGDEVEIIGLTTESRKTVVTGVEMFRKTLDEAQAGDNIGVLLRGIQKDEVERGQVLA
EEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCEEEE
KPGTIKPHTKFEAQVYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGTIQLPEGVEMVMPG
CCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECC
DHVTLRVELITPIAMEEGLKFAIREGGRTVGAGVVSAIIE
CCEEEEEEEECCHHHHCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]