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The map label for this gene is GdhA4 [H]

Identifier: 20808583

GI number: 20808583

Start: 2112645

End: 2113712

Strand: Reverse

Name: GdhA4 [H]

Synonym: TTE2203

Alternate gene names: 20808583

Gene position: 2113712-2112645 (Counterclockwise)

Preceding gene: 20808584

Following gene: 20808582

Centisome position: 78.59

GC content: 42.51

Gene sequence:

>1068_bases
ATGAAAATTTTTGAGCGGATGGAAGTCAAGGATTATGAAAACGTAATTTTTTGTCATGATAAAGCGTCGGGTTTAAAAGC
AATAATTGTAATTCACGATACAACTTTAGGCCCAGCTCTTGGCGGAACTAGGATGTGGATGTACAATACAGAAGAAGAGG
CACTGGAAGATGCTTTAAGGCTTGCAAGGGGAATGACATATAAAAACGCTGCCGCCGGCTTAAACTACGGAGGAGGGAAG
GCAGTAATAATAGGCGATCCTAGAAAAGATAAATCAGAAGCCCTCTTTAGAAGTTTTGGAAGGTATATAGAAGCTCTGAA
AGGACGCTTTATAACAGGTGAGGACGTGGGGATCACTGTCCAGGACGTTGAATACATGAGGTATGAGACAAAGCACGTGG
TTGGCCTTGCTGAGACCAGCGGAGACCCGTCGCCTGTCACTGCTTACGGAGTGTACAGAGGTATGCAGGCAGCTGCTGAA
GAAGTGTTTGGGACTTCCGACTTAAAAGGCAAAAAAGTAGCCATACAGGGTGTTGGAAAAGTTGGCTATACTCTTGCTAA
ATATCTTAATGAAGCTGGAGCGAAAATAATTGTCACTGATATATTTGAAGATAATGTAAAGAGGGTTGTGGAAGACTTCG
GAGCAGAATATGTGAAGCCAGAGGAAATATACGGAGTAGACTGCGATATTTTTGCTCCTTGCGCATTGGGCGGAGTCATA
AACGATGAAACAATACCGCAGCTCAAGTGCAAAATCATAGCAGGTTCTGCCAACAATCAATTGAAAGAAGAAAGGCATGG
GGACATACTGCAGGAAAAAGGTATTCTTTACGTGCCTGACTATATAATAAACGCGGGCGGAGTTATAAACGTGGCTGATG
AGTTAGAACCCTCAGGCTACAACAGGGAAAGGGCTTTGAGGAAGGCTTCCATGATATACGACACGATAAAGAGCGTAATT
AAAAAGTCAAAAGAAGAAAACATACCCACCTACAAAGCTGCCGATAGAGTAGCAGAAGATAGGATTAAAGCGATTGCTGC
AATAAAGACCAATTACATTTTGAAATAA

Upstream 100 bases:

>100_bases
GTACTGACTTCAAGAGCTGATTCCAGCGATGCAAAATTTAATTCCATAATGCTGGCTTCTCTCGCTTCTAATGTTTAAAA
AATTTACTGGGGGGTTATTT

Downstream 100 bases:

>100_bases
TTTAAATTTAAAATTAAAAATAAAGGCAAGGAGGAGATTTTATGGAGATTTTCAAAATAATGGAGAAATATGATTACGAG
AATGTAGTGCTCTGCTATGA

Product: glutamate dehydrogenase/leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIFERMEVKDYENVIFCHDKASGLKAIIVIHDTTLGPALGGTRMWMYNTEEEALEDALRLARGMTYKNAAAGLNYGGGK
AVIIGDPRKDKSEALFRSFGRYIEALKGRFITGEDVGITVQDVEYMRYETKHVVGLAETSGDPSPVTAYGVYRGMQAAAE
EVFGTSDLKGKKVAIQGVGKVGYTLAKYLNEAGAKIIVTDIFEDNVKRVVEDFGAEYVKPEEIYGVDCDIFAPCALGGVI
NDETIPQLKCKIIAGSANNQLKEERHGDILQEKGILYVPDYIINAGGVINVADELEPSGYNRERALRKASMIYDTIKSVI
KKSKEENIPTYKAADRVAEDRIKAIAAIKTNYILK

Sequences:

>Translated_355_residues
MKIFERMEVKDYENVIFCHDKASGLKAIIVIHDTTLGPALGGTRMWMYNTEEEALEDALRLARGMTYKNAAAGLNYGGGK
AVIIGDPRKDKSEALFRSFGRYIEALKGRFITGEDVGITVQDVEYMRYETKHVVGLAETSGDPSPVTAYGVYRGMQAAAE
EVFGTSDLKGKKVAIQGVGKVGYTLAKYLNEAGAKIIVTDIFEDNVKRVVEDFGAEYVKPEEIYGVDCDIFAPCALGGVI
NDETIPQLKCKIIAGSANNQLKEERHGDILQEKGILYVPDYIINAGGVINVADELEPSGYNRERALRKASMIYDTIKSVI
KKSKEENIPTYKAADRVAEDRIKAIAAIKTNYILK
>Mature_355_residues
MKIFERMEVKDYENVIFCHDKASGLKAIIVIHDTTLGPALGGTRMWMYNTEEEALEDALRLARGMTYKNAAAGLNYGGGK
AVIIGDPRKDKSEALFRSFGRYIEALKGRFITGEDVGITVQDVEYMRYETKHVVGLAETSGDPSPVTAYGVYRGMQAAAE
EVFGTSDLKGKKVAIQGVGKVGYTLAKYLNEAGAKIIVTDIFEDNVKRVVEDFGAEYVKPEEIYGVDCDIFAPCALGGVI
NDETIPQLKCKIIAGSANNQLKEERHGDILQEKGILYVPDYIINAGGVINVADELEPSGYNRERALRKASMIYDTIKSVI
KKSKEENIPTYKAADRVAEDRIKAIAAIKTNYILK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=302, Percent_Identity=27.1523178807947, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI4885281, Length=302, Percent_Identity=26.158940397351, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24649283, Length=296, Percent_Identity=28.7162162162162, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45553475, Length=304, Percent_Identity=27.3026315789474, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45549226, Length=304, Percent_Identity=27.3026315789474, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24649460, Length=271, Percent_Identity=27.6752767527675, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 39103; Mature: 39103

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFERMEVKDYENVIFCHDKASGLKAIIVIHDTTLGPALGGTRMWMYNTEEEALEDALR
CCHHHHCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNYGGGKAVIIGDPRKDKSEALFRSFGRYIEALKGRFITGEDVGITV
HHHCCCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCEEE
QDVEYMRYETKHVVGLAETSGDPSPVTAYGVYRGMQAAAEEVFGTSDLKGKKVAIQGVGK
HHHHHHHHHHHHEEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCH
VGYTLAKYLNEAGAKIIVTDIFEDNVKRVVEDFGAEYVKPEEIYGVDCDIFAPCALGGVI
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHCCCC
NDETIPQLKCKIIAGSANNQLKEERHGDILQEKGILYVPDYIINAGGVINVADELEPSGY
CCCCCCCEEEEEEECCCCCHHHHHHCCCHHHHCCCEEECHHHCCCCCEEEEHHHCCCCCC
NRERALRKASMIYDTIKSVIKKSKEENIPTYKAADRVAEDRIKAIAAIKTNYILK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIFERMEVKDYENVIFCHDKASGLKAIIVIHDTTLGPALGGTRMWMYNTEEEALEDALR
CCHHHHCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHH
LARGMTYKNAAAGLNYGGGKAVIIGDPRKDKSEALFRSFGRYIEALKGRFITGEDVGITV
HHHCCCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCEEE
QDVEYMRYETKHVVGLAETSGDPSPVTAYGVYRGMQAAAEEVFGTSDLKGKKVAIQGVGK
HHHHHHHHHHHHEEEEECCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCH
VGYTLAKYLNEAGAKIIVTDIFEDNVKRVVEDFGAEYVKPEEIYGVDCDIFAPCALGGVI
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHCCCC
NDETIPQLKCKIIAGSANNQLKEERHGDILQEKGILYVPDYIINAGGVINVADELEPSGY
CCCCCCCEEEEEEECCCCCHHHHHHCCCHHHHCCCEEECHHHCCCCCEEEEHHHCCCCCC
NRERALRKASMIYDTIKSVIKKSKEENIPTYKAADRVAEDRIKAIAAIKTNYILK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]