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The map label for this gene is buk1

Identifier: 20808581

GI number: 20808581

Start: 2110371

End: 2111444

Strand: Reverse

Name: buk1

Synonym: TTE2201

Alternate gene names: 20808581

Gene position: 2111444-2110371 (Counterclockwise)

Preceding gene: 20808582

Following gene: 20808580

Centisome position: 78.51

GC content: 42.92

Gene sequence:

>1074_bases
ATGGGGTTGGCTTTAATTTTAGTAATAAACCCTGGTTCTACTTCAACGAAAGTGGCAGTTTTTAGGGACAAAGAGCCTGT
CTTTACAGAGACTTTGAGGCATTCGACAGAGGAATTGAGCAAATACAAGAGAATAATTGACCAGTTTGAATTCAGGACAC
AAGCTATCCTTGATATGTTGAAAGAAAAAGGTATTTCTCTATCTCAAATTGATGCAATTGTAGGAAGAGGAGGACTTTTA
AAGCCCATAGAGAGTGGGACCTACATTGTGAATGAAAAAATGCTGGAAGATTTGAAAAAGGCTGAAAGAGGTGAACACGC
TTCTAATTTGGGAGCTATTATCGCCTACACTTTGGCAAAAGAGCACAACATCCCTGCCTATATAGTGGACCCTGTAGTGG
TGGATGAGCTTGAAGATGTAGCGAGAATTACTGGTTTGCCGGAGATTGAGAAACAGAGCATTTTCCACGCCTTAAACCAA
AAGGCAATTGCTCGCCGCTTGGCATCTGATTTGGGCAAAAGGTACGATGAGGTAAACCTTATAATAGCGCACCTTGGGGG
TGGTATTTCAGTAGGTGCCCATAGAAAAGGAAGGGTAATAGACGTGAATGACGCTCTAAACGGAGAAGGGCCATTTTCCC
CCGAAAGGGCAGGGGGGTTACCTGTTCTAGATCTAGTCAAACTTTGCTACAGTGGGAAGTACACTTTTGAGGAGATGAAG
AAAAAGCTTATAGGAAAAGGCGGAATAGTGGCACATTTGGGTACAAATGATGTGAGAGAAGTTTACAAAATGATTGAGAA
TGGGGATAAAAATGCTGAACTCATTTTGGACGCCATGGCGTATCAGACGGCAAAAGAAATAGGGTCAATGGCTGTCGTTT
TAAAAGGGAAAGTGGATGCCATAGGGATTACTGGCGGAATTGCTCATAATGAGGATTTTGTGAGGAGGATAAGGGAAAGG
GTGGAGTTTATAGCGCCTGTGTATGTATACCCTGGAGAGGACGAAATGCTGGCTTTGGCAGAGGGAGCATACAGGGTTTT
GACAGGCGAAGAGAATCCAAAAACATACAGTTAA

Upstream 100 bases:

>100_bases
GTAAGATAATGTTAATCTGAGGAAAGGCAAGAATTCCTTGCCTTTCCTCAGATTTGGATAAAGAAGCCATGTGTACTATA
ATAGTATTAGAGGCGGAGGG

Downstream 100 bases:

>100_bases
AGGATGAGGTCAGATGGATTTTAAAAGGCTGACGATTGTAGTAGGGCACTACGGCAGTGGTAAGACTGAGATTTCGATAA
ACTATGCTGTTTACATCAGG

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MGLALILVINPGSTSTKVAVFRDKEPVFTETLRHSTEELSKYKRIIDQFEFRTQAILDMLKEKGISLSQIDAIVGRGGLL
KPIESGTYIVNEKMLEDLKKAERGEHASNLGAIIAYTLAKEHNIPAYIVDPVVVDELEDVARITGLPEIEKQSIFHALNQ
KAIARRLASDLGKRYDEVNLIIAHLGGGISVGAHRKGRVIDVNDALNGEGPFSPERAGGLPVLDLVKLCYSGKYTFEEMK
KKLIGKGGIVAHLGTNDVREVYKMIENGDKNAELILDAMAYQTAKEIGSMAVVLKGKVDAIGITGGIAHNEDFVRRIRER
VEFIAPVYVYPGEDEMLALAEGAYRVLTGEENPKTYS

Sequences:

>Translated_357_residues
MGLALILVINPGSTSTKVAVFRDKEPVFTETLRHSTEELSKYKRIIDQFEFRTQAILDMLKEKGISLSQIDAIVGRGGLL
KPIESGTYIVNEKMLEDLKKAERGEHASNLGAIIAYTLAKEHNIPAYIVDPVVVDELEDVARITGLPEIEKQSIFHALNQ
KAIARRLASDLGKRYDEVNLIIAHLGGGISVGAHRKGRVIDVNDALNGEGPFSPERAGGLPVLDLVKLCYSGKYTFEEMK
KKLIGKGGIVAHLGTNDVREVYKMIENGDKNAELILDAMAYQTAKEIGSMAVVLKGKVDAIGITGGIAHNEDFVRRIRER
VEFIAPVYVYPGEDEMLALAEGAYRVLTGEENPKTYS
>Mature_356_residues
GLALILVINPGSTSTKVAVFRDKEPVFTETLRHSTEELSKYKRIIDQFEFRTQAILDMLKEKGISLSQIDAIVGRGGLLK
PIESGTYIVNEKMLEDLKKAERGEHASNLGAIIAYTLAKEHNIPAYIVDPVVVDELEDVARITGLPEIEKQSIFHALNQK
AIARRLASDLGKRYDEVNLIIAHLGGGISVGAHRKGRVIDVNDALNGEGPFSPERAGGLPVLDLVKLCYSGKYTFEEMKK
KLIGKGGIVAHLGTNDVREVYKMIENGDKNAELILDAMAYQTAKEIGSMAVVLKGKVDAIGITGGIAHNEDFVRRIRERV
EFIAPVYVYPGEDEMLALAEGAYRVLTGEENPKTYS

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI145693184, Length=165, Percent_Identity=25.4545454545455, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788633, Length=166, Percent_Identity=27.710843373494, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BUK1_THETN (Q8R832)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623752.1
- ProteinModelPortal:   Q8R832
- SMR:   Q8R832
- GeneID:   997632
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2201
- NMPDR:   fig|273068.3.peg.2385
- HOGENOM:   HBG449785
- OMA:   RSIFHAL
- ProtClustDB:   PRK03011
- BioCyc:   TTEN273068:TTE2201-MONOMER
- BRENDA:   2.7.2.7
- GO:   GO:0005737
- HAMAP:   MF_00542
- InterPro:   IPR000890
- InterPro:   IPR011245
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF3
- PIRSF:   PIRSF036458
- PRINTS:   PR00471
- TIGRFAMs:   TIGR02707

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.7

Molecular weight: Translated: 39218; Mature: 39087

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLALILVINPGSTSTKVAVFRDKEPVFTETLRHSTEELSKYKRIIDQFEFRTQAILDML
CCEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKGISLSQIDAIVGRGGLLKPIESGTYIVNEKMLEDLKKAERGEHASNLGAIIAYTLAK
HHCCCCHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EHNIPAYIVDPVVVDELEDVARITGLPEIEKQSIFHALNQKAIARRLASDLGKRYDEVNL
CCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
IIAHLGGGISVGAHRKGRVIDVNDALNGEGPFSPERAGGLPVLDLVKLCYSGKYTFEEMK
EEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KKLIGKGGIVAHLGTNDVREVYKMIENGDKNAELILDAMAYQTAKEIGSMAVVLKGKVDA
HHHCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCE
IGITGGIAHNEDFVRRIRERVEFIAPVYVYPGEDEMLALAEGAYRVLTGEENPKTYS
EEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
GLALILVINPGSTSTKVAVFRDKEPVFTETLRHSTEELSKYKRIIDQFEFRTQAILDML
CEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKGISLSQIDAIVGRGGLLKPIESGTYIVNEKMLEDLKKAERGEHASNLGAIIAYTLAK
HHCCCCHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EHNIPAYIVDPVVVDELEDVARITGLPEIEKQSIFHALNQKAIARRLASDLGKRYDEVNL
CCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
IIAHLGGGISVGAHRKGRVIDVNDALNGEGPFSPERAGGLPVLDLVKLCYSGKYTFEEMK
EEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KKLIGKGGIVAHLGTNDVREVYKMIENGDKNAELILDAMAYQTAKEIGSMAVVLKGKVDA
HHHCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCE
IGITGGIAHNEDFVRRIRERVEFIAPVYVYPGEDEMLALAEGAYRVLTGEENPKTYS
EEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336