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The map label for this gene is RpoD5 [H]

Identifier: 20808499

GI number: 20808499

Start: 2026723

End: 2027520

Strand: Reverse

Name: RpoD5 [H]

Synonym: TTE2104

Alternate gene names: 20808499

Gene position: 2027520-2026723 (Counterclockwise)

Preceding gene: 20808500

Following gene: 20808498

Centisome position: 75.39

GC content: 36.34

Gene sequence:

>798_bases
GTGAACAGCGTACTAACCAAAGAAAAAACCGATGAATTTGAACTTTTTGAACGGCTCAGAAATGGAGACAAAAAGGCCAG
AGATGAAATAGTTGAAAAAAACATAGGACTTGTTGTAAGTATTGCTGCTAATTTTTCAAAGGCTTATGGAGTAGAGCTGG
ATGATTTAATACAGGAAGGATTTATAGGCCTGCTTGCAGCGATAGAAGGTTTCGACCATACAAAGGGTTATAGATTTTCT
ACTTATGCAGTTTTTTACATAAAGCAGAAAATCCAGCGCTATATATGGGGACGTTCCAGAACAATACATTTGCCTGTAAA
AACCTATCAAAAGCTTAATCAGGTGTTGTCGGCTCAGCTAAAGCTTGAGCAGGTTAAAGGAGAAGCTTTTCCGGAAGAAA
TTGCTGAAGAAACAGGGATAAAAACAGAAGAAGTCGTAAATCTATTAAGCTTAAAAAATCCTACAGTAAGTTTAAATCAG
CAGCTGTATGAAAATGATAATACGGAACTGATAGATGTTATTCTGGATGAGCAAAGCGAAAATGAAATTGATAGAAATTA
TTCGTTAGAAGAGTTAAGAAAAATCTTAAAGGAATGTTTGGAGGTCCTACCTGAAAAAGAGCGCAGCGTTCTGGAAATGC
GTTACGGCTTAAACGGAACACCTATGACACTAAAACAAATAGCAGAAATTCTCAACATTTCCAAACAGCGTGCAGGACAG
ATCGAAAAAAGAGCTCTGCAGAGGTTGAGAAATAGCAGAAAAATTAGAAATGCCTTACTGGATTTTTTATATAACTAA

Upstream 100 bases:

>100_bases
GTTGGTATGGGAACTATGGAATTTGTCAAAATAGTTGTAGGGCAATAGATAGGAACAGGCGTTAAGCCTGTTTTTTTTAT
TTCAAGCGGGGTGATGATAT

Downstream 100 bases:

>100_bases
AATTCAAAAAGGGAGATGATAGATATGTATCAGTTTGCAGCAGGACTTGGAACAGGCCTTTTCATCGGTCTAATGTTCGG
GATAGGTTTTATGTGGTATC

Product: DNA-directed RNA polymerase sigma subunits (sigma70/sigma32)

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNSVLTKEKTDEFELFERLRNGDKKARDEIVEKNIGLVVSIAANFSKAYGVELDDLIQEGFIGLLAAIEGFDHTKGYRFS
TYAVFYIKQKIQRYIWGRSRTIHLPVKTYQKLNQVLSAQLKLEQVKGEAFPEEIAEETGIKTEEVVNLLSLKNPTVSLNQ
QLYENDNTELIDVILDEQSENEIDRNYSLEELRKILKECLEVLPEKERSVLEMRYGLNGTPMTLKQIAEILNISKQRAGQ
IEKRALQRLRNSRKIRNALLDFLYN

Sequences:

>Translated_265_residues
MNSVLTKEKTDEFELFERLRNGDKKARDEIVEKNIGLVVSIAANFSKAYGVELDDLIQEGFIGLLAAIEGFDHTKGYRFS
TYAVFYIKQKIQRYIWGRSRTIHLPVKTYQKLNQVLSAQLKLEQVKGEAFPEEIAEETGIKTEEVVNLLSLKNPTVSLNQ
QLYENDNTELIDVILDEQSENEIDRNYSLEELRKILKECLEVLPEKERSVLEMRYGLNGTPMTLKQIAEILNISKQRAGQ
IEKRALQRLRNSRKIRNALLDFLYN
>Mature_265_residues
MNSVLTKEKTDEFELFERLRNGDKKARDEIVEKNIGLVVSIAANFSKAYGVELDDLIQEGFIGLLAAIEGFDHTKGYRFS
TYAVFYIKQKIQRYIWGRSRTIHLPVKTYQKLNQVLSAQLKLEQVKGEAFPEEIAEETGIKTEEVVNLLSLKNPTVSLNQ
QLYENDNTELIDVILDEQSENEIDRNYSLEELRKILKECLEVLPEKERSVLEMRYGLNGTPMTLKQIAEILNISKQRAGQ
IEKRALQRLRNSRKIRNALLDFLYN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=34.156378600823, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789098, Length=251, Percent_Identity=34.2629482071713, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=32.512315270936, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789871, Length=253, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001909
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30688; Mature: 30688

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVLTKEKTDEFELFERLRNGDKKARDEIVEKNIGLVVSIAANFSKAYGVELDDLIQEG
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCCHHHHHHHH
FIGLLAAIEGFDHTKGYRFSTYAVFYIKQKIQRYIWGRSRTIHLPVKTYQKLNQVLSAQL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHH
KLEQVKGEAFPEEIAEETGIKTEEVVNLLSLKNPTVSLNQQLYENDNTELIDVILDEQSE
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEHHHHCCCCC
NEIDRNYSLEELRKILKECLEVLPEKERSVLEMRYGLNGTPMTLKQIAEILNISKQRAGQ
CHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IEKRALQRLRNSRKIRNALLDFLYN
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSVLTKEKTDEFELFERLRNGDKKARDEIVEKNIGLVVSIAANFSKAYGVELDDLIQEG
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCCHHHHHHHH
FIGLLAAIEGFDHTKGYRFSTYAVFYIKQKIQRYIWGRSRTIHLPVKTYQKLNQVLSAQL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHH
KLEQVKGEAFPEEIAEETGIKTEEVVNLLSLKNPTVSLNQQLYENDNTELIDVILDEQSE
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEHHHHCCCCC
NEIDRNYSLEELRKILKECLEVLPEKERSVLEMRYGLNGTPMTLKQIAEILNISKQRAGQ
CHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IEKRALQRLRNSRKIRNALLDFLYN
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA