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The map label for this gene is traC [H]

Identifier: 20808429

GI number: 20808429

Start: 1973105

End: 1973956

Strand: Reverse

Name: traC [H]

Synonym: TTE2027

Alternate gene names: 20808429

Gene position: 1973956-1973105 (Counterclockwise)

Preceding gene: 20808430

Following gene: 20808428

Centisome position: 73.4

GC content: 36.85

Gene sequence:

>852_bases
ATGAGCAAAGTCCACGAAATCGTACTTGAAAAAATTCTTCAACAGTTAGAAAGAGGAGAAATACCATGGAAAAAGGGTTG
GACAGAAAATCCTCCCATAAATTATATCACCAGGAAACCTTACACAGGAATAAACGTACTGCTGTTAAAAGGTGGAGAAT
ATCTCACCTTCAAGCAGATACAGCAGCTTGGGGGCAAAATAAAGAAAGGAGCAAAATCCGAAATAGTAGTATTTTTTAAG
CCTTACACCAAAATAGTCGAAGAAGTAGATTCAGAAACAGGCGAAGTTGTAGAGAAAGAAAAAGAATTGCTCGTTTTAAG
GTATTATCACGTATTTCACATAAACGATACAGAAGGAATTCCTTCCAAACTAAAAAAGGTAGAACATAATCCACTGCAGG
AAGCTGAAAAAATTATAGCAGGATACAAAAATGCCCCCAAAATAACCCATGATAACCCAAATGAAGCATACTACAACGCA
GCAAAAGATCTAATCAACATACCAGATAAAGGTTTTTTTTACAGCATACACGAATACTATTCTACGTTGTTCCATGAACT
TATTCACTCCACCGGCCATCCAAAGCGCTTAAACAGGTTCAAAGAAAATGAAAAAAGTGCTTTTGATGCAAATTCTTACT
CCTTAGAGGAACTGGTTGCAGAAATAGGCGCAGCAATGCTCTCTCAATATGCAGGGATTATGGAAAAAACAGTGGAAAAC
AATGCAGCATACATTCAGTCATGGTTAAAGCAGCTGCAGAACAATAAAATGATGATCTTTAAAGCAGCTGCGAAAGCACA
GCAGGCAGTAGACTACATCACTTTAAAAAAAGAGGAGTTATTATGCTATTAA

Upstream 100 bases:

>100_bases
AAGATGAAAGCAGTAAACCTAAGGAGGATTTTTTAGAAGACTTCTGGGACTTTGTTGCTGAAAACTTAAAAACCTATAAA
AGTAAAGGAGGAACAGGAGC

Downstream 100 bases:

>100_bases
ATTTTCCCCAAGACAAATATAAATTTTACAAAGACAAAAGTTAAAAAGATTACGTGGAGAAGGCTAGTTGCGCCTTCTCT
TTTTGTACATAGAGCTTTAT

Product: antirestriction protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSKVHEIVLEKILQQLERGEIPWKKGWTENPPINYITRKPYTGINVLLLKGGEYLTFKQIQQLGGKIKKGAKSEIVVFFK
PYTKIVEEVDSETGEVVEKEKELLVLRYYHVFHINDTEGIPSKLKKVEHNPLQEAEKIIAGYKNAPKITHDNPNEAYYNA
AKDLINIPDKGFFYSIHEYYSTLFHELIHSTGHPKRLNRFKENEKSAFDANSYSLEELVAEIGAAMLSQYAGIMEKTVEN
NAAYIQSWLKQLQNNKMMIFKAAAKAQQAVDYITLKKEELLCY

Sequences:

>Translated_283_residues
MSKVHEIVLEKILQQLERGEIPWKKGWTENPPINYITRKPYTGINVLLLKGGEYLTFKQIQQLGGKIKKGAKSEIVVFFK
PYTKIVEEVDSETGEVVEKEKELLVLRYYHVFHINDTEGIPSKLKKVEHNPLQEAEKIIAGYKNAPKITHDNPNEAYYNA
AKDLINIPDKGFFYSIHEYYSTLFHELIHSTGHPKRLNRFKENEKSAFDANSYSLEELVAEIGAAMLSQYAGIMEKTVEN
NAAYIQSWLKQLQNNKMMIFKAAAKAQQAVDYITLKKEELLCY
>Mature_282_residues
SKVHEIVLEKILQQLERGEIPWKKGWTENPPINYITRKPYTGINVLLLKGGEYLTFKQIQQLGGKIKKGAKSEIVVFFKP
YTKIVEEVDSETGEVVEKEKELLVLRYYHVFHINDTEGIPSKLKKVEHNPLQEAEKIIAGYKNAPKITHDNPNEAYYNAA
KDLINIPDKGFFYSIHEYYSTLFHELIHSTGHPKRLNRFKENEKSAFDANSYSLEELVAEIGAAMLSQYAGIMEKTVENN
AAYIQSWLKQLQNNKMMIFKAAAKAQQAVDYITLKKEELLCY

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 32619; Mature: 32488

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVHEIVLEKILQQLERGEIPWKKGWTENPPINYITRKPYTGINVLLLKGGEYLTFKQI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCEECHHHH
QQLGGKIKKGAKSEIVVFFKPYTKIVEEVDSETGEVVEKEKELLVLRYYHVFHINDTEGI
HHHHHHHHCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEEECCCCCC
PSKLKKVEHNPLQEAEKIIAGYKNAPKITHDNPNEAYYNAAKDLINIPDKGFFYSIHEYY
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
STLFHELIHSTGHPKRLNRFKENEKSAFDANSYSLEELVAEIGAAMLSQYAGIMEKTVEN
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NAAYIQSWLKQLQNNKMMIFKAAAKAQQAVDYITLKKEELLCY
CHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
SKVHEIVLEKILQQLERGEIPWKKGWTENPPINYITRKPYTGINVLLLKGGEYLTFKQI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCEECHHHH
QQLGGKIKKGAKSEIVVFFKPYTKIVEEVDSETGEVVEKEKELLVLRYYHVFHINDTEGI
HHHHHHHHCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEEECCCCCC
PSKLKKVEHNPLQEAEKIIAGYKNAPKITHDNPNEAYYNAAKDLINIPDKGFFYSIHEYY
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
STLFHELIHSTGHPKRLNRFKENEKSAFDANSYSLEELVAEIGAAMLSQYAGIMEKTVEN
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NAAYIQSWLKQLQNNKMMIFKAAAKAQQAVDYITLKKEELLCY
CHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]