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The map label for this gene is DnaG2 [H]

Identifier: 20808421

GI number: 20808421

Start: 1969474

End: 1970550

Strand: Reverse

Name: DnaG2 [H]

Synonym: TTE2018

Alternate gene names: 20808421

Gene position: 1970550-1969474 (Counterclockwise)

Preceding gene: 20808422

Following gene: 20808420

Centisome position: 73.27

GC content: 40.67

Gene sequence:

>1077_bases
ATGCACAGAGATTTGGACATTTTGGACGTTGCAAAAAGGTTGGGGATAAGAATTCTAAGAAGAGCATCAAATGAAGAATA
CATTGCGAGGTGTCCGTTCTGCGGAGATTCAAAAAAGGACCCTGAACACGGACACCTTTATTTAAATGTATCAAAAAATG
TCTACTACTGTGTACGCTGCGGGGAAGGCGGGGATGCAGTAGACCTGTATGCGAAACTTGAAAATATAAGTAGAAAAGAG
GCATACAAAAGAATAAAAGAAGAAAATTACCCTACAAATCCTGCTGCCAAGGAAAGAGCTGACAAAAGGATAAAATATAA
CTCAGTCGCACCTATAGAAACCAGAGATAAAGTATACAGGGCCTTTTTAGATAAACTTGTCTTGGAACCAGAACACCGAA
AAAAACTTCTTAAAAGGGGCCTATCCTGGGAAGAAACAGTCAAAAACCTTTATAAGTCCCTACCGGAAGAACCGCAGCAA
CGGTGGGAAATATGTAAGGAACTTATCAAAGAAGGGTATAACCTAAAAGGCATTCCGGGCTTTTACCAGAGAGAAAAGGA
TGGGGAAAGGTACTGGGACTTTGTAGATTATAAAGGATTTTTAATCCCTGTAAAAGACGTACAGGGACGAATACAGGGCT
TTCAAATAAGACTGGACGAAGAAGAGTTCGGAAAATATGTCTGGTTCTCGTCCAGAAATAAATTAAACGGCACACCTGCT
CATGCGTGGCAGGGCGTACATGGAGAGCCTTCTAAAGTTGTTATTGTTACTGAAGGCCCTTTAAAAGCTGATGTGGCGCA
TTATCTGTCACGTTTTACGTTTGTGTCCGTACCCGGAGTTACAGCGATAAAAGGTATAGAGGTTGTGCTAAAGCAGTTGG
GAGCAGAAAAAATCTACATTGCATTTGACATGGACAGTTTAACAAACAAAGCAGTGCAAAAGGCAAAAGAAAAGCTGGAA
AAGAAGCTTACTGAAGCAGGCTTTGTAGTTAAAACAAAAACATGGGACAGCAGGTATAAAGGTATTGATGACTACCTTCT
AGCGCAGAGAAAGCAAAAACAAAAGGAAGTTGTGTGA

Upstream 100 bases:

>100_bases
ACAAACTGGTACTAATTCCAAGATGGCCTTACAATCTAGACTTATTTGAAACCTTTGAAAATGCTGTCTATTACAACATT
TAAAAGGAGGAGGACTTAAA

Downstream 100 bases:

>100_bases
GAGAGGCTTTTGGCCTCTCTCTTTTTAAATTTTGAAGGAGGGGAATTAAGTTGGACAAAAACATAAAATTAATAGAGTTA
TGGGGAAATGAAATCTGGAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MHRDLDILDVAKRLGIRILRRASNEEYIARCPFCGDSKKDPEHGHLYLNVSKNVYYCVRCGEGGDAVDLYAKLENISRKE
AYKRIKEENYPTNPAAKERADKRIKYNSVAPIETRDKVYRAFLDKLVLEPEHRKKLLKRGLSWEETVKNLYKSLPEEPQQ
RWEICKELIKEGYNLKGIPGFYQREKDGERYWDFVDYKGFLIPVKDVQGRIQGFQIRLDEEEFGKYVWFSSRNKLNGTPA
HAWQGVHGEPSKVVIVTEGPLKADVAHYLSRFTFVSVPGVTAIKGIEVVLKQLGAEKIYIAFDMDSLTNKAVQKAKEKLE
KKLTEAGFVVKTKTWDSRYKGIDDYLLAQRKQKQKEVV

Sequences:

>Translated_358_residues
MHRDLDILDVAKRLGIRILRRASNEEYIARCPFCGDSKKDPEHGHLYLNVSKNVYYCVRCGEGGDAVDLYAKLENISRKE
AYKRIKEENYPTNPAAKERADKRIKYNSVAPIETRDKVYRAFLDKLVLEPEHRKKLLKRGLSWEETVKNLYKSLPEEPQQ
RWEICKELIKEGYNLKGIPGFYQREKDGERYWDFVDYKGFLIPVKDVQGRIQGFQIRLDEEEFGKYVWFSSRNKLNGTPA
HAWQGVHGEPSKVVIVTEGPLKADVAHYLSRFTFVSVPGVTAIKGIEVVLKQLGAEKIYIAFDMDSLTNKAVQKAKEKLE
KKLTEAGFVVKTKTWDSRYKGIDDYLLAQRKQKQKEVV
>Mature_358_residues
MHRDLDILDVAKRLGIRILRRASNEEYIARCPFCGDSKKDPEHGHLYLNVSKNVYYCVRCGEGGDAVDLYAKLENISRKE
AYKRIKEENYPTNPAAKERADKRIKYNSVAPIETRDKVYRAFLDKLVLEPEHRKKLLKRGLSWEETVKNLYKSLPEEPQQ
RWEICKELIKEGYNLKGIPGFYQREKDGERYWDFVDYKGFLIPVKDVQGRIQGFQIRLDEEEFGKYVWFSSRNKLNGTPA
HAWQGVHGEPSKVVIVTEGPLKADVAHYLSRFTFVSVPGVTAIKGIEVVLKQLGAEKIYIAFDMDSLTNKAVQKAKEKLE
KKLTEAGFVVKTKTWDSRYKGIDDYLLAQRKQKQKEVV

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 41513; Mature: 41513

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRDLDILDVAKRLGIRILRRASNEEYIARCPFCGDSKKDPEHGHLYLNVSKNVYYCVRC
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEE
GEGGDAVDLYAKLENISRKEAYKRIKEENYPTNPAAKERADKRIKYNSVAPIETRDKVYR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
AFLDKLVLEPEHRKKLLKRGLSWEETVKNLYKSLPEEPQQRWEICKELIKEGYNLKGIPG
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
FYQREKDGERYWDFVDYKGFLIPVKDVQGRIQGFQIRLDEEEFGKYVWFSSRNKLNGTPA
HHHCCCCCCHHHHEECCCCEEEEHHHHHCCCEEEEEEECHHHHCCEEEEECCCCCCCCCH
HAWQGVHGEPSKVVIVTEGPLKADVAHYLSRFTFVSVPGVTAIKGIEVVLKQLGAEKIYI
HHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEE
AFDMDSLTNKAVQKAKEKLEKKLTEAGFVVKTKTWDSRYKGIDDYLLAQRKQKQKEVV
EEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHRDLDILDVAKRLGIRILRRASNEEYIARCPFCGDSKKDPEHGHLYLNVSKNVYYCVRC
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEE
GEGGDAVDLYAKLENISRKEAYKRIKEENYPTNPAAKERADKRIKYNSVAPIETRDKVYR
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
AFLDKLVLEPEHRKKLLKRGLSWEETVKNLYKSLPEEPQQRWEICKELIKEGYNLKGIPG
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
FYQREKDGERYWDFVDYKGFLIPVKDVQGRIQGFQIRLDEEEFGKYVWFSSRNKLNGTPA
HHHCCCCCCHHHHEECCCCEEEEHHHHHCCCEEEEEEECHHHHCCEEEEECCCCCCCCCH
HAWQGVHGEPSKVVIVTEGPLKADVAHYLSRFTFVSVPGVTAIKGIEVVLKQLGAEKIYI
HHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEE
AFDMDSLTNKAVQKAKEKLEKKLTEAGFVVKTKTWDSRYKGIDDYLLAQRKQKQKEVV
EEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]