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The map label for this gene is PurR8 [H]

Identifier: 20808356

GI number: 20808356

Start: 1898747

End: 1899742

Strand: Reverse

Name: PurR8 [H]

Synonym: TTE1952

Alternate gene names: 20808356

Gene position: 1899742-1898747 (Counterclockwise)

Preceding gene: 20808357

Following gene: 20808355

Centisome position: 70.64

GC content: 40.86

Gene sequence:

>996_bases
ATGGCTAATATAAGAGAGGTTGCAAAGAGGGCAGGGGTTTCTGTTGCCACAGTCTCAAGAGTTTTAAATAACAGCGATTT
GGTGTCAGAAGAAACACGTGAGAGGGTTTTGAAAGCCATAAAAGAGCTAAATTACCATCCCAATCTTCTTGGCAGAAATT
TAAGGCGCTTAAAAACGAAAATGGTACTGGCACTTATGCCAAACGTGTCGAACCCCTTTTATGCAAGAGTTGTAAAGGGA
ATAGAAGATGTAGCTCATAAAAATGGGTACAATGTAATGCTTTGCAACACAGATTCAGACCCTGAGAGGGAAAAAGCGTA
TTTGGACCTTTTGAAAAATAAGCTGGCAGATGGAGTCATCCTCATGGCATCAGTTATGGGAAAAGACGAGTTAACTCTTC
TGGCTAAAAATTACCCTGTAGTTCAATGCTGTGAATACATAGAAGGAGCGGGTGCATCTTACGTCTCTATAGACAATTTT
TCTGCTGCCTATAAAGCTGTAAAGCACCTTATAAATTTGGGCCTCAGGAGGATAGCACTGATTACCTGTAAAAACGACTT
TATTTCCACCAGGCAAAGAGAAACAGGATACAGAAAGGCCCTGGAGGATGCGAATATAGAATTTGATGAAAATTTGATAA
AATATGGAGATTACAGTTTTAAAAGCGGGATGAGAGCGGCCAGACAGCTTTTATCTTTAGAAGAAAAGCCTACTGCCATA
TTTGCAATTTCTGACATAATGGCCATCGGAGCTATAAAGGCTTTAAAAGAAGAGGGATTTAAGGTTCCAGAAGATGTAGC
AGTTGTAGGATTTGACGATATAAGCTTTGCCTCCATGTACGACCCGGCCCTTACCACGGTATCTCAGCCCAAATACGATT
TGGGATGTATCGCGATGGAAGTCCTCTTAAAGCATATTGAAGGGGAACTGGAAGATCCGCAGAGAATTTTTCTGGAGCAC
GAGCTTGTTATAAGAGAGTCAACAGTCAAGTATTAA

Upstream 100 bases:

>100_bases
TAGTGGTATAATAGTTAATGTAATGCGTTACAAAATTGCAGAAAAGATGGTTGAAATTTATTGAAAAATATTATGTAATC
TGTTACATAGGAGGCTTGCG

Downstream 100 bases:

>100_bases
AAATTGCCCTTTGAGGCAATTTTCAAAATAAACAGCAAAGGGAGGAAGAAAAATGTCCAAGGAAAAAGTGAGAGTAGGAA
TAATTGGTTGCGGAGGGATA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MANIREVAKRAGVSVATVSRVLNNSDLVSEETRERVLKAIKELNYHPNLLGRNLRRLKTKMVLALMPNVSNPFYARVVKG
IEDVAHKNGYNVMLCNTDSDPEREKAYLDLLKNKLADGVILMASVMGKDELTLLAKNYPVVQCCEYIEGAGASYVSIDNF
SAAYKAVKHLINLGLRRIALITCKNDFISTRQRETGYRKALEDANIEFDENLIKYGDYSFKSGMRAARQLLSLEEKPTAI
FAISDIMAIGAIKALKEEGFKVPEDVAVVGFDDISFASMYDPALTTVSQPKYDLGCIAMEVLLKHIEGELEDPQRIFLEH
ELVIRESTVKY

Sequences:

>Translated_331_residues
MANIREVAKRAGVSVATVSRVLNNSDLVSEETRERVLKAIKELNYHPNLLGRNLRRLKTKMVLALMPNVSNPFYARVVKG
IEDVAHKNGYNVMLCNTDSDPEREKAYLDLLKNKLADGVILMASVMGKDELTLLAKNYPVVQCCEYIEGAGASYVSIDNF
SAAYKAVKHLINLGLRRIALITCKNDFISTRQRETGYRKALEDANIEFDENLIKYGDYSFKSGMRAARQLLSLEEKPTAI
FAISDIMAIGAIKALKEEGFKVPEDVAVVGFDDISFASMYDPALTTVSQPKYDLGCIAMEVLLKHIEGELEDPQRIFLEH
ELVIRESTVKY
>Mature_330_residues
ANIREVAKRAGVSVATVSRVLNNSDLVSEETRERVLKAIKELNYHPNLLGRNLRRLKTKMVLALMPNVSNPFYARVVKGI
EDVAHKNGYNVMLCNTDSDPEREKAYLDLLKNKLADGVILMASVMGKDELTLLAKNYPVVQCCEYIEGAGASYVSIDNFS
AAYKAVKHLINLGLRRIALITCKNDFISTRQRETGYRKALEDANIEFDENLIKYGDYSFKSGMRAARQLLSLEEKPTAIF
AISDIMAIGAIKALKEEGFKVPEDVAVVGFDDISFASMYDPALTTVSQPKYDLGCIAMEVLLKHIEGELEDPQRIFLEHE
LVIRESTVKY

Specific function: Involved in the regulation of amylase production [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=38.109756097561, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=37.5757575757576, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=37.5757575757576, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788474, Length=330, Percent_Identity=34.2424242424242, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=32.7327327327327, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=33.9041095890411, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787580, Length=331, Percent_Identity=30.5135951661631, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=30.3125, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=30.6060606060606, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=27.6527331189711, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789456, Length=344, Percent_Identity=27.6162790697674, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787906, Length=338, Percent_Identity=27.2189349112426, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=22.5396825396825, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=320, Percent_Identity=24.0625, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37079; Mature: 36948

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIREVAKRAGVSVATVSRVLNNSDLVSEETRERVLKAIKELNYHPNLLGRNLRRLKTK
CCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MVLALMPNVSNPFYARVVKGIEDVAHKNGYNVMLCNTDSDPEREKAYLDLLKNKLADGVI
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHH
LMASVMGKDELTLLAKNYPVVQCCEYIEGAGASYVSIDNFSAAYKAVKHLINLGLRRIAL
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEE
ITCKNDFISTRQRETGYRKALEDANIEFDENLIKYGDYSFKSGMRAARQLLSLEEKPTAI
EEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEE
FAISDIMAIGAIKALKEEGFKVPEDVAVVGFDDISFASMYDPALTTVSQPKYDLGCIAME
EHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCCCHHHHHHHH
VLLKHIEGELEDPQRIFLEHELVIRESTVKY
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANIREVAKRAGVSVATVSRVLNNSDLVSEETRERVLKAIKELNYHPNLLGRNLRRLKTK
CCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MVLALMPNVSNPFYARVVKGIEDVAHKNGYNVMLCNTDSDPEREKAYLDLLKNKLADGVI
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHH
LMASVMGKDELTLLAKNYPVVQCCEYIEGAGASYVSIDNFSAAYKAVKHLINLGLRRIAL
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEE
ITCKNDFISTRQRETGYRKALEDANIEFDENLIKYGDYSFKSGMRAARQLLSLEEKPTAI
EEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEE
FAISDIMAIGAIKALKEEGFKVPEDVAVVGFDDISFASMYDPALTTVSQPKYDLGCIAME
EHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCCCHHHHHHHH
VLLKHIEGELEDPQRIFLEHELVIRESTVKY
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7539689 [H]