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The map label for this gene is AmyA [C]

Identifier: 20808241

GI number: 20808241

Start: 1774035

End: 1775759

Strand: Reverse

Name: AmyA [C]

Synonym: TTE1825

Alternate gene names: 20808241

Gene position: 1775759-1774035 (Counterclockwise)

Preceding gene: 20808242

Following gene: 20808240

Centisome position: 66.03

GC content: 38.96

Gene sequence:

>1725_bases
ATGATAAAAGAGGCTATATTCCACAAAAGCGATATACCTTATGCATATCCTGTAAATAAAGACCAATTAAAAATAGTCTT
GAGAACGGCAGTTTTCGATGTGGATAGGGTATACATTCTTTTTAAAGACAGGTATGACTGGCTGGGCAAATTTAAAATAA
AGCCTATGACCTTAGTTCACACTAACGAGCTTTTTGACTATTATGAGACTACTCTTGAATTAAATAAGAGGTTTTTGTAC
TTTTTTTATTTAGTTTCAGAAACGGGAGAAAAACTTTATTACACAGAAGCTGGTTTTTTCAAAAAGAGGCCAGAGGATAG
CTTCTGGGGTTTTTTCCACTATCCCTATATAAGGGAAAAGGATATATTTTCTCCTCCGGATTGGGCAAACGAGTGCATAG
TGTATCAGATATTTCCAGACAGATTCAACAATGGAGACAGAACTAATGACCCCGAAAACGTAAAGCCATGGGGGGAAAAG
CCGACTGCAGATTCTTTTTTTGGAGGGGATTTACAGGGAATAATCGACAAAATAGATTATTTAAAAGAGTTAGGGATAAA
TGCCATTTATTTAACTCCAATATTTTTGTCTCCTTCTACTCACAAATACGACACCACGGATTATTACACGATAGACCCTC
ATTTTGGTGACACGGAAAAAGCTAAAGAATTGGTGAGGAAGTGTCATGACAATGGCATAAAAGTTATATTCGATGCAGTG
TTCAACCACTGTGGATACGACTTTTTTGCATTTCAGGATGTGGTTAAAAATGGGCCGAAGTCAAAGTACTGGGATTGGTT
CAATGTGTACGAATGGCCCATTAAAACGCATCCCAAACCTACTTACGAGACTTTTGGCGAGAATGTGTGGAGAATGCCTA
AGCTTATGACTAAAAACCCGGAAGTGCAAAAGTACCTACTGGAAGTGGCTGAATACTGGATTAAAGAAGTAGACATTGAC
GGCTGGCGATTGGACGTTGCCAACGAAATAGACCATCACTTCTGGAGAAAGTTTAGAGAGGTTGTAAAAAATGCCAAAAA
AGATGCCATAATAGTAGGAGAAGTGTGGCATGATGCTTCTCCGTGGTTAAGAGGGGACCAGTTTGACTCTGTCATGAATT
ATCCTTTTAGAAATGCAGTTGTAGACTTTTTTGCGAAGAGGAAAATAAGTGCTGTAAAATTCAACACACTGATAACTGAG
CAGCTTATGAGACACATGGATAGCGTTAATAGAGTCATGTTTAACCTCATAGGTAGTCACGACACTGAAAGGTTTTTGAC
TCTCGCAAATGGCATGGTGGCGCGGATGAAATTAGCTTTGGTTTTCCAGTTTACATTTGTGGGAGTGCCGTACATCTACT
ATGGAGACGAAGCTGGAATGGTGGGAGGATTTGACCCTGACTGCAGAAGGTGCATGATATGGGAGGAGGAAAAGCAGAAC
AAGGGACTTTTTGAGTTTTACAAAAAGCTCATCCGCATAAGAAAGGAGCATGAAGAACTTAAGTATGGCAATTTCACCAC
TTTGTACGCTGTTGGAAGGGTGTTTGCTTTTAGAAGGGAATATAAAGGAAATTCAATTGTGGTGATTATAAACAACAGCA
GCAAAGAGGAAGTGATATTTTTAGAAGAGGCGGGAGGAAAAGAGGATATATTAAAAATGGGAGAATTAAAAAGGAGTGGC
AATTTGCTGTATCTTAAGCCTAACACTGCCTATATTTTGAAGTAG

Upstream 100 bases:

>100_bases
CCACCAGCTGTTAAAATAAGTGTAGATGGTGAAGAACTGCCAGCTGTAAGTAGTCCCTCTCCAGTTACTATAAACATACA
CACGTAGAAAGGGGAAAGAT

Downstream 100 bases:

>100_bases
CAAATTAAAGGTTATGTGAATAATATGTACAGTAGAAAATAGAGGGGAATCTTAATTCCCTTCTATTTTTTAGAAAACAG
GGGTGATGTTATGAAAAATA

Product: glycosidases

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MIKEAIFHKSDIPYAYPVNKDQLKIVLRTAVFDVDRVYILFKDRYDWLGKFKIKPMTLVHTNELFDYYETTLELNKRFLY
FFYLVSETGEKLYYTEAGFFKKRPEDSFWGFFHYPYIREKDIFSPPDWANECIVYQIFPDRFNNGDRTNDPENVKPWGEK
PTADSFFGGDLQGIIDKIDYLKELGINAIYLTPIFLSPSTHKYDTTDYYTIDPHFGDTEKAKELVRKCHDNGIKVIFDAV
FNHCGYDFFAFQDVVKNGPKSKYWDWFNVYEWPIKTHPKPTYETFGENVWRMPKLMTKNPEVQKYLLEVAEYWIKEVDID
GWRLDVANEIDHHFWRKFREVVKNAKKDAIIVGEVWHDASPWLRGDQFDSVMNYPFRNAVVDFFAKRKISAVKFNTLITE
QLMRHMDSVNRVMFNLIGSHDTERFLTLANGMVARMKLALVFQFTFVGVPYIYYGDEAGMVGGFDPDCRRCMIWEEEKQN
KGLFEFYKKLIRIRKEHEELKYGNFTTLYAVGRVFAFRREYKGNSIVVIINNSSKEEVIFLEEAGGKEDILKMGELKRSG
NLLYLKPNTAYILK

Sequences:

>Translated_574_residues
MIKEAIFHKSDIPYAYPVNKDQLKIVLRTAVFDVDRVYILFKDRYDWLGKFKIKPMTLVHTNELFDYYETTLELNKRFLY
FFYLVSETGEKLYYTEAGFFKKRPEDSFWGFFHYPYIREKDIFSPPDWANECIVYQIFPDRFNNGDRTNDPENVKPWGEK
PTADSFFGGDLQGIIDKIDYLKELGINAIYLTPIFLSPSTHKYDTTDYYTIDPHFGDTEKAKELVRKCHDNGIKVIFDAV
FNHCGYDFFAFQDVVKNGPKSKYWDWFNVYEWPIKTHPKPTYETFGENVWRMPKLMTKNPEVQKYLLEVAEYWIKEVDID
GWRLDVANEIDHHFWRKFREVVKNAKKDAIIVGEVWHDASPWLRGDQFDSVMNYPFRNAVVDFFAKRKISAVKFNTLITE
QLMRHMDSVNRVMFNLIGSHDTERFLTLANGMVARMKLALVFQFTFVGVPYIYYGDEAGMVGGFDPDCRRCMIWEEEKQN
KGLFEFYKKLIRIRKEHEELKYGNFTTLYAVGRVFAFRREYKGNSIVVIINNSSKEEVIFLEEAGGKEDILKMGELKRSG
NLLYLKPNTAYILK
>Mature_574_residues
MIKEAIFHKSDIPYAYPVNKDQLKIVLRTAVFDVDRVYILFKDRYDWLGKFKIKPMTLVHTNELFDYYETTLELNKRFLY
FFYLVSETGEKLYYTEAGFFKKRPEDSFWGFFHYPYIREKDIFSPPDWANECIVYQIFPDRFNNGDRTNDPENVKPWGEK
PTADSFFGGDLQGIIDKIDYLKELGINAIYLTPIFLSPSTHKYDTTDYYTIDPHFGDTEKAKELVRKCHDNGIKVIFDAV
FNHCGYDFFAFQDVVKNGPKSKYWDWFNVYEWPIKTHPKPTYETFGENVWRMPKLMTKNPEVQKYLLEVAEYWIKEVDID
GWRLDVANEIDHHFWRKFREVVKNAKKDAIIVGEVWHDASPWLRGDQFDSVMNYPFRNAVVDFFAKRKISAVKFNTLITE
QLMRHMDSVNRVMFNLIGSHDTERFLTLANGMVARMKLALVFQFTFVGVPYIYYGDEAGMVGGFDPDCRRCMIWEEEKQN
KGLFEFYKKLIRIRKEHEELKYGNFTTLYAVGRVFAFRREYKGNSIVVIINNSSKEEVIFLEEAGGKEDILKMGELKRSG
NLLYLKPNTAYILK

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=393, Percent_Identity=24.6819338422392, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1786604, Length=466, Percent_Identity=34.7639484978541, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1789995, Length=511, Percent_Identity=23.091976516634, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790687, Length=528, Percent_Identity=24.2424242424242, Blast_Score=118, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6322245, Length=230, Percent_Identity=30.8695652173913, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321731, Length=412, Percent_Identity=25, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=412, Percent_Identity=24.7572815533981, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321726, Length=352, Percent_Identity=25.5681818181818, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324416, Length=313, Percent_Identity=27.4760383386581, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322241, Length=351, Percent_Identity=26.7806267806268, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322021, Length=351, Percent_Identity=26.7806267806268, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586587, Length=228, Percent_Identity=31.140350877193, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583745, Length=601, Percent_Identity=23.1281198003328, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330053, Length=221, Percent_Identity=28.9592760180996, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583747, Length=230, Percent_Identity=29.5652173913043, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583749, Length=230, Percent_Identity=29.5652173913043, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586599, Length=223, Percent_Identity=27.3542600896861, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24586597, Length=223, Percent_Identity=26.9058295964126, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24586591, Length=406, Percent_Identity=22.9064039408867, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24586589, Length=217, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586593, Length=240, Percent_Identity=25.8333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45549022, Length=134, Percent_Identity=32.089552238806, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 68002; Mature: 68002

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEAIFHKSDIPYAYPVNKDQLKIVLRTAVFDVDRVYILFKDRYDWLGKFKIKPMTLVH
CCCHHHHCCCCCCEECCCCHHHHHEEHHHHHHCCCEEEEEEECCCCCCCEEEECEEEEEE
TNELFDYYETTLELNKRFLYFFYLVSETGEKLYYTEAGFFKKRPEDSFWGFFHYPYIREK
HHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCC
DIFSPPDWANECIVYQIFPDRFNNGDRTNDPENVKPWGEKPTADSFFGGDLQGIIDKIDY
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LKELGINAIYLTPIFLSPSTHKYDTTDYYTIDPHFGDTEKAKELVRKCHDNGIKVIFDAV
HHHCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEHHHHH
FNHCGYDFFAFQDVVKNGPKSKYWDWFNVYEWPIKTHPKPTYETFGENVWRMPKLMTKNP
HHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCHHHHCCHHHCCCH
EVQKYLLEVAEYWIKEVDIDGWRLDVANEIDHHFWRKFREVVKNAKKDAIIVGEVWHDAS
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
PWLRGDQFDSVMNYPFRNAVVDFFAKRKISAVKFNTLITEQLMRHMDSVNRVMFNLIGSH
CCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTERFLTLANGMVARMKLALVFQFTFVGVPYIYYGDEAGMVGGFDPDCRRCMIWEEEKQN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCC
KGLFEFYKKLIRIRKEHEELKYGNFTTLYAVGRVFAFRREYKGNSIVVIINNSSKEEVIF
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEE
LEEAGGKEDILKMGELKRSGNLLYLKPNTAYILK
EECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEC
>Mature Secondary Structure
MIKEAIFHKSDIPYAYPVNKDQLKIVLRTAVFDVDRVYILFKDRYDWLGKFKIKPMTLVH
CCCHHHHCCCCCCEECCCCHHHHHEEHHHHHHCCCEEEEEEECCCCCCCEEEECEEEEEE
TNELFDYYETTLELNKRFLYFFYLVSETGEKLYYTEAGFFKKRPEDSFWGFFHYPYIREK
HHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCC
DIFSPPDWANECIVYQIFPDRFNNGDRTNDPENVKPWGEKPTADSFFGGDLQGIIDKIDY
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LKELGINAIYLTPIFLSPSTHKYDTTDYYTIDPHFGDTEKAKELVRKCHDNGIKVIFDAV
HHHCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEHHHHH
FNHCGYDFFAFQDVVKNGPKSKYWDWFNVYEWPIKTHPKPTYETFGENVWRMPKLMTKNP
HHHCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCHHHHCCHHHCCCH
EVQKYLLEVAEYWIKEVDIDGWRLDVANEIDHHFWRKFREVVKNAKKDAIIVGEVWHDAS
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
PWLRGDQFDSVMNYPFRNAVVDFFAKRKISAVKFNTLITEQLMRHMDSVNRVMFNLIGSH
CCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTERFLTLANGMVARMKLALVFQFTFVGVPYIYYGDEAGMVGGFDPDCRRCMIWEEEKQN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCC
KGLFEFYKKLIRIRKEHEELKYGNFTTLYAVGRVFAFRREYKGNSIVVIINNSSKEEVIF
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEE
LEEAGGKEDILKMGELKRSGNLLYLKPNTAYILK
EECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]