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The map label for this gene is Nth [H]

Identifier: 20808188

GI number: 20808188

Start: 1716324

End: 1716965

Strand: Reverse

Name: Nth [H]

Synonym: TTE1769

Alternate gene names: 20808188

Gene position: 1716965-1716324 (Counterclockwise)

Preceding gene: 20808189

Following gene: 20808187

Centisome position: 63.84

GC content: 38.01

Gene sequence:

>642_bases
GTGCGCATATCTAAAGAAGAAGCCCTGAAAGTCATAGAGATTTTGAAAAAAATCTATCCAAATGCAAAATCTGGTCTTAA
GTTTAATAATCCTTTTGAGCTTTTGATTGCCACTATTTTGTCTGCTCAGTGCACTGACAAGAGGGTAAATATAATCACTG
AGAGGCTTTTTAAAAAGTACAAGACTCCAGAGGATTTTTTAAAATTGACTCCTGAAGAACTTCAGGAGGAGATAAGGGAG
TGTGGCCTTTACAGGAATAAGTCCAAAAGCATACTTGAGACCTGCAGAATTTTGAAAGAAAAGCATAACGGAAAAGTGCC
TGATACTTTGGAAGAACTAATGGCTTTACCAGGGGTAGGAAGAAAGACTGCCAATGTGGTTTTAAGCAATGCCTTCTCAA
AAGATGCCATAGCTGTGGATACCCATGTATTTAGAGTTTCAAACAGAATAGGGCTGGCAGATAGCAAGGATGTTTTGACA
ACTGAGAAACAGCTCATGGAAATTATACCGAAAAATTTGTGGTCAATATCCCACCACCTTTTGATATATCACGGCAGAAA
TTTATGTACTGCTAGAAAGCCTAAGTGCGACAAGTGTCCAGTAAAGGAGTTTTGCCTTTACTTTAAGGGGAGAAAAATTT
AA

Upstream 100 bases:

>100_bases
AAGCTATAGAGGAAATAAAAGAAGCTTTGAATAAAGAAAAAGATGGTTTTGCAATAAAGATGATGGCAGAAGCCTTACAA
AAACTAGAAAGGTGAGGAAA

Downstream 100 bases:

>100_bases
TGTTTTTGTAATTTTTTTAAGGAATAATTAATTGTATTGGAAGAGAAATTGTCATATATTAAAAAAAGAGACCAAAATTA
AAAATGGGAGAGAAATGGAT

Product: EndoIII-related endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRISKEEALKVIEILKKIYPNAKSGLKFNNPFELLIATILSAQCTDKRVNIITERLFKKYKTPEDFLKLTPEELQEEIRE
CGLYRNKSKSILETCRILKEKHNGKVPDTLEELMALPGVGRKTANVVLSNAFSKDAIAVDTHVFRVSNRIGLADSKDVLT
TEKQLMEIIPKNLWSISHHLLIYHGRNLCTARKPKCDKCPVKEFCLYFKGRKI

Sequences:

>Translated_213_residues
MRISKEEALKVIEILKKIYPNAKSGLKFNNPFELLIATILSAQCTDKRVNIITERLFKKYKTPEDFLKLTPEELQEEIRE
CGLYRNKSKSILETCRILKEKHNGKVPDTLEELMALPGVGRKTANVVLSNAFSKDAIAVDTHVFRVSNRIGLADSKDVLT
TEKQLMEIIPKNLWSISHHLLIYHGRNLCTARKPKCDKCPVKEFCLYFKGRKI
>Mature_213_residues
MRISKEEALKVIEILKKIYPNAKSGLKFNNPFELLIATILSAQCTDKRVNIITERLFKKYKTPEDFLKLTPEELQEEIRE
CGLYRNKSKSILETCRILKEKHNGKVPDTLEELMALPGVGRKTANVVLSNAFSKDAIAVDTHVFRVSNRIGLADSKDVLT
TEKQLMEIIPKNLWSISHHLLIYHGRNLCTARKPKCDKCPVKEFCLYFKGRKI

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=31.8681318681319, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=46.3414634146341, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1789331, Length=193, Percent_Identity=26.4248704663212, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=36.094674556213, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=30.5263157894737, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=35.0282485875706, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24458; Mature: 24458

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISKEEALKVIEILKKIYPNAKSGLKFNNPFELLIATILSAQCTDKRVNIITERLFKKY
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KTPEDFLKLTPEELQEEIRECGLYRNKSKSILETCRILKEKHNGKVPDTLEELMALPGVG
CCHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
RKTANVVLSNAFSKDAIAVDTHVFRVSNRIGLADSKDVLTTEKQLMEIIPKNLWSISHHL
CHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
LIYHGRNLCTARKPKCDKCPVKEFCLYFKGRKI
EEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MRISKEEALKVIEILKKIYPNAKSGLKFNNPFELLIATILSAQCTDKRVNIITERLFKKY
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KTPEDFLKLTPEELQEEIRECGLYRNKSKSILETCRILKEKHNGKVPDTLEELMALPGVG
CCHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
RKTANVVLSNAFSKDAIAVDTHVFRVSNRIGLADSKDVLTTEKQLMEIIPKNLWSISHHL
CHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
LIYHGRNLCTARKPKCDKCPVKEFCLYFKGRKI
EEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]