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The map label for this gene is RpoN [H]

Identifier: 20808183

GI number: 20808183

Start: 1711533

End: 1712888

Strand: Reverse

Name: RpoN [H]

Synonym: TTE1764

Alternate gene names: 20808183

Gene position: 1712888-1711533 (Counterclockwise)

Preceding gene: 20808184

Following gene: 20808182

Centisome position: 63.69

GC content: 38.57

Gene sequence:

>1356_bases
ATGAGGATGGAATTTGATCTGAAACTTCAGCAAACCCAAAAGCTCATAATGACTCCAGAGCTAAAACAGGCTATTGAAAT
TTTGCAGTTAAATGCTCTGGAGTTAAATGAATTGATACAGCAGGAACTGGAGAATAATCCCCTTCTTGAGAGAGAAGAGG
TTGAAGAAGATATATACGTGGTAGATGTCAAAGAATTGGCCAAAAGCATAAGGGAAATGGAAAAGAGGATGTACTACAGG
GATTCTGATGATGAGGAAATGGATGAATTCAATTATGAGAATTTTGTGTCTGCTAAACCAGGCTTAAGCGATCATCTTCT
TTTTCAACTTCATATAACACCTGTTCCTACTAGGATTCGCAAGATATGCGAGTATATAATATTTAGCCTAACTCCCAGTG
GTTATTTAAAAGAAGATGTAAGAGAAATTGCACAGATGCTGGAGTGCACTGAAGAAGAGGTTTTGGAGGCATTGGCTGTT
GTGCAATCTTTTGACCCCCCTGGTGTAGGTGCCCGCAATTTGCAGGAGTGTCTGAAACTTCAGCTTCTTGCACAGGGCAA
TTACAAAGGAATTATAAAAGACCTTGTTGAAAATCATTTAGAAGATATTGCAGAGGGCAGGTATTCTTACTTAGCAAAGC
TTTACAATGTGTCTGCAAAAGAGGTGCAGAAGGCGGTAGATGCGATAAAAAAGTTAAATCCCAAACCGGGAAGCGTTTTT
GCACCGGAAGCAGAAGGGTATATAGTTCCTGATGTAGAAGTGATAAAAAGGGATGGGGAGTATTTGGTGTTAGTAAATGA
TTCGAATATTCCGAGGTTACGGATTAACAGTTACTATTATTCCATATTAGAAAGTTCTGATGAAGAAGCTAAAAAATATA
TAACCTCTAAACTCCAGTCAGCTATGTGGCTTATAAAAAGCATAGAGAGCAGAAAGGAAACGCTGTATAAAGTTGTAAAG
ACCATAGTTGACCTTCAGAAAGAGTTTTTGGATAAAGGGATAAATTACTTGAAGCCTTTGACGCAAAAGCAGGTTGCGGA
TATACTCGGAATACATGAGTCAACTGTCAGCAGAGCTATAAACGGAAAATACGTCCAGACTCCAAGAGGGACTTTTGAGC
TCAAGTTTTTCTTCCAGTCGGGGCTTTCCAGCAAGAATGGAAAAGAGTTTTCAGCTGAAACTGTTAAAAATTTGATTAAA
AAGTTGATAGAGGAGGAAGACCCTGCGAACCCCTTAAGCGACCAGAAAATAGCAGAGATTTTAAGGGAGAAAAATATAAA
TATTTCTAGGCGAACTGTGGCAAAATATAGGGAGGAATGCAATATCCCTTCAACTCTTAAGCGAAAAAGATATTAA

Upstream 100 bases:

>100_bases
TTATTTAAACGTTTAAATGTGATATAATATTTTAAAAAGCTTCATCTATTTAGTGGATTAAATTACCTTGTGTATAAGAT
GGGATTGAGGGTGCATGGGT

Downstream 100 bases:

>100_bases
GTTTTTGTATTGAAAAAGGTTTGAAATTAGTATAAAATAGTTGGTGTAGGTTATTTTTATAAAAGGTGGGACATTTAAAG
AATAACGGGACGGGAAGTGT

Product: DNA-directed RNA polymerase specialized sigma subunits, sigma54 homologs

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRMEFDLKLQQTQKLIMTPELKQAIEILQLNALELNELIQQELENNPLLEREEVEEDIYVVDVKELAKSIREMEKRMYYR
DSDDEEMDEFNYENFVSAKPGLSDHLLFQLHITPVPTRIRKICEYIIFSLTPSGYLKEDVREIAQMLECTEEEVLEALAV
VQSFDPPGVGARNLQECLKLQLLAQGNYKGIIKDLVENHLEDIAEGRYSYLAKLYNVSAKEVQKAVDAIKKLNPKPGSVF
APEAEGYIVPDVEVIKRDGEYLVLVNDSNIPRLRINSYYYSILESSDEEAKKYITSKLQSAMWLIKSIESRKETLYKVVK
TIVDLQKEFLDKGINYLKPLTQKQVADILGIHESTVSRAINGKYVQTPRGTFELKFFFQSGLSSKNGKEFSAETVKNLIK
KLIEEEDPANPLSDQKIAEILREKNINISRRTVAKYREECNIPSTLKRKRY

Sequences:

>Translated_451_residues
MRMEFDLKLQQTQKLIMTPELKQAIEILQLNALELNELIQQELENNPLLEREEVEEDIYVVDVKELAKSIREMEKRMYYR
DSDDEEMDEFNYENFVSAKPGLSDHLLFQLHITPVPTRIRKICEYIIFSLTPSGYLKEDVREIAQMLECTEEEVLEALAV
VQSFDPPGVGARNLQECLKLQLLAQGNYKGIIKDLVENHLEDIAEGRYSYLAKLYNVSAKEVQKAVDAIKKLNPKPGSVF
APEAEGYIVPDVEVIKRDGEYLVLVNDSNIPRLRINSYYYSILESSDEEAKKYITSKLQSAMWLIKSIESRKETLYKVVK
TIVDLQKEFLDKGINYLKPLTQKQVADILGIHESTVSRAINGKYVQTPRGTFELKFFFQSGLSSKNGKEFSAETVKNLIK
KLIEEEDPANPLSDQKIAEILREKNINISRRTVAKYREECNIPSTLKRKRY
>Mature_451_residues
MRMEFDLKLQQTQKLIMTPELKQAIEILQLNALELNELIQQELENNPLLEREEVEEDIYVVDVKELAKSIREMEKRMYYR
DSDDEEMDEFNYENFVSAKPGLSDHLLFQLHITPVPTRIRKICEYIIFSLTPSGYLKEDVREIAQMLECTEEEVLEALAV
VQSFDPPGVGARNLQECLKLQLLAQGNYKGIIKDLVENHLEDIAEGRYSYLAKLYNVSAKEVQKAVDAIKKLNPKPGSVF
APEAEGYIVPDVEVIKRDGEYLVLVNDSNIPRLRINSYYYSILESSDEEAKKYITSKLQSAMWLIKSIESRKETLYKVVK
TIVDLQKEFLDKGINYLKPLTQKQVADILGIHESTVSRAINGKYVQTPRGTFELKFFFQSGLSSKNGKEFSAETVKNLIK
KLIEEEDPANPLSDQKIAEILREKNINISRRTVAKYREECNIPSTLKRKRY

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=475, Percent_Identity=36, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 52093; Mature: 52093

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMEFDLKLQQTQKLIMTPELKQAIEILQLNALELNELIQQELENNPLLEREEVEEDIYV
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEE
VDVKELAKSIREMEKRMYYRDSDDEEMDEFNYENFVSAKPGLSDHLLFQLHITPVPTRIR
EEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHCCCCCCCCCCEEEEEEECCCCHHHH
KICEYIIFSLTPSGYLKEDVREIAQMLECTEEEVLEALAVVQSFDPPGVGARNLQECLKL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QLLAQGNYKGIIKDLVENHLEDIAEGRYSYLAKLYNVSAKEVQKAVDAIKKLNPKPGSVF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEE
APEAEGYIVPDVEVIKRDGEYLVLVNDSNIPRLRINSYYYSILESSDEEAKKYITSKLQS
CCCCCCEECCCHHHHHCCCCEEEEECCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHH
AMWLIKSIESRKETLYKVVKTIVDLQKEFLDKGINYLKPLTQKQVADILGIHESTVSRAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NGKYVQTPRGTFELKFFFQSGLSSKNGKEFSAETVKNLIKKLIEEEDPANPLSDQKIAEI
CCCEEECCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LREKNINISRRTVAKYREECNIPSTLKRKRY
HHHCCCCCHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MRMEFDLKLQQTQKLIMTPELKQAIEILQLNALELNELIQQELENNPLLEREEVEEDIYV
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEE
VDVKELAKSIREMEKRMYYRDSDDEEMDEFNYENFVSAKPGLSDHLLFQLHITPVPTRIR
EEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHCCCCCCCCCCEEEEEEECCCCHHHH
KICEYIIFSLTPSGYLKEDVREIAQMLECTEEEVLEALAVVQSFDPPGVGARNLQECLKL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QLLAQGNYKGIIKDLVENHLEDIAEGRYSYLAKLYNVSAKEVQKAVDAIKKLNPKPGSVF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEE
APEAEGYIVPDVEVIKRDGEYLVLVNDSNIPRLRINSYYYSILESSDEEAKKYITSKLQS
CCCCCCEECCCHHHHHCCCCEEEEECCCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHH
AMWLIKSIESRKETLYKVVKTIVDLQKEFLDKGINYLKPLTQKQVADILGIHESTVSRAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NGKYVQTPRGTFELKFFFQSGLSSKNGKEFSAETVKNLIKKLIEEEDPANPLSDQKIAEI
CCCEEECCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LREKNINISRRTVAKYREECNIPSTLKRKRY
HHHCCCCCHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]