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The map label for this gene is eno [H]

Identifier: 20808178

GI number: 20808178

Start: 1706007

End: 1707296

Strand: Reverse

Name: eno [H]

Synonym: TTE1759

Alternate gene names: 20808178

Gene position: 1707296-1706007 (Counterclockwise)

Preceding gene: 20808179

Following gene: 20808177

Centisome position: 63.48

GC content: 41.86

Gene sequence:

>1290_bases
ATGTCATCAATTATTGATATTTATGCGAGGGAAATATTAGATTCTCGTGGCAATCCTACGATTGAGGTAGAAGTTGAATT
AGACAGTGGTGCAGTGGGAAGAGCTGCTGTACCGTCTGGGGCTTCTACTGGGGCTTTTGAAGCAATTGAGTTAAGAGATG
GAGATAAGTCAAGATACTTAGGAAAAGGCGTATTGAAAGCAGTACAGAATGTAAATGACATTATTGCGCCAGAATTAATT
GGTATGGAAGCACAGGACCAGGTAGCAATTGATAAAGCTATGATTGAACTGGATGGAACGCCCAATAAAAGCAAGTTAGG
AGCAAATGCTATTTTAGGTGTGTCTTTGGCAGTTGCAAAAGCGGCAGCAGAAGAGCTGGGTCTTCCTCTGTACCAGTATT
TAGGGGGCGTAAATGCTAAGACACTTCCTGTTCCAATGATGAACATACTAAATGGCGGAAAGCATGCTGACAACAATGTA
GATATACAAGAGTTTATGATTATGCCTGTAGGAGCTCCCAATTTTAAAGAGGCTTTAAGGATGTGTTCAGAAGTTTATCA
CAGCCTGAAAAATGTGCTTCACTCCAAGGGATTGAGCACTACAGTTGGCGATGAGGGAGGTTTTGCGCCTAACCTTACAT
CGAATGAAGAGGCAATTAAAGTAATACTTGAGGCTATAGAAAAAGCCGGATATGTACCTGGGGAAGACATAGTTTTAGCG
TTGGACCCTGCGGCCACTGAAATGTACAAAGAAGATGGGAAATACCATTTTGAAGGAGAGGGAATTGTGAGAACTTCTGA
GGAAATGATAGAGTTCTGGGAGCAGCTCGTCAGCAAATACCCCATTGTTTCTATAGAAGATGGACTTGCAGAGGAAGACT
GGAACGGATGGAAACTCTTAACTGAAAGATTGGGCAAAAAGGTGCAGCTAGTAGGAGACGACCTATTTGTGACGAATACG
GAAAGGCTCTCAAAAGGCATTAACATGGGAGTTGCAAATTCAATACTCATAAAGCTCAATCAGATAGGTACTCTGACAGA
GACATTGGATGCCATTGAAATGGCTAAAAGGGCAGGCTACACTGCTATCGTATCTCACAGGTCAGGAGAGACGGAAGATA
CAACTATTGCTGATTTAGTAGTAGCTACAAATGTAGGTCAGATCAAAACAGGTGCTCCAGCAAGGACCGATAGAGTTGCA
AAATATAACCAATTATTAAGAATAGAAGAAGCATTGGGAAGCGTGGCCCAGTATCCTGGAAAAAATGCTTTCTACAACAT
CAGAAAATAA

Upstream 100 bases:

>100_bases
AGCGATATAGACGGAGCTTTGGTTGGCGGTGCCAGCCTTGTGGCTGAGGATTTTGCAAAGATTGTGAATTACTAAATTTT
ACATAGAAAGGGGAAGTGGT

Downstream 100 bases:

>100_bases
AAAAAGCTTAAAGGGGACTTTTCGCGTCCCCTTTTCTAAATTTCCTCTTGATTTGTAAAAGATGTACTTGTATAATATAT
ACTGTGGCATTATTGGGCTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSIIDIYAREILDSRGNPTIEVEVELDSGAVGRAAVPSGASTGAFEAIELRDGDKSRYLGKGVLKAVQNVNDIIAPELI
GMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAKAAAEELGLPLYQYLGGVNAKTLPVPMMNILNGGKHADNNV
DIQEFMIMPVGAPNFKEALRMCSEVYHSLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIKVILEAIEKAGYVPGEDIVLA
LDPAATEMYKEDGKYHFEGEGIVRTSEEMIEFWEQLVSKYPIVSIEDGLAEEDWNGWKLLTERLGKKVQLVGDDLFVTNT
ERLSKGINMGVANSILIKLNQIGTLTETLDAIEMAKRAGYTAIVSHRSGETEDTTIADLVVATNVGQIKTGAPARTDRVA
KYNQLLRIEEALGSVAQYPGKNAFYNIRK

Sequences:

>Translated_429_residues
MSSIIDIYAREILDSRGNPTIEVEVELDSGAVGRAAVPSGASTGAFEAIELRDGDKSRYLGKGVLKAVQNVNDIIAPELI
GMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAKAAAEELGLPLYQYLGGVNAKTLPVPMMNILNGGKHADNNV
DIQEFMIMPVGAPNFKEALRMCSEVYHSLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIKVILEAIEKAGYVPGEDIVLA
LDPAATEMYKEDGKYHFEGEGIVRTSEEMIEFWEQLVSKYPIVSIEDGLAEEDWNGWKLLTERLGKKVQLVGDDLFVTNT
ERLSKGINMGVANSILIKLNQIGTLTETLDAIEMAKRAGYTAIVSHRSGETEDTTIADLVVATNVGQIKTGAPARTDRVA
KYNQLLRIEEALGSVAQYPGKNAFYNIRK
>Mature_428_residues
SSIIDIYAREILDSRGNPTIEVEVELDSGAVGRAAVPSGASTGAFEAIELRDGDKSRYLGKGVLKAVQNVNDIIAPELIG
MEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAKAAAEELGLPLYQYLGGVNAKTLPVPMMNILNGGKHADNNVD
IQEFMIMPVGAPNFKEALRMCSEVYHSLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIKVILEAIEKAGYVPGEDIVLAL
DPAATEMYKEDGKYHFEGEGIVRTSEEMIEFWEQLVSKYPIVSIEDGLAEEDWNGWKLLTERLGKKVQLVGDDLFVTNTE
RLSKGINMGVANSILIKLNQIGTLTETLDAIEMAKRAGYTAIVSHRSGETEDTTIADLVVATNVGQIKTGAPARTDRVAK
YNQLLRIEEALGSVAQYPGKNAFYNIRK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=53.0092592592593, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=53.7037037037037, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI301897477, Length=427, Percent_Identity=53.3957845433255, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=427, Percent_Identity=53.3957845433255, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=48, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=24.702380952381, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=24.702380952381, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=24.702380952381, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=66.1971830985916, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.2407407407407, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.2407407407407, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=196, Percent_Identity=46.9387755102041, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=438, Percent_Identity=51.1415525114155, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.8845265588915, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.8845265588915, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.4226327944573, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=438, Percent_Identity=50, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=52.4249422632794, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=52.4249422632794, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=52.4249422632794, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=52.4249422632794, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=52.4249422632794, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=52.4249422632794, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46297; Mature: 46166

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIIDIYAREILDSRGNPTIEVEVELDSGAVGRAAVPSGASTGAFEAIELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
GKGVLKAVQNVNDIIAPELIGMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAK
HHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHHHHH
AAAEELGLPLYQYLGGVNAKTLPVPMMNILNGGKHADNNVDIQEFMIMPVGAPNFKEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MCSEVYHSLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIKVILEAIEKAGYVPGEDIVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDPAATEMYKEDGKYHFEGEGIVRTSEEMIEFWEQLVSKYPIVSIEDGLAEEDWNGWKLL
ECCHHHHHHHHCCCEEECCCCEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
TERLGKKVQLVGDDLFVTNTERLSKGINMGVANSILIKLNQIGTLTETLDAIEMAKRAGY
HHHCCCEEEEECCCEEEECHHHHHCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHCCC
TAIVSHRSGETEDTTIADLVVATNVGQIKTGAPARTDRVAKYNQLLRIEEALGSVAQYPG
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KNAFYNIRK
CCCCEECCC
>Mature Secondary Structure 
SSIIDIYAREILDSRGNPTIEVEVELDSGAVGRAAVPSGASTGAFEAIELRDGDKSRYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
GKGVLKAVQNVNDIIAPELIGMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAK
HHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHHHHH
AAAEELGLPLYQYLGGVNAKTLPVPMMNILNGGKHADNNVDIQEFMIMPVGAPNFKEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MCSEVYHSLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIKVILEAIEKAGYVPGEDIVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDPAATEMYKEDGKYHFEGEGIVRTSEEMIEFWEQLVSKYPIVSIEDGLAEEDWNGWKLL
ECCHHHHHHHHCCCEEECCCCEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
TERLGKKVQLVGDDLFVTNTERLSKGINMGVANSILIKLNQIGTLTETLDAIEMAKRAGY
HHHCCCEEEEECCCEEEECHHHHHCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHCCC
TAIVSHRSGETEDTTIADLVVATNVGQIKTGAPARTDRVAKYNQLLRIEEALGSVAQYPG
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KNAFYNIRK
CCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA