Click here to switch to the map view.

The map label for this gene is Sir2.2

Identifier: 20808159

GI number: 20808159

Start: 1685915

End: 1686667

Strand: Reverse

Name: Sir2.2

Synonym: TTE1740

Alternate gene names: 20808159

Gene position: 1686667-1685915 (Counterclockwise)

Preceding gene: 20808160

Following gene: 20808158

Centisome position: 62.71

GC content: 40.24

Gene sequence:

>753_bases
TTGTTTTTTATGGACAGCAAGAATTTGTTTAAAAAAGCGGCGGAACTTATAAAATCCTCTCAAAAGACTATGGTCTTAAC
AGGTGCAGGAATTTCTACGGAAAGCGGAATTCCTGATTTTAGAAGTCCTGGTACAGGCTTATGGGAAAATATGGACCCTA
CAGAAGTTTTGTCCACAAAAGTTTTGTTCAATTCACCTGAGGAGTTTTACAGGGTGGGCTTTAAAATTTTGTCCTCTATG
AGAAATGCAGAGCCAAATGAAGCTCATTATATATTGAGTGAAATGGAGAAGGAAGGAATAATTGCGGGGGTAATTACGCA
GAATATAGATAACCTTCATCAAAAGGCGGGCTCAAAAAAAGTTTACGAAGTTCATGGAAATACGAGAGAAGGAAGTTGTT
TAAGATGCGGGGAGAAGGTGAGTTTTGAACTTTTGGAAGAAAAAGTGGCAAAAGAAGAGATACCTCCCCGTTGCGACCGC
TGTGGAGGAATGCTAAGACCAGATGTGGTTCTTTTTGGGGACCCAATGCCTCATGCCTTTGATTTAGCTCTGAAAGAGGT
GCAGGAAAGTGATCTTTTGATAGTCATAGGTTCTTCGCTGGTCGTCGCCCCTGTGAATTTTTTACCTGGGATGGTGGACG
GCTTAATAATAATAAATGCTACAGAGACCCCTTATGACTATAAAGCTGACGTGGTGATAAGGGAGAAAGCTAGCTACGCA
CTTAGAAATATATGGAATTTAATAAAAAGTTGA

Upstream 100 bases:

>100_bases
TCAAGAAACAGGCAAAATAGGAGTTTTAGTGAAGAGAAAAATTAAAGAAAAAATGAAAAAGAACAATAAGATGTGATATA
CTGATACTTGAAAGGGGGGA

Downstream 100 bases:

>100_bases
ACATAGTGTAAAATTATCGTAGGATTTTTAAAGTGAAAAAACAAGAGACAACCCCTGTGATAAAATAGATTGTGGAAAAC
AAAAAACACGAAAGGGGTGT

Product: Sir2 family NAD-dependent protein deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MFFMDSKNLFKKAAELIKSSQKTMVLTGAGISTESGIPDFRSPGTGLWENMDPTEVLSTKVLFNSPEEFYRVGFKILSSM
RNAEPNEAHYILSEMEKEGIIAGVITQNIDNLHQKAGSKKVYEVHGNTREGSCLRCGEKVSFELLEEKVAKEEIPPRCDR
CGGMLRPDVVLFGDPMPHAFDLALKEVQESDLLIVIGSSLVVAPVNFLPGMVDGLIIINATETPYDYKADVVIREKASYA
LRNIWNLIKS

Sequences:

>Translated_250_residues
MFFMDSKNLFKKAAELIKSSQKTMVLTGAGISTESGIPDFRSPGTGLWENMDPTEVLSTKVLFNSPEEFYRVGFKILSSM
RNAEPNEAHYILSEMEKEGIIAGVITQNIDNLHQKAGSKKVYEVHGNTREGSCLRCGEKVSFELLEEKVAKEEIPPRCDR
CGGMLRPDVVLFGDPMPHAFDLALKEVQESDLLIVIGSSLVVAPVNFLPGMVDGLIIINATETPYDYKADVVIREKASYA
LRNIWNLIKS
>Mature_250_residues
MFFMDSKNLFKKAAELIKSSQKTMVLTGAGISTESGIPDFRSPGTGLWENMDPTEVLSTKVLFNSPEEFYRVGFKILSSM
RNAEPNEAHYILSEMEKEGIIAGVITQNIDNLHQKAGSKKVYEVHGNTREGSCLRCGEKVSFELLEEKVAKEEIPPRCDR
CGGMLRPDVVLFGDPMPHAFDLALKEVQESDLLIVIGSSLVVAPVNFLPGMVDGLIIINATETPYDYKADVVIREKASYA
LRNIWNLIKS

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI6912662, Length=221, Percent_Identity=36.1990950226244, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI6912660, Length=205, Percent_Identity=35.609756097561, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI63054862, Length=187, Percent_Identity=35.8288770053476, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI7657575, Length=252, Percent_Identity=32.9365079365079, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI13775602, Length=199, Percent_Identity=31.6582914572864, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI13787215, Length=227, Percent_Identity=34.3612334801762, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI6912664, Length=227, Percent_Identity=34.3612334801762, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI13775600, Length=199, Percent_Identity=31.6582914572864, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI300795542, Length=211, Percent_Identity=34.1232227488152, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI300797705, Length=191, Percent_Identity=31.413612565445, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI215982798, Length=186, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI300797577, Length=236, Percent_Identity=29.2372881355932, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI7706712, Length=259, Percent_Identity=26.2548262548263, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI300797597, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI308199517, Length=190, Percent_Identity=34.7368421052632, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17541892, Length=249, Percent_Identity=33.7349397590361, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71990482, Length=228, Percent_Identity=31.140350877193, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17567771, Length=274, Percent_Identity=28.1021897810219, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71990487, Length=230, Percent_Identity=30.8695652173913, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6325242, Length=248, Percent_Identity=32.258064516129, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324504, Length=243, Percent_Identity=30.4526748971193, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320163, Length=248, Percent_Identity=29.8387096774194, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648389, Length=193, Percent_Identity=32.6424870466321, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137536, Length=244, Percent_Identity=30.7377049180328, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28571445, Length=195, Percent_Identity=33.8461538461538, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24645650, Length=254, Percent_Identity=28.3464566929134, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28571443, Length=121, Percent_Identity=33.8842975206612, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571441, Length=121, Percent_Identity=33.8842975206612, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24650933, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD2_THETN (Q8R984)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623330.1
- ProteinModelPortal:   Q8R984
- SMR:   Q8R984
- GeneID:   996751
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1740
- NMPDR:   fig|273068.3.peg.1714
- HOGENOM:   HBG641281
- OMA:   HILEPAR
- ProtClustDB:   CLSK901388
- BioCyc:   TTEN273068:TTE1740-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27776; Mature: 27776

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFMDSKNLFKKAAELIKSSQKTMVLTGAGISTESGIPDFRSPGTGLWENMDPTEVLSTK
CCEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VLFNSPEEFYRVGFKILSSMRNAEPNEAHYILSEMEKEGIIAGVITQNIDNLHQKAGSKK
HHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCE
VYEVHGNTREGSCLRCGEKVSFELLEEKVAKEEIPPRCDRCGGMLRPDVVLFGDPMPHAF
EEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEECCCCCHHH
DLALKEVQESDLLIVIGSSLVVAPVNFLPGMVDGLIIINATETPYDYKADVVIREKASYA
HHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECHHHHH
LRNIWNLIKS
HHHHHHHHCC
>Mature Secondary Structure
MFFMDSKNLFKKAAELIKSSQKTMVLTGAGISTESGIPDFRSPGTGLWENMDPTEVLSTK
CCEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VLFNSPEEFYRVGFKILSSMRNAEPNEAHYILSEMEKEGIIAGVITQNIDNLHQKAGSKK
HHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCE
VYEVHGNTREGSCLRCGEKVSFELLEEKVAKEEIPPRCDRCGGMLRPDVVLFGDPMPHAF
EEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEECCCCCHHH
DLALKEVQESDLLIVIGSSLVVAPVNFLPGMVDGLIIINATETPYDYKADVVIREKASYA
HHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECHHHHH
LRNIWNLIKS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336