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The map label for this gene is hycI [H]

Identifier: 20808122

GI number: 20808122

Start: 1645486

End: 1645941

Strand: Reverse

Name: hycI [H]

Synonym: TTE1696

Alternate gene names: 20808122

Gene position: 1645941-1645486 (Counterclockwise)

Preceding gene: 20808123

Following gene: 20808121

Centisome position: 61.2

GC content: 41.23

Gene sequence:

>456_bases
GTGAAGAGAATAGTGATTACTGTAGGCAATGAGATGATGGGGGATGATGGAGCTGGCCCATTATTGGCCCGGCTTTTAGA
ACAAAACCCTATACCTGGATGGCAGGTCATTGATGGTGGGTCGGTGCCTGAGAATTACTTGCATAAAATACGGTATCTTA
AACCAGATGTGGTAGTAGTAGTCGACGCATGTGAAATGAATTTAAAAGCTGGTAGTATACGACTAATTTCAGAAAGCCAC
ATTGTTTGCAAGTTCATCCTGTCCACGCATAATTTACCTTTGACCTTTTTTATTTCCGCTTTGAGAGAAGTAGTGCCAGA
AGTTTATTTTATTGGTATTCAGCCTGCAGCAGTAGGTTTTGGCATGCCGATATCTTCTGAAGTATGTCGAGCTGTTCAGA
TTGTGTATCAAAAATTACAAACAGGAAACCTTGACTTTCCACACCTGGATGATTAG

Upstream 100 bases:

>100_bases
CCTAGTTTTTTACCAGAAGAAAAAGAATGGGCTAACTGTTTGCTTAAGCTTCTCCATTCTATACAAAAAGAACCAGCTAT
ATATGTGGTGGTGAGACTGA

Downstream 100 bases:

>100_bases
GGGGGTGATGCGTAGCCGAGTGAGACTGCAAGATAATAGGTTTTTAGGTTTTGTAAAATCTAAGAGTAAAAATTTATAAA
GGGGGAATTTTTATGCACAT

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKRIVITVGNEMMGDDGAGPLLARLLEQNPIPGWQVIDGGSVPENYLHKIRYLKPDVVVVVDACEMNLKAGSIRLISESH
IVCKFILSTHNLPLTFFISALREVVPEVYFIGIQPAAVGFGMPISSEVCRAVQIVYQKLQTGNLDFPHLDD

Sequences:

>Translated_151_residues
MKRIVITVGNEMMGDDGAGPLLARLLEQNPIPGWQVIDGGSVPENYLHKIRYLKPDVVVVVDACEMNLKAGSIRLISESH
IVCKFILSTHNLPLTFFISALREVVPEVYFIGIQPAAVGFGMPISSEVCRAVQIVYQKLQTGNLDFPHLDD
>Mature_151_residues
MKRIVITVGNEMMGDDGAGPLLARLLEQNPIPGWQVIDGGSVPENYLHKIRYLKPDVVVVVDACEMNLKAGSIRLISESH
IVCKFILSTHNLPLTFFISALREVVPEVYFIGIQPAAVGFGMPISSEVCRAVQIVYQKLQTGNLDFPHLDD

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=151, Percent_Identity=47.0198675496689, Blast_Score=140, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 16660; Mature: 16660

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIVITVGNEMMGDDGAGPLLARLLEQNPIPGWQVIDGGSVPENYLHKIRYLKPDVVVV
CCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEE
VDACEMNLKAGSIRLISESHIVCKFILSTHNLPLTFFISALREVVPEVYFIGIQPAAVGF
EECCCCCCCCCEEEEEECCCEEEHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCHHHHCC
GMPISSEVCRAVQIVYQKLQTGNLDFPHLDD
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKRIVITVGNEMMGDDGAGPLLARLLEQNPIPGWQVIDGGSVPENYLHKIRYLKPDVVVV
CCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEE
VDACEMNLKAGSIRLISESHIVCKFILSTHNLPLTFFISALREVVPEVYFIGIQPAAVGF
EECCCCCCCCCEEEEEECCCEEEHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCHHHHCC
GMPISSEVCRAVQIVYQKLQTGNLDFPHLDD
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]