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The map label for this gene is mraW

Identifier: 20808080

GI number: 20808080

Start: 1604596

End: 1605522

Strand: Reverse

Name: mraW

Synonym: TTE1653

Alternate gene names: 20808080

Gene position: 1605522-1604596 (Counterclockwise)

Preceding gene: 20808081

Following gene: 20808079

Centisome position: 59.7

GC content: 39.16

Gene sequence:

>927_bases
ATGGAATATGTTCATAAAAGCGTACTTCTCAAAGAAACGATAGAGCACTTAAATATAAATCCAGAAGGTATTTATGTGGA
TGGAACTTTAGGCGGTGGAGGTCATTCAGAGGAAATATTAAAGAGGCTTACTACTGGGAAATTAATTGCAATTGACAGAG
ATGAAGAAGCGATTTTAGCTGCCAAAGAAAGGCTAAAAGATTACAAAAATGTGATTTACATTAAAGACAATTTTAAGAAT
ATAAAAGAGATATTGAGAAATTTGGGGATTGAAAAAGTTGATGGAATTCTTCTGGATTTGGGGGTCTCCTCTTACCAGCT
GGAAGAAGTGGAGAGGGGCTTTTCTTATATGAAGGATGCTCCTCTTGATATGAGGATGGATAAAACTTCTCCTTTTTCCG
CCTATGATGTTGTGAACAGATATTCAGAAAAAGAGCTAGAGAGAGTGATAAGGGAATACGGAGAAGAGAAATGGGCTTCC
CGTATAGCGAAGTTTATAGTGGAGGAGAGAAAGAAGGGAGATATAAAGACTACTTCTCAGCTGGTGGAGATTATAAAGAA
AGCTATTCCGGCTTCTGCACGAAGAACAGGGCCTCATCCAGCGAAAAGAACTTTTCAAGCCATAAGGATAGAAGTGAATG
AGGAGCTTAAAGATTTAGACAGAGCTTTGGAAGATATGGTGGAGGTATTGAGGGGAAAAGGGAGGATAGCAGTGATTACT
TTCCACTCCTTGGAAGACAGAATAGTAAAAAATACTTTCAAAAAGCTTGAAAATCCCTGCACATGTCCTCCTGGTTTGCC
GTGCACTTGTGGGAAACAGCCTGTTATAAAAATTATCACAAAAAAACCTATTCTGCCTTCCAAGGAAGAAGTAGAAGAAA
ATCCTAGGTCGAGGAGTGCAAAGCTTAGAGTGGCGGAGAAACTGTGA

Upstream 100 bases:

>100_bases
TAAAGAAGTTTGGGAGGAGTATTCCAAGAGCACAGATGTGTCCTTTGAAGAGATTGCAGAGCATTTGGATAATTTTAACA
TATAATTTGGGGTAGAGAGC

Downstream 100 bases:

>100_bases
GTTCTAAAAGAAATGGAGGTAGAATAAATTGATAGTAGCTCAAAAAGATTATAATTATCAATATCCCCCATATACCTCTT
ACGACAAAAAGCCTGACAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEYVHKSVLLKETIEHLNINPEGIYVDGTLGGGGHSEEILKRLTTGKLIAIDRDEEAILAAKERLKDYKNVIYIKDNFKN
IKEILRNLGIEKVDGILLDLGVSSYQLEEVERGFSYMKDAPLDMRMDKTSPFSAYDVVNRYSEKELERVIREYGEEKWAS
RIAKFIVEERKKGDIKTTSQLVEIIKKAIPASARRTGPHPAKRTFQAIRIEVNEELKDLDRALEDMVEVLRGKGRIAVIT
FHSLEDRIVKNTFKKLENPCTCPPGLPCTCGKQPVIKIITKKPILPSKEEVEENPRSRSAKLRVAEKL

Sequences:

>Translated_308_residues
MEYVHKSVLLKETIEHLNINPEGIYVDGTLGGGGHSEEILKRLTTGKLIAIDRDEEAILAAKERLKDYKNVIYIKDNFKN
IKEILRNLGIEKVDGILLDLGVSSYQLEEVERGFSYMKDAPLDMRMDKTSPFSAYDVVNRYSEKELERVIREYGEEKWAS
RIAKFIVEERKKGDIKTTSQLVEIIKKAIPASARRTGPHPAKRTFQAIRIEVNEELKDLDRALEDMVEVLRGKGRIAVIT
FHSLEDRIVKNTFKKLENPCTCPPGLPCTCGKQPVIKIITKKPILPSKEEVEENPRSRSAKLRVAEKL
>Mature_308_residues
MEYVHKSVLLKETIEHLNINPEGIYVDGTLGGGGHSEEILKRLTTGKLIAIDRDEEAILAAKERLKDYKNVIYIKDNFKN
IKEILRNLGIEKVDGILLDLGVSSYQLEEVERGFSYMKDAPLDMRMDKTSPFSAYDVVNRYSEKELERVIREYGEEKWAS
RIAKFIVEERKKGDIKTTSQLVEIIKKAIPASARRTGPHPAKRTFQAIRIEVNEELKDLDRALEDMVEVLRGKGRIAVIT
FHSLEDRIVKNTFKKLENPCTCPPGLPCTCGKQPVIKIITKKPILPSKEEVEENPRSRSAKLRVAEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=37.5366568914956, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=38.5416666666667, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=46.031746031746, Blast_Score=251, Evalue=4e-68,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=37.8048780487805, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=37.8048780487805, Blast_Score=186, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_THETN (Q8R9F9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623251.1
- ProteinModelPortal:   Q8R9F9
- GeneID:   996208
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1653
- NMPDR:   fig|273068.3.peg.1635
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   TTEN273068:TTE1653-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35129; Mature: 35129

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 78-78 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYVHKSVLLKETIEHLNINPEGIYVDGTLGGGGHSEEILKRLTTGKLIAIDRDEEAILA
CCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHH
AKERLKDYKNVIYIKDNFKNIKEILRNLGIEKVDGILLDLGVSSYQLEEVERGFSYMKDA
HHHHHHHHHCEEEEECCHHHHHHHHHHCCCHHHCCCCEECCCCHHHHHHHHHHHHHHHCC
PLDMRMDKTSPFSAYDVVNRYSEKELERVIREYGEEKWASRIAKFIVEERKKGDIKTTSQ
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LVEIIKKAIPASARRTGPHPAKRTFQAIRIEVNEELKDLDRALEDMVEVLRGKGRIAVIT
HHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FHSLEDRIVKNTFKKLENPCTCPPGLPCTCGKQPVIKIITKKPILPSKEEVEENPRSRSA
ECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCCCHHH
KLRVAEKL
HHHHHHCC
>Mature Secondary Structure
MEYVHKSVLLKETIEHLNINPEGIYVDGTLGGGGHSEEILKRLTTGKLIAIDRDEEAILA
CCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHH
AKERLKDYKNVIYIKDNFKNIKEILRNLGIEKVDGILLDLGVSSYQLEEVERGFSYMKDA
HHHHHHHHHCEEEEECCHHHHHHHHHHCCCHHHCCCCEECCCCHHHHHHHHHHHHHHHCC
PLDMRMDKTSPFSAYDVVNRYSEKELERVIREYGEEKWASRIAKFIVEERKKGDIKTTSQ
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LVEIIKKAIPASARRTGPHPAKRTFQAIRIEVNEELKDLDRALEDMVEVLRGKGRIAVIT
HHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FHSLEDRIVKNTFKKLENPCTCPPGLPCTCGKQPVIKIITKKPILPSKEEVEENPRSRSA
ECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCCCHHH
KLRVAEKL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336