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The map label for this gene is Pnp2 [C]

Identifier: 20808016

GI number: 20808016

Start: 1543465

End: 1544247

Strand: Reverse

Name: Pnp2 [C]

Synonym: TTE1587

Alternate gene names: 20808016

Gene position: 1544247-1543465 (Counterclockwise)

Preceding gene: 20808017

Following gene: 20808015

Centisome position: 57.42

GC content: 40.61

Gene sequence:

>783_bases
ATGGAGAAAGCTATTATAGGTGGTACCGGAGTGTATGACGTAGCCGATAAAGTAGTTCAAAAAAAGGTTGAAACGAAATA
CGGAACAGTAGAGGTGGACGTGGTTACAGTAGAAGGAGAAGATATAGTTTTTTTAGCACGCCATGGGAAAGAGCATGGTG
TTCCCCCGCATTTGGTGAACTATAGAGCGAATATAATGGCTTTAAAGCAGCTTGGAGTGAAGTACATATATGCTACGGCT
GCTGTCGGTTCTTTAAACGAAAATTACCCTCCTGGAAGCGTAGTTATTCTAAAAGATTTTATAGATTTCACAAAATCCAG
GCCTTTGACTTTTTTTGAGGGAGAAGATGGGATAGTAAGGCATGTAGACATGAGTGATCCTTATTGTGTAAATTTGAGAG
GAAAATTTATAGAAGCGGCAAAAAAGGAGGGGCTTACTGTAAAAGGAGAAGCTGTATATGTATGTACGGAAGGGCCCAGA
TTTGAGACAGCTTCTGAGATAAAGGCTTATAAGATGCTGGGAGCTGATGTAGTAGGTATGACAAATGTTCCTGAGATAGT
CTTAGCTAAAGAATTGGGAATGTGCTATGCAGCCGTCGGTATAATCACAAACTGGGCTACAGGCCTTATAGCGGCAAGTT
CAGGTCCCGTGAGTCTTAGAGAGATTACAAGTGCAATAGAGATAAACAAGGAAAAGGTGATAAGAACCTTTATAAGGGTA
TTTACTGAAGAGACCTTAGACCAGGAGCACTGCAACTGTGCCAATTCAGTAATTGAATTATAA

Upstream 100 bases:

>100_bases
AAATTTGAAGTTAGGGATTATGCCTTTTTGGCAGTAGCTCTATTGATTTTGATTGTCGCAGTGTATTTTAAATTTAAATA
GCTGTAGGAGGTAAAGTCCT

Downstream 100 bases:

>100_bases
AATTACAGGGAAGAGGGGGGATTAACCCAAAGAGTATTCTTTTTATAATTCTCTAAAATTAAAATTTTGAAAAAGGGGGA
AATGGGTGTGAAGGAAGTTT

Product: purine nucleoside phosphorylase

Products: ribose-1-phosphate; xanthine [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEKAIIGGTGVYDVADKVVQKKVETKYGTVEVDVVTVEGEDIVFLARHGKEHGVPPHLVNYRANIMALKQLGVKYIYATA
AVGSLNENYPPGSVVILKDFIDFTKSRPLTFFEGEDGIVRHVDMSDPYCVNLRGKFIEAAKKEGLTVKGEAVYVCTEGPR
FETASEIKAYKMLGADVVGMTNVPEIVLAKELGMCYAAVGIITNWATGLIAASSGPVSLREITSAIEINKEKVIRTFIRV
FTEETLDQEHCNCANSVIEL

Sequences:

>Translated_260_residues
MEKAIIGGTGVYDVADKVVQKKVETKYGTVEVDVVTVEGEDIVFLARHGKEHGVPPHLVNYRANIMALKQLGVKYIYATA
AVGSLNENYPPGSVVILKDFIDFTKSRPLTFFEGEDGIVRHVDMSDPYCVNLRGKFIEAAKKEGLTVKGEAVYVCTEGPR
FETASEIKAYKMLGADVVGMTNVPEIVLAKELGMCYAAVGIITNWATGLIAASSGPVSLREITSAIEINKEKVIRTFIRV
FTEETLDQEHCNCANSVIEL
>Mature_260_residues
MEKAIIGGTGVYDVADKVVQKKVETKYGTVEVDVVTVEGEDIVFLARHGKEHGVPPHLVNYRANIMALKQLGVKYIYATA
AVGSLNENYPPGSVVILKDFIDFTKSRPLTFFEGEDGIVRHVDMSDPYCVNLRGKFIEAAKKEGLTVKGEAVYVCTEGPR
FETASEIKAYKMLGADVVGMTNVPEIVLAKELGMCYAAVGIITNWATGLIAASSGPVSLREITSAIEINKEKVIRTFIRV
FTEETLDQEHCNCANSVIEL

Specific function: The Nucleoside Phosphorylases Catalyze The Phosphorolytic Breakdown Of The N-Glycosidic Bond In The Nucleoside Molecule, With The Formation Of The Corresponding Free Bases And Pentose-1-Phosphate. This Protein Can Degrade All Purine Nucleosides Except Ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI47132622, Length=208, Percent_Identity=41.8269230769231, Blast_Score=171, Evalue=4e-43,
Organism=Homo sapiens, GI157168362, Length=219, Percent_Identity=31.0502283105023, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788746, Length=247, Percent_Identity=31.1740890688259, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71980569, Length=208, Percent_Identity=40.3846153846154, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17541190, Length=202, Percent_Identity=29.2079207920792, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323045, Length=221, Percent_Identity=31.6742081447964, Blast_Score=108, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323238, Length=175, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130079, Length=271, Percent_Identity=33.5793357933579, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI221459247, Length=171, Percent_Identity=36.2573099415205, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45552887, Length=217, Percent_Identity=30.4147465437788, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45552885, Length=218, Percent_Identity=30.2752293577982, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656093, Length=218, Percent_Identity=30.2752293577982, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656090, Length=218, Percent_Identity=30.2752293577982, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010044
- InterPro:   IPR000845
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 28409; Mature: 28409

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAIIGGTGVYDVADKVVQKKVETKYGTVEVDVVTVEGEDIVFLARHGKEHGVPPHLVN
CCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCHHHH
YRANIMALKQLGVKYIYATAAVGSLNENYPPGSVVILKDFIDFTKSRPLTFFEGEDGIVR
HHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCEEEEEHHHHHHHCCCCEEEEECCCCEEE
HVDMSDPYCVNLRGKFIEAAKKEGLTVKGEAVYVCTEGPRFETASEIKAYKMLGADVVGM
EEECCCCEEEECCHHHHHHHHHCCCEEECCEEEEECCCCCCCCHHHHHHHHHHCCCEECC
TNVPEIVLAKELGMCYAAVGIITNWATGLIAASSGPVSLREITSAIEINKEKVIRTFIRV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHH
FTEETLDQEHCNCANSVIEL
HHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEKAIIGGTGVYDVADKVVQKKVETKYGTVEVDVVTVEGEDIVFLARHGKEHGVPPHLVN
CCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCHHHH
YRANIMALKQLGVKYIYATAAVGSLNENYPPGSVVILKDFIDFTKSRPLTFFEGEDGIVR
HHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCEEEEEHHHHHHHCCCCEEEEECCCCEEE
HVDMSDPYCVNLRGKFIEAAKKEGLTVKGEAVYVCTEGPRFETASEIKAYKMLGADVVGM
EEECCCCEEEECCHHHHHHHHHCCCEEECCEEEEECCCCCCCCHHHHHHHHHHCCCEECC
TNVPEIVLAKELGMCYAAVGIITNWATGLIAASSGPVSLREITSAIEINKEKVIRTFIRV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHH
FTEETLDQEHCNCANSVIEL
HHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: xanthosine; phosphate [C]

Specific reaction: xanthosine + phosphate = ribose-1-phosphate + xanthine [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]