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The map label for this gene is trpA

Identifier: 20808006

GI number: 20808006

Start: 1534296

End: 1535084

Strand: Reverse

Name: trpA

Synonym: TTE1577

Alternate gene names: 20808006

Gene position: 1535084-1534296 (Counterclockwise)

Preceding gene: 20808007

Following gene: 20808005

Centisome position: 57.08

GC content: 39.92

Gene sequence:

>789_bases
GTGAATAGAATTGATAGAAGGTTTGATGACCTCAAAAAAGAAGGTAAAAAAGCCCTTATAACTTTCATTACTGCCGGAGA
CCCAGACATTGACACAACTTATGATATAGTTTTAGCCCTTGAAGAAGCAGGAGCGGATATAATTGAACTTGGTATACCCT
ATTCTGACCCCTTGGCCGATGGGCCGACAATCCAAGCTTCTTCCCAGAGAGCTTTGGCTAAGGGAATAAAAATTAAAGAT
ATAATGGATTTGGTAATGAGAATAAGAGAAAAGAGCGACATTCCTATTGTCTATCTTGTTTATTACAACTCTGTCTTCAA
ATACGGCATAGAGAAGTTTTTGGAGGATTCGAAAAACGCAGGAGTTGATGGACTCATTATTCCCGACCTCCCTTTGGAGG
AGAGGAAAGAAGTCTTGGATGTAGCTGATAAGTACGAAATTTATTTAATACCTCTGGTAGCTCCTACTTCAAAGGAGAGA
ATAAAGGGAATTACTGAAAACGGTAAGGGATTTGTATACTGCGTGACTCTGACTGGAGTTACGGGAGCGAGGGAGGAAAT
TACAACAGACCTTGAGGAATACATGAGGCTGGTATCTTCATATACCAGCATGCCAAAGGCTTTAGGGTTTGGCATATCAG
GACCCGAAATGGCAAGAAAGCTTAAAAGTTTGAGCGATGGAATTGTGGTAGGAAGCGCTATTGTAGAAAGAATTGCTAAG
GGGTATAATAGATGTGAAATGTTAAAAGAGGTAAAGGAATTTGTGTTAAGCTTAAGAGAAGTCTTGTGA

Upstream 100 bases:

>100_bases
TGGCTAAGAAATTGGATGAAGACAAGATAATTGTAGTAAATCTTTCTGGAAGAGGGGACAAAGATGTAAATACCGTCGCA
AAAGTAATGGGGGTGGAATT

Downstream 100 bases:

>100_bases
AAGGTGATAAGTATGGAATTTAAAATAGTGGGAAATAAAGATAAAAAGCCGAGTGTAAAAATAGGGGAGGTAACCGTTGG
AGAGGGGAAACTTATAATTG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNRIDRRFDDLKKEGKKALITFITAGDPDIDTTYDIVLALEEAGADIIELGIPYSDPLADGPTIQASSQRALAKGIKIKD
IMDLVMRIREKSDIPIVYLVYYNSVFKYGIEKFLEDSKNAGVDGLIIPDLPLEERKEVLDVADKYEIYLIPLVAPTSKER
IKGITENGKGFVYCVTLTGVTGAREEITTDLEEYMRLVSSYTSMPKALGFGISGPEMARKLKSLSDGIVVGSAIVERIAK
GYNRCEMLKEVKEFVLSLREVL

Sequences:

>Translated_262_residues
MNRIDRRFDDLKKEGKKALITFITAGDPDIDTTYDIVLALEEAGADIIELGIPYSDPLADGPTIQASSQRALAKGIKIKD
IMDLVMRIREKSDIPIVYLVYYNSVFKYGIEKFLEDSKNAGVDGLIIPDLPLEERKEVLDVADKYEIYLIPLVAPTSKER
IKGITENGKGFVYCVTLTGVTGAREEITTDLEEYMRLVSSYTSMPKALGFGISGPEMARKLKSLSDGIVVGSAIVERIAK
GYNRCEMLKEVKEFVLSLREVL
>Mature_262_residues
MNRIDRRFDDLKKEGKKALITFITAGDPDIDTTYDIVLALEEAGADIIELGIPYSDPLADGPTIQASSQRALAKGIKIKD
IMDLVMRIREKSDIPIVYLVYYNSVFKYGIEKFLEDSKNAGVDGLIIPDLPLEERKEVLDVADKYEIYLIPLVAPTSKER
IKGITENGKGFVYCVTLTGVTGAREEITTDLEEYMRLVSSYTSMPKALGFGISGPEMARKLKSLSDGIVVGSAIVERIAK
GYNRCEMLKEVKEFVLSLREVL

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=263, Percent_Identity=35.7414448669202, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321412, Length=236, Percent_Identity=41.5254237288136, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_THETN (Q8R9N0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623177.1
- ProteinModelPortal:   Q8R9N0
- SMR:   Q8R9N0
- GeneID:   995981
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1577
- NMPDR:   fig|273068.3.peg.1524
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   TTEN273068:TTE1577-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29155; Mature: 29155

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDRRFDDLKKEGKKALITFITAGDPDIDTTYDIVLALEEAGADIIELGIPYSDPLAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHEEEEHHHCCCEEEECCCCCCCCCC
GPTIQASSQRALAKGIKIKDIMDLVMRIREKSDIPIVYLVYYNSVFKYGIEKFLEDSKNA
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
GVDGLIIPDLPLEERKEVLDVADKYEIYLIPLVAPTSKERIKGITENGKGFVYCVTLTGV
CCCEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEEECCC
TGAREEITTDLEEYMRLVSSYTSMPKALGFGISGPEMARKLKSLSDGIVVGSAIVERIAK
CCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
GYNRCEMLKEVKEFVLSLREVL
HHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNRIDRRFDDLKKEGKKALITFITAGDPDIDTTYDIVLALEEAGADIIELGIPYSDPLAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHEEEEHHHCCCEEEECCCCCCCCCC
GPTIQASSQRALAKGIKIKDIMDLVMRIREKSDIPIVYLVYYNSVFKYGIEKFLEDSKNA
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
GVDGLIIPDLPLEERKEVLDVADKYEIYLIPLVAPTSKERIKGITENGKGFVYCVTLTGV
CCCEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEEECCC
TGAREEITTDLEEYMRLVSSYTSMPKALGFGISGPEMARKLKSLSDGIVVGSAIVERIAK
CCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
GYNRCEMLKEVKEFVLSLREVL
HHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336