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The map label for this gene is thiH [H]

Identifier: 20807997

GI number: 20807997

Start: 1527550

End: 1529055

Strand: Direct

Name: thiH [H]

Synonym: TTE1568

Alternate gene names: 20807997

Gene position: 1527550-1529055 (Clockwise)

Preceding gene: 20807990

Following gene: 20807998

Centisome position: 56.8

GC content: 38.98

Gene sequence:

>1506_bases
ATGGCTGTTGTGCTTAGTGAAAAAGAGAAAAAATGGATGGAAGGAGTAATCAAAGAGGAACAAATCACCAGATATCTTGA
AAACGGAAGAGACTTCATTGATGACGAAGAAATATGGGAAAAACTGAGGAAAAACCAGAATCCTGATAAGCAGAGGATAA
GAGAAATAATTCAAAAGTCACTAGAAATAGAAACTCTTGAACCTGATGAAACGGCTGCTCTTCTCAATGTTAAAGATCCT
GAACTATGGCAAGAAATTTTTGATGCAGCAGCAAAAATCAAGAAAAAAGTTTATGACAGCAGAATTGTAACCTTTGCGCC
CCTTTATTGCAGCAACCTGTGTGTAAATAATTGCGTCTACTGCGGATTTAGGAGAGAAAATACAGTCATCAAAAGAAGAA
GGTTAACATTAGAAGAAGTAAGAAGAGAAGCAGAAGTTCTAGCAGGAGAAATAGGCCATAAGCGTTTAATTGCAGTATAT
GGTGAGCATCCTACTACAGATGCTAAATATATTGCAGATACAATAAGAACTATATATGAAGTCAAAGTTAAAACAAAAAA
TGGATATGGGCAGATAAGAAGAGTAAATGTAAACGCCGCCCCCATGTCTATAGAAGAATTAGAAATGCTTCGGGATGTAG
GAATAGGAACCTATCAGGTTTTCCAAGAAACCTATCATCATGAGACCTATAAAAAGCTACACCCAGAAGGAACCATAAAA
TCCCATTATCAGTGGAGATTGTACTGTCACCACCGAGCACTGGAAGCAGGAGTTGACGATGTAGCTTTGGGAGTGCTCTT
TGGACTTTATGACTGGAGATTTGAAGTAATGGGTCTTTTATATCACGCAAGAAATCTAGAGAAATTTTTTGGCATAGGAC
CTCATACCATATCTTTCCCAAGGTTAGAACCTGCTGCTAATACACCTTTTGCTCAAGAATCAAAATACAAAGTATCTGAT
GAAGATTTCAAAAAGTTAATAGCTGTCATAAGGCTTTCTGTACCTTATACTGGTATGATTCTAACCGCCAGAGAACCTGC
TCACTTAAGGGATGAAATTCTCTCCATGGGCATAGTCACTCAAACGGATGCTTCTACAAAAATTGGAATAGGCGCCTACT
CTGACAGGTACACCGAGCAGGAAGCAGAGAGACAGCAGTTCCTGCTAGGGGATACCAGAAGCCTGGACGAATTGATAAGA
GAACTGGCAAAAATGGGGTATATAACAAGTTTCTGCACAGCAGGTTACAGATGTGGAAGAACAGGCAAACACATAATGAC
CCTTTTAAAAACAGGAAGAGAAGCTGTTTTCTGCAAGCTAAACGCCGTACTCACTTTTAGAGAATGGCTGGACGATTTTG
CAAGCGAGGAAACAAAAATAGCCGGTGAACAAGTAATACAGAAAGAAATTGAAGAAATCAAAGAAAGAGTTCCCGCAGAA
GTATTTTCAAAGCTCATGGAGTATTACAGGAGGATACAGAATGGAGAAAGAGACCTCTACTTCTAA

Upstream 100 bases:

>100_bases
ATCATTTTTGTTGAAAATTTAACAATCCTATTGTATAATGTAATTGAAATGCTGAGCCCAAGGAGGTACTCTGAGGGCAA
GGGAAGGAGGAAATTTAAAA

Downstream 100 bases:

>100_bases
GAAGCTATTGATCGAAGAATTTACCAGGCTCTCAGACTTAATAAAATCAAAGTACGGCGTGAGCTTGTGGCTGGTGGAAA
TTATGGGAAAAAGGCATTCG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MAVVLSEKEKKWMEGVIKEEQITRYLENGRDFIDDEEIWEKLRKNQNPDKQRIREIIQKSLEIETLEPDETAALLNVKDP
ELWQEIFDAAAKIKKKVYDSRIVTFAPLYCSNLCVNNCVYCGFRRENTVIKRRRLTLEEVRREAEVLAGEIGHKRLIAVY
GEHPTTDAKYIADTIRTIYEVKVKTKNGYGQIRRVNVNAAPMSIEELEMLRDVGIGTYQVFQETYHHETYKKLHPEGTIK
SHYQWRLYCHHRALEAGVDDVALGVLFGLYDWRFEVMGLLYHARNLEKFFGIGPHTISFPRLEPAANTPFAQESKYKVSD
EDFKKLIAVIRLSVPYTGMILTAREPAHLRDEILSMGIVTQTDASTKIGIGAYSDRYTEQEAERQQFLLGDTRSLDELIR
ELAKMGYITSFCTAGYRCGRTGKHIMTLLKTGREAVFCKLNAVLTFREWLDDFASEETKIAGEQVIQKEIEEIKERVPAE
VFSKLMEYYRRIQNGERDLYF

Sequences:

>Translated_501_residues
MAVVLSEKEKKWMEGVIKEEQITRYLENGRDFIDDEEIWEKLRKNQNPDKQRIREIIQKSLEIETLEPDETAALLNVKDP
ELWQEIFDAAAKIKKKVYDSRIVTFAPLYCSNLCVNNCVYCGFRRENTVIKRRRLTLEEVRREAEVLAGEIGHKRLIAVY
GEHPTTDAKYIADTIRTIYEVKVKTKNGYGQIRRVNVNAAPMSIEELEMLRDVGIGTYQVFQETYHHETYKKLHPEGTIK
SHYQWRLYCHHRALEAGVDDVALGVLFGLYDWRFEVMGLLYHARNLEKFFGIGPHTISFPRLEPAANTPFAQESKYKVSD
EDFKKLIAVIRLSVPYTGMILTAREPAHLRDEILSMGIVTQTDASTKIGIGAYSDRYTEQEAERQQFLLGDTRSLDELIR
ELAKMGYITSFCTAGYRCGRTGKHIMTLLKTGREAVFCKLNAVLTFREWLDDFASEETKIAGEQVIQKEIEEIKERVPAE
VFSKLMEYYRRIQNGERDLYF
>Mature_500_residues
AVVLSEKEKKWMEGVIKEEQITRYLENGRDFIDDEEIWEKLRKNQNPDKQRIREIIQKSLEIETLEPDETAALLNVKDPE
LWQEIFDAAAKIKKKVYDSRIVTFAPLYCSNLCVNNCVYCGFRRENTVIKRRRLTLEEVRREAEVLAGEIGHKRLIAVYG
EHPTTDAKYIADTIRTIYEVKVKTKNGYGQIRRVNVNAAPMSIEELEMLRDVGIGTYQVFQETYHHETYKKLHPEGTIKS
HYQWRLYCHHRALEAGVDDVALGVLFGLYDWRFEVMGLLYHARNLEKFFGIGPHTISFPRLEPAANTPFAQESKYKVSDE
DFKKLIAVIRLSVPYTGMILTAREPAHLRDEILSMGIVTQTDASTKIGIGAYSDRYTEQEAERQQFLLGDTRSLDELIRE
LAKMGYITSFCTAGYRCGRTGKHIMTLLKTGREAVFCKLNAVLTFREWLDDFASEETKIAGEQVIQKEIEEIKERVPAEV
FSKLMEYYRRIQNGERDLYF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=351, Percent_Identity=28.7749287749288, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 58218; Mature: 58087

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVLSEKEKKWMEGVIKEEQITRYLENGRDFIDDEEIWEKLRKNQNPDKQRIREIIQKS
CEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LEIETLEPDETAALLNVKDPELWQEIFDAAAKIKKKVYDSRIVTFAPLYCSNLCVNNCVY
CCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHEEHHHHHHHHHHHCCEE
CGFRRENTVIKRRRLTLEEVRREAEVLAGEIGHKRLIAVYGEHPTTDAKYIADTIRTIYE
ECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
VKVKTKNGYGQIRRVNVNAAPMSIEELEMLRDVGIGTYQVFQETYHHETYKKLHPEGTIK
EEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
SHYQWRLYCHHRALEAGVDDVALGVLFGLYDWRFEVMGLLYHARNLEKFFGIGPHTISFP
CCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RLEPAANTPFAQESKYKVSDEDFKKLIAVIRLSVPYTGMILTAREPAHLRDEILSMGIVT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCEE
QTDASTKIGIGAYSDRYTEQEAERQQFLLGDTRSLDELIRELAKMGYITSFCTAGYRCGR
ECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGKHIMTLLKTGREAVFCKLNAVLTFREWLDDFASEETKIAGEQVIQKEIEEIKERVPAE
CHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
VFSKLMEYYRRIQNGERDLYF
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AVVLSEKEKKWMEGVIKEEQITRYLENGRDFIDDEEIWEKLRKNQNPDKQRIREIIQKS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LEIETLEPDETAALLNVKDPELWQEIFDAAAKIKKKVYDSRIVTFAPLYCSNLCVNNCVY
CCEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHEEHHHHHHHHHHHCCEE
CGFRRENTVIKRRRLTLEEVRREAEVLAGEIGHKRLIAVYGEHPTTDAKYIADTIRTIYE
ECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
VKVKTKNGYGQIRRVNVNAAPMSIEELEMLRDVGIGTYQVFQETYHHETYKKLHPEGTIK
EEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
SHYQWRLYCHHRALEAGVDDVALGVLFGLYDWRFEVMGLLYHARNLEKFFGIGPHTISFP
CCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RLEPAANTPFAQESKYKVSDEDFKKLIAVIRLSVPYTGMILTAREPAHLRDEILSMGIVT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCEE
QTDASTKIGIGAYSDRYTEQEAERQQFLLGDTRSLDELIRELAKMGYITSFCTAGYRCGR
ECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGKHIMTLLKTGREAVFCKLNAVLTFREWLDDFASEETKIAGEQVIQKEIEEIKERVPAE
CHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
VFSKLMEYYRRIQNGERDLYF
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]