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The map label for this gene is ThiC

Identifier: 20807994

GI number: 20807994

Start: 1523327

End: 1524625

Strand: Reverse

Name: ThiC

Synonym: TTE1565

Alternate gene names: 20807994

Gene position: 1524625-1523327 (Counterclockwise)

Preceding gene: 20807995

Following gene: 20807993

Centisome position: 56.69

GC content: 39.18

Gene sequence:

>1299_bases
ATGACTCAATTAGAATATGCCTTATCAGGCATAATTACAAAAGAGATGAAAATAGTTGCGGACTATGAAGGTGTGTCTGA
AGAATTTATCCTAGAAGAAGTCAAAAAAGGTGAAATAGTAATACCAGCAAATGTTAACCACATAAATCTCGTTCCCAAGG
GCATAGGAAAAGGATTGTCAACAAAAGTAAATGCTAACATTGGAACTTCCGATGCCTTTCCTGAAATTGGAAAAGAAATT
GAAAAGTTGAAAGTAGCTATAGATGCTGGAGCAGATGCAGTAATGGATTTAAGCACTGGTGGCGATGTTAACAAGTCACG
ACGAGAAATAATCAAAAACTCATCTGTACCTGTTGGTACAGTGCCTATGTATCAAGCAGCAGTAGAATCTATATCAAGAT
ACGGCAGCATTGTAGCAATGCCTGAGGAATTTATTTTTAAAGTGATAGAAGAACAAGCAAAAGATGGAGTTGATTTCATC
ACTGTCCACTGCGGTCTTACTTTAGAATCCCTTAAAAGACTTAAAGACAACAAACGAATTATGAATGTAGTAAGTAGAGG
AGGAGCTTTTACAATAGCCTGGATGATTCATAATGAAAAAGAGAACCCCCTTTATCAATATTTTGATAGGCTTTTAGACA
TAGCAAAGAAATATGATGTAACGCTGAGTCTTGGGGATGGGCTTCGACCAGGATGTCTTGAAGATGCAACCGATGCAGCT
CAAATTCAAGAGCTGATAATTTTAGGCGAACTTGTTAAAAAGGCTAGAGAAGCAGGAGTTCAAGTAATGGTAGAAGGACC
TGGTCATGTTCCCATTGATCAAATCGAAGCCAATGTAAAACTACAGAAAAGTCTTTGTCACAATGCTCCTTTTTATGTGC
TAGGGCCGGTAGTAACAGATATCGCTCCGGGCTACGATCACATCACTGCTGCAATAGGAGGAGCGATTGCTGCTTTTGCA
GGAGCAGATTTTCTGTGTTATGTCACCCCTGCAGAACATCTTGGACTTCCTGACATACAAGATGTCAAAGAAGGTGTCAT
TGCTGCTAAAATTGCTGCCCATGCTGCAGATATAGCGAAAGGTATAAAAAAGGCCAGAGAAAAAGATTTAGCTATGGCAA
AAGCCAGGGCAAATTTAGACTGGAATGAACAAATACAACTATCCATTGACCCTGAAAAAGCCGAAAAATACCGTGTAACT
AAGAATAAGCCTAATGTGGAGACCTGCAGTATGTGTGGGAAATTCTGTGCCATGCAAATAGTGAGTGAATATTTAGGAAC
TCCTACTACAAGTTGCTAA

Upstream 100 bases:

>100_bases
ATGCCTGCGAAGGGAAGCACGTTTTACGAAATTTGTAAAAAGTGCTCACCTTTTCAGGTGGGCATATTTTATTATTAAAA
ACTTCAAGGAGGTTTTTATC

Downstream 100 bases:

>100_bases
GAGGCTATTTTTTAGTTCAAATTACTCATACAATCATGTTAAAATTAATCGCAGTGAGCAACAAAATGCTCACCTGGGTC
GCAGTAACCCCAGTTAACAA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTQLEYALSGIITKEMKIVADYEGVSEEFILEEVKKGEIVIPANVNHINLVPKGIGKGLSTKVNANIGTSDAFPEIGKEI
EKLKVAIDAGADAVMDLSTGGDVNKSRREIIKNSSVPVGTVPMYQAAVESISRYGSIVAMPEEFIFKVIEEQAKDGVDFI
TVHCGLTLESLKRLKDNKRIMNVVSRGGAFTIAWMIHNEKENPLYQYFDRLLDIAKKYDVTLSLGDGLRPGCLEDATDAA
QIQELIILGELVKKAREAGVQVMVEGPGHVPIDQIEANVKLQKSLCHNAPFYVLGPVVTDIAPGYDHITAAIGGAIAAFA
GADFLCYVTPAEHLGLPDIQDVKEGVIAAKIAAHAADIAKGIKKAREKDLAMAKARANLDWNEQIQLSIDPEKAEKYRVT
KNKPNVETCSMCGKFCAMQIVSEYLGTPTTSC

Sequences:

>Translated_432_residues
MTQLEYALSGIITKEMKIVADYEGVSEEFILEEVKKGEIVIPANVNHINLVPKGIGKGLSTKVNANIGTSDAFPEIGKEI
EKLKVAIDAGADAVMDLSTGGDVNKSRREIIKNSSVPVGTVPMYQAAVESISRYGSIVAMPEEFIFKVIEEQAKDGVDFI
TVHCGLTLESLKRLKDNKRIMNVVSRGGAFTIAWMIHNEKENPLYQYFDRLLDIAKKYDVTLSLGDGLRPGCLEDATDAA
QIQELIILGELVKKAREAGVQVMVEGPGHVPIDQIEANVKLQKSLCHNAPFYVLGPVVTDIAPGYDHITAAIGGAIAAFA
GADFLCYVTPAEHLGLPDIQDVKEGVIAAKIAAHAADIAKGIKKAREKDLAMAKARANLDWNEQIQLSIDPEKAEKYRVT
KNKPNVETCSMCGKFCAMQIVSEYLGTPTTSC
>Mature_431_residues
TQLEYALSGIITKEMKIVADYEGVSEEFILEEVKKGEIVIPANVNHINLVPKGIGKGLSTKVNANIGTSDAFPEIGKEIE
KLKVAIDAGADAVMDLSTGGDVNKSRREIIKNSSVPVGTVPMYQAAVESISRYGSIVAMPEEFIFKVIEEQAKDGVDFIT
VHCGLTLESLKRLKDNKRIMNVVSRGGAFTIAWMIHNEKENPLYQYFDRLLDIAKKYDVTLSLGDGLRPGCLEDATDAAQ
IQELIILGELVKKAREAGVQVMVEGPGHVPIDQIEANVKLQKSLCHNAPFYVLGPVVTDIAPGYDHITAAIGGAIAAFAG
ADFLCYVTPAEHLGLPDIQDVKEGVIAAKIAAHAADIAKGIKKAREKDLAMAKARANLDWNEQIQLSIDPEKAEKYRVTK
NKPNVETCSMCGKFCAMQIVSEYLGTPTTSC

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=464, Percent_Identity=45.2586206896552, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_THETN (Q8R9P1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623165.1
- GeneID:   995714
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1565
- NMPDR:   fig|273068.3.peg.1512
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   TTEN273068:TTE1565-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 46831; Mature: 46699

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEYALSGIITKEMKIVADYEGVSEEFILEEVKKGEIVIPANVNHINLVPKGIGKGLS
CCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCEEEECCCCEEEECCHHCCCCCC
TKVNANIGTSDAFPEIGKEIEKLKVAIDAGADAVMDLSTGGDVNKSRREIIKNSSVPVGT
EEECCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
VPMYQAAVESISRYGSIVAMPEEFIFKVIEEQAKDGVDFITVHCGLTLESLKRLKDNKRI
CHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHH
MNVVSRGGAFTIAWMIHNEKENPLYQYFDRLLDIAKKYDVTLSLGDGLRPGCLEDATDAA
HHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHH
QIQELIILGELVKKAREAGVQVMVEGPGHVPIDQIEANVKLQKSLCHNAPFYVLGPVVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCCEEECHHHHH
IAPGYDHITAAIGGAIAAFAGADFLCYVTPAEHLGLPDIQDVKEGVIAAKIAAHAADIAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIKKAREKDLAMAKARANLDWNEQIQLSIDPEKAEKYRVTKNKPNVETCSMCGKFCAMQI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHEECCCCCCCHHHHHHHHHHHHHHH
VSEYLGTPTTSC
HHHHHCCCCCCC
>Mature Secondary Structure 
TQLEYALSGIITKEMKIVADYEGVSEEFILEEVKKGEIVIPANVNHINLVPKGIGKGLS
CHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCEEEECCCCEEEECCHHCCCCCC
TKVNANIGTSDAFPEIGKEIEKLKVAIDAGADAVMDLSTGGDVNKSRREIIKNSSVPVGT
EEECCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
VPMYQAAVESISRYGSIVAMPEEFIFKVIEEQAKDGVDFITVHCGLTLESLKRLKDNKRI
CHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHH
MNVVSRGGAFTIAWMIHNEKENPLYQYFDRLLDIAKKYDVTLSLGDGLRPGCLEDATDAA
HHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHH
QIQELIILGELVKKAREAGVQVMVEGPGHVPIDQIEANVKLQKSLCHNAPFYVLGPVVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCCEEECHHHHH
IAPGYDHITAAIGGAIAAFAGADFLCYVTPAEHLGLPDIQDVKEGVIAAKIAAHAADIAK
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GIKKAREKDLAMAKARANLDWNEQIQLSIDPEKAEKYRVTKNKPNVETCSMCGKFCAMQI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHEECCCCCCCHHHHHHHHHHHHHHH
VSEYLGTPTTSC
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336