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The map label for this gene is TrpS

Identifier: 20807900

GI number: 20807900

Start: 1427418

End: 1428401

Strand: Reverse

Name: TrpS

Synonym: TTE1453

Alternate gene names: 20807900

Gene position: 1428401-1427418 (Counterclockwise)

Preceding gene: 20807901

Following gene: 20807897

Centisome position: 53.11

GC content: 42.07

Gene sequence:

>984_bases
TTGAAAAGAGTATTTTCTGGAGTACAGCCGTCAGGGGATATTCACATAGGAAATTACTTAGGTGCAATGAGACAGTTCGT
TGCCCTGCAGGATGACTACGATTGTTTCTTTTGTGTCGTGGATTTACACGCTCTTACAGTCCCTCAGGACCCGGTGGAAC
TTAAGAAAAATACCATTGAACTGGCAGCTTTATATATGGCAATAGGGCTTGACCCCAAAAAAGTCACTCTTTTTGTACAA
TCCCACGTTTCAGCTCATGCAGAACTGGCATGGCTGCTTCAATGCATCACTTATTTTGGAGAGCTTAGCAGAATGACTCA
GTTTAAAGAAAAGAGTAAGGGCAAAGAGTCGGTTTCTGTAGGACTTTTTACATACCCTGACTTGATGGCGGCCGACATAC
TTTTGTATAAGACCCATTATGTGCCTGTGGGAGAAGACCAAAAGCAGCATCTGGAGCTTACAAGAGATGTGGCACAGAGG
TTTAACAACAGGTTTGGGGAAACCTTTGTCATTCCAGAGCCCATGATCTTAAAGTTTGGCGCTAGAATTATGAGTTTGAC
AAACCCGACTAAAAAGATGAGCAAGAGCGACGCTGACCCCAATAACAGGGTAAATCTTTTGGATGACCCAGACACAATTT
ACAGGAAAATAATGAAAGCTGTGACAGATTCCGAAAGTGAAATAAGGCTTGATTGGGAGAAAAAACCGGGGATTAGCAAC
CTCCTCACAATATACAGCCTTTTCACTGGCATGGAAGTAGATGAAGTTGTGAATAAGTTTAAAGGGCAGGGTTACGGTAC
TTTGAAGAAGGAGCTAGCGGAGGTTGTCATTGATAAGCTTTCGGTTATACAGAAAAATTACAGGGATATAAGCGAAGAAG
AAGTTTTAAGGGTATTAAAAGAAGGGGCTGAAAGGGCAGAAGCTGTAGCAGTTGAGACCTTGAAAGAAGTAAAGGAAAAG
ATGGGGCTTATACTAAGAGATTAA

Upstream 100 bases:

>100_bases
ACCCCAATGCTTTTCTTTCCATACTTGATGTGTCAGAGGTTCACGGAAAGGGTTTCGTCAGAGAACTTTTTTAAATTCTT
CGCAAAGGAGGCGTTTGAGA

Downstream 100 bases:

>100_bases
AAGGCATTTAAAATGTGATTTACTTTTTCTAAAGTCAATTTTTCGGGATTGAGGTACACTTCAATTATGTCAAATCGCGG
TTGGTATTGTTTGAAGTTTG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKRVFSGVQPSGDIHIGNYLGAMRQFVALQDDYDCFFCVVDLHALTVPQDPVELKKNTIELAALYMAIGLDPKKVTLFVQ
SHVSAHAELAWLLQCITYFGELSRMTQFKEKSKGKESVSVGLFTYPDLMAADILLYKTHYVPVGEDQKQHLELTRDVAQR
FNNRFGETFVIPEPMILKFGARIMSLTNPTKKMSKSDADPNNRVNLLDDPDTIYRKIMKAVTDSESEIRLDWEKKPGISN
LLTIYSLFTGMEVDEVVNKFKGQGYGTLKKELAEVVIDKLSVIQKNYRDISEEEVLRVLKEGAERAEAVAVETLKEVKEK
MGLILRD

Sequences:

>Translated_327_residues
MKRVFSGVQPSGDIHIGNYLGAMRQFVALQDDYDCFFCVVDLHALTVPQDPVELKKNTIELAALYMAIGLDPKKVTLFVQ
SHVSAHAELAWLLQCITYFGELSRMTQFKEKSKGKESVSVGLFTYPDLMAADILLYKTHYVPVGEDQKQHLELTRDVAQR
FNNRFGETFVIPEPMILKFGARIMSLTNPTKKMSKSDADPNNRVNLLDDPDTIYRKIMKAVTDSESEIRLDWEKKPGISN
LLTIYSLFTGMEVDEVVNKFKGQGYGTLKKELAEVVIDKLSVIQKNYRDISEEEVLRVLKEGAERAEAVAVETLKEVKEK
MGLILRD
>Mature_327_residues
MKRVFSGVQPSGDIHIGNYLGAMRQFVALQDDYDCFFCVVDLHALTVPQDPVELKKNTIELAALYMAIGLDPKKVTLFVQ
SHVSAHAELAWLLQCITYFGELSRMTQFKEKSKGKESVSVGLFTYPDLMAADILLYKTHYVPVGEDQKQHLELTRDVAQR
FNNRFGETFVIPEPMILKFGARIMSLTNPTKKMSKSDADPNNRVNLLDDPDTIYRKIMKAVTDSESEIRLDWEKKPGISN
LLTIYSLFTGMEVDEVVNKFKGQGYGTLKKELAEVVIDKLSVIQKNYRDISEEEVLRVLKEGAERAEAVAVETLKEVKEK
MGLILRD

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=327, Percent_Identity=43.1192660550459, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI41352700, Length=177, Percent_Identity=51.4124293785311, Blast_Score=201, Evalue=9e-52,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=55.4878048780488, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71982800, Length=329, Percent_Identity=37.3860182370821, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI71982793, Length=329, Percent_Identity=37.3860182370821, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=39.5348837209302, Blast_Score=222, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24666151, Length=326, Percent_Identity=44.1717791411043, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_THETN (Q8R9X8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623071.1
- ProteinModelPortal:   Q8R9X8
- SMR:   Q8R9X8
- GeneID:   997893
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1453
- NMPDR:   fig|273068.3.peg.1418
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BioCyc:   TTEN273068:TTE1453-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37092; Mature: 37092

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVFSGVQPSGDIHIGNYLGAMRQFVALQDDYDCFFCVVDLHALTVPQDPVELKKNTIE
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEHEEHHHHHCCCCCHHHHHHHHHH
LAALYMAIGLDPKKVTLFVQSHVSAHAELAWLLQCITYFGELSRMTQFKEKSKGKESVSV
HHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEE
GLFTYPDLMAADILLYKTHYVPVGEDQKQHLELTRDVAQRFNNRFGETFVIPEPMILKFG
EEEECCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHH
ARIMSLTNPTKKMSKSDADPNNRVNLLDDPDTIYRKIMKAVTDSESEIRLDWEKKPGISN
HHHHHHCCHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
LLTIYSLFTGMEVDEVVNKFKGQGYGTLKKELAEVVIDKLSVIQKNYRDISEEEVLRVLK
HHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EGAERAEAVAVETLKEVKEKMGLILRD
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRVFSGVQPSGDIHIGNYLGAMRQFVALQDDYDCFFCVVDLHALTVPQDPVELKKNTIE
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEHEEHHHHHCCCCCHHHHHHHHHH
LAALYMAIGLDPKKVTLFVQSHVSAHAELAWLLQCITYFGELSRMTQFKEKSKGKESVSV
HHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEE
GLFTYPDLMAADILLYKTHYVPVGEDQKQHLELTRDVAQRFNNRFGETFVIPEPMILKFG
EEEECCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHH
ARIMSLTNPTKKMSKSDADPNNRVNLLDDPDTIYRKIMKAVTDSESEIRLDWEKKPGISN
HHHHHHCCHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHH
LLTIYSLFTGMEVDEVVNKFKGQGYGTLKKELAEVVIDKLSVIQKNYRDISEEEVLRVLK
HHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EGAERAEAVAVETLKEVKEKMGLILRD
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336