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The map label for this gene is FliM [H]

Identifier: 20807877

GI number: 20807877

Start: 1408191

End: 1409183

Strand: Reverse

Name: FliM [H]

Synonym: TTE1430

Alternate gene names: 20807877

Gene position: 1409183-1408191 (Counterclockwise)

Preceding gene: 20807878

Following gene: 20807876

Centisome position: 52.4

GC content: 37.97

Gene sequence:

>993_bases
TTGGCAGAGATACTTTCACAGAGTGAAATAGATGAGCTTTTAAAGGCTTTTAGTCAAGGGGAAGTAAAAGACCTAGAGAA
AGTAGAAGAGAAAAAGACAAAAAAAATTCGATCCTACGATTTCAGACGGCCCAACAAATTTTCAAAAGAACAGCTCAGAA
CCTTACAGATGATTTTTGAAAATTTTTCAAGGTCTCTCACGTCTTTTCTATCGGGCTATTTGAGGACCATTGTGAATGTG
GCTGTTGTATCTGTGGACCAGCTTACATATTACGAATTCAGCAATTCTTTAAACAATCCTGTGTTCATCGCTATAATTGA
CGCATCCCCTCTGGAAGGGCCATTCCTCTTAGAACTTAACAACAATACCACTTACGCCATATTGGATAGGCTCTTAGGAG
GGATGGGCAAAGGAGAATTTTTAGACAGAGATTATACAGAAATAGAAGTCAATTTGCTCACCAAAATTGTAAAGCAGATA
CTGCCTTTGTTAGAAGAGCCTTGGAGCAATATAATTGACCTCACTCCTTCCCTGATGAGAGTGGAGACCAATTCTCAGTT
TGCGCAGATCATTTCGCCAAATGAGACAGTGGCTTTATGCACGCTTTCAATAAAGATCAACGAGACAGAGGGAATGATGA
ATTTTTGCATGCCTCATCTGACGCTGGAGCCAATTGTGCCAAAACTTACAACTAAGTTTTGGTTTTCGAGCATGAAAAAA
GAAGAAAAAGTGAATCCCGAACTTATAAAAGAGAAGATTGACAAGACTTACATTCCTTTGACAGTGCAATTGGGAAGCAC
AACCGTCACGGTAGGAGACCTTTTAAAGTTTGAAGTAGGAGATGTGATAATGCTGGATAAAAGATACAACGAACCTGTAG
AGGTGATAATAGATAAAAAAGTAAAATTTAAAGGAATACCTGGAATTAAAAGTAAGCGATACAGCGTCAAAATAACAGAA
GTTATTTACAAAGGAGATGAAGAAAGTAAATGA

Upstream 100 bases:

>100_bases
AAATGACATAAAAAATGAGATAAACAAAATAATAGGCGAAGGAAAAGTAGTTAATGTGTACTTTAACGACTTTATCGTGC
AGTAGGGAGGAGGGGGTCTT

Downstream 100 bases:

>100_bases
GTTCTTGTCGCAAGAGGAAATAAACGCTCTCTTAAAAGGGCTCCAAGAAGAAACACAGGAAAAAAAAGAACTGACTGATG
AAGAAAAGGACATATTGGGG

Product: flagellar motor switch protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAEILSQSEIDELLKAFSQGEVKDLEKVEEKKTKKIRSYDFRRPNKFSKEQLRTLQMIFENFSRSLTSFLSGYLRTIVNV
AVVSVDQLTYYEFSNSLNNPVFIAIIDASPLEGPFLLELNNNTTYAILDRLLGGMGKGEFLDRDYTEIEVNLLTKIVKQI
LPLLEEPWSNIIDLTPSLMRVETNSQFAQIISPNETVALCTLSIKINETEGMMNFCMPHLTLEPIVPKLTTKFWFSSMKK
EEKVNPELIKEKIDKTYIPLTVQLGSTTVTVGDLLKFEVGDVIMLDKRYNEPVEVIIDKKVKFKGIPGIKSKRYSVKITE
VIYKGDEESK

Sequences:

>Translated_330_residues
MAEILSQSEIDELLKAFSQGEVKDLEKVEEKKTKKIRSYDFRRPNKFSKEQLRTLQMIFENFSRSLTSFLSGYLRTIVNV
AVVSVDQLTYYEFSNSLNNPVFIAIIDASPLEGPFLLELNNNTTYAILDRLLGGMGKGEFLDRDYTEIEVNLLTKIVKQI
LPLLEEPWSNIIDLTPSLMRVETNSQFAQIISPNETVALCTLSIKINETEGMMNFCMPHLTLEPIVPKLTTKFWFSSMKK
EEKVNPELIKEKIDKTYIPLTVQLGSTTVTVGDLLKFEVGDVIMLDKRYNEPVEVIIDKKVKFKGIPGIKSKRYSVKITE
VIYKGDEESK
>Mature_329_residues
AEILSQSEIDELLKAFSQGEVKDLEKVEEKKTKKIRSYDFRRPNKFSKEQLRTLQMIFENFSRSLTSFLSGYLRTIVNVA
VVSVDQLTYYEFSNSLNNPVFIAIIDASPLEGPFLLELNNNTTYAILDRLLGGMGKGEFLDRDYTEIEVNLLTKIVKQIL
PLLEEPWSNIIDLTPSLMRVETNSQFAQIISPNETVALCTLSIKINETEGMMNFCMPHLTLEPIVPKLTTKFWFSSMKKE
EKVNPELIKEKIDKTYIPLTVQLGSTTVTVGDLLKFEVGDVIMLDKRYNEPVEVIIDKKVKFKGIPGIKSKRYSVKITEV
IYKGDEESK

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=328, Percent_Identity=25.9146341463415, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37759; Mature: 37628

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEILSQSEIDELLKAFSQGEVKDLEKVEEKKTKKIRSYDFRRPNKFSKEQLRTLQMIFE
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
NFSRSLTSFLSGYLRTIVNVAVVSVDQLTYYEFSNSLNNPVFIAIIDASPLEGPFLLELN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEC
NNTTYAILDRLLGGMGKGEFLDRDYTEIEVNLLTKIVKQILPLLEEPWSNIIDLTPSLMR
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEE
VETNSQFAQIISPNETVALCTLSIKINETEGMMNFCMPHLTLEPIVPKLTTKFWFSSMKK
EECCCHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EEKVNPELIKEKIDKTYIPLTVQLGSTTVTVGDLLKFEVGDVIMLDKRYNEPVEVIIDKK
HHCCCHHHHHHHHCCCEEEEEEEECCEEEEHHHHHHCCCCCEEEECCCCCCCEEEEEECC
VKFKGIPGIKSKRYSVKITEVIYKGDEESK
EEECCCCCCCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
AEILSQSEIDELLKAFSQGEVKDLEKVEEKKTKKIRSYDFRRPNKFSKEQLRTLQMIFE
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
NFSRSLTSFLSGYLRTIVNVAVVSVDQLTYYEFSNSLNNPVFIAIIDASPLEGPFLLELN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEC
NNTTYAILDRLLGGMGKGEFLDRDYTEIEVNLLTKIVKQILPLLEEPWSNIIDLTPSLMR
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEE
VETNSQFAQIISPNETVALCTLSIKINETEGMMNFCMPHLTLEPIVPKLTTKFWFSSMKK
EECCCHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EEKVNPELIKEKIDKTYIPLTVQLGSTTVTVGDLLKFEVGDVIMLDKRYNEPVEVIIDKK
HHCCCHHHHHHHHCCCEEEEEEEECCEEEEHHHHHHCCCCCEEEECCCCCCCEEEEEECC
VKFKGIPGIKSKRYSVKITEVIYKGDEESK
EEECCCCCCCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]