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The map label for this gene is FlhF [H]

Identifier: 20807868

GI number: 20807868

Start: 1400501

End: 1401526

Strand: Reverse

Name: FlhF [H]

Synonym: TTE1421

Alternate gene names: 20807868

Gene position: 1401526-1400501 (Counterclockwise)

Preceding gene: 20807869

Following gene: 20807867

Centisome position: 52.11

GC content: 37.82

Gene sequence:

>1026_bases
GTGAAAATTAAAAAATACATAGCCGATGACTTTCAAGAGGCTTTAAAGATGATAAAGGCGGAAATGGGGAGCAATGCTGT
AATCCTTCATCAGCACAGGATAAGGGAAAGGGGAATAAAAGGGCTTTTTAAGAAGAGCAAAGTAGAGGTGGTGGCTGCTG
TAGAAGATAATCCTTACAATTTTCCCAAGGAAGTGATTACAAAAAGCGATATAAATGAACTTAAGAATCTGATAAAGACG
ATAGCTACGAATAAGAGCAGTGAAATTTCTGAAAATGGTGTAAAGTCTATCACTTTAAAGCTTGTGGAATATGGCATTGA
AAAAGAGTTATCACAGCTTTTGGGAGAAGGAATTGATAAATTGGACGATGTAGGAAAAGAAGTTTTGAAAAAGAGGATAA
AAGCCTTCTTGGGAGCCCCTCAGCCACTTGATAGCGAAAGAAGTAAAAAGGTGCTCTTCATAGGGCCTACTGGAGTGGGG
AAAACTACGACCGTCGCTAAAATTGCCTCTCATCTTATTTTGAACGAGGGGAAAAAAGTGATGCTTGTGACAGCGGATGT
CTTTAGAATTGCTGCTGTGGACCAACTTAAGAGTTATGGAGATATTTTAGGGGTTCCTGTAAAAGTTGTGGGCAATATAT
TTGAATTTCACAAACTTCAGCCCGAATTTGAAGATTATGATGTGGTTTTGATAGATACTGCTGGGAGAAGTCATAAAGAC
GAAAAGAGGATAAACGAACTTAAGACCTTTGTAAAATACGCTGAATGCGATGAAATTTTCCTCTGCTTAAGCGCTACTAC
CAGGTCTCAAGACTTAAAAGAAGTTATAAATAGATACGACTTTGCAGGCAATTACAAGTTGATATTCACAAAACTTGATG
AAACAGACAACTACAGTTCTATATTAAATGCCGTGTATTACTCTGGGCGACCAATTTCTTATTTTACAAACGGGCAAATA
GTACCTGATGACTTGATGTTAGCGGATGGGGAAATCATCTTTTCTAACATAGTAAAGGGGAATTGA

Upstream 100 bases:

>100_bases
AGAAAACTGGTGGAACAAATTTCTCCGGACATTGTTGTGCTGTCTTACAATGAGCTTTTGCCTAATATAGAAGTAATTTC
TGTGGGGACGGTGAAACTGG

Downstream 100 bases:

>100_bases
AATGATGGACCAGGCTGAAAGTTTAAGAAGATTGGTTTCCCAAAAGAAAGGCATAAAAAAAAGCCGAGTTATAACTATAA
GTGGCGGAAAAGGAGGGATA

Product: flagellar GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKIKKYIADDFQEALKMIKAEMGSNAVILHQHRIRERGIKGLFKKSKVEVVAAVEDNPYNFPKEVITKSDINELKNLIKT
IATNKSSEISENGVKSITLKLVEYGIEKELSQLLGEGIDKLDDVGKEVLKKRIKAFLGAPQPLDSERSKKVLFIGPTGVG
KTTTVAKIASHLILNEGKKVMLVTADVFRIAAVDQLKSYGDILGVPVKVVGNIFEFHKLQPEFEDYDVVLIDTAGRSHKD
EKRINELKTFVKYAECDEIFLCLSATTRSQDLKEVINRYDFAGNYKLIFTKLDETDNYSSILNAVYYSGRPISYFTNGQI
VPDDLMLADGEIIFSNIVKGN

Sequences:

>Translated_341_residues
MKIKKYIADDFQEALKMIKAEMGSNAVILHQHRIRERGIKGLFKKSKVEVVAAVEDNPYNFPKEVITKSDINELKNLIKT
IATNKSSEISENGVKSITLKLVEYGIEKELSQLLGEGIDKLDDVGKEVLKKRIKAFLGAPQPLDSERSKKVLFIGPTGVG
KTTTVAKIASHLILNEGKKVMLVTADVFRIAAVDQLKSYGDILGVPVKVVGNIFEFHKLQPEFEDYDVVLIDTAGRSHKD
EKRINELKTFVKYAECDEIFLCLSATTRSQDLKEVINRYDFAGNYKLIFTKLDETDNYSSILNAVYYSGRPISYFTNGQI
VPDDLMLADGEIIFSNIVKGN
>Mature_341_residues
MKIKKYIADDFQEALKMIKAEMGSNAVILHQHRIRERGIKGLFKKSKVEVVAAVEDNPYNFPKEVITKSDINELKNLIKT
IATNKSSEISENGVKSITLKLVEYGIEKELSQLLGEGIDKLDDVGKEVLKKRIKAFLGAPQPLDSERSKKVLFIGPTGVG
KTTTVAKIASHLILNEGKKVMLVTADVFRIAAVDQLKSYGDILGVPVKVVGNIFEFHKLQPEFEDYDVVLIDTAGRSHKD
EKRINELKTFVKYAECDEIFLCLSATTRSQDLKEVINRYDFAGNYKLIFTKLDETDNYSSILNAVYYSGRPISYFTNGQI
VPDDLMLADGEIIFSNIVKGN

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1788963, Length=180, Percent_Identity=32.7777777777778, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789874, Length=189, Percent_Identity=30.1587301587302, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325345, Length=281, Percent_Identity=27.4021352313167, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17647949, Length=224, Percent_Identity=27.2321428571429, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 38233; Mature: 38233

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKKYIADDFQEALKMIKAEMGSNAVILHQHRIRERGIKGLFKKSKVEVVAAVEDNPYN
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
FPKEVITKSDINELKNLIKTIATNKSSEISENGVKSITLKLVEYGIEKELSQLLGEGIDK
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDVGKEVLKKRIKAFLGAPQPLDSERSKKVLFIGPTGVGKTTTVAKIASHLILNEGKKV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
MLVTADVFRIAAVDQLKSYGDILGVPVKVVGNIFEFHKLQPEFEDYDVVLIDTAGRSHKD
EEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
EKRINELKTFVKYAECDEIFLCLSATTRSQDLKEVINRYDFAGNYKLIFTKLDETDNYSS
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
ILNAVYYSGRPISYFTNGQIVPDDLMLADGEIIFSNIVKGN
HHHHHHHCCCCEEEECCCEECCCCCEEECCHHHHHHHCCCC
>Mature Secondary Structure
MKIKKYIADDFQEALKMIKAEMGSNAVILHQHRIRERGIKGLFKKSKVEVVAAVEDNPYN
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
FPKEVITKSDINELKNLIKTIATNKSSEISENGVKSITLKLVEYGIEKELSQLLGEGIDK
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDVGKEVLKKRIKAFLGAPQPLDSERSKKVLFIGPTGVGKTTTVAKIASHLILNEGKKV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
MLVTADVFRIAAVDQLKSYGDILGVPVKVVGNIFEFHKLQPEFEDYDVVLIDTAGRSHKD
EEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
EKRINELKTFVKYAECDEIFLCLSATTRSQDLKEVINRYDFAGNYKLIFTKLDETDNYSS
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
ILNAVYYSGRPISYFTNGQIVPDDLMLADGEIIFSNIVKGN
HHHHHHHCCCCEEEECCCEECCCCCEEECCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1447978; 9384377 [H]