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The map label for this gene is FliA3 [H]

Identifier: 20807860

GI number: 20807860

Start: 1394188

End: 1394928

Strand: Reverse

Name: FliA3 [H]

Synonym: TTE1413

Alternate gene names: 20807860

Gene position: 1394928-1394188 (Counterclockwise)

Preceding gene: 20807861

Following gene: 20807859

Centisome position: 51.87

GC content: 37.52

Gene sequence:

>741_bases
ATGTCAGTAGTGGAGGAGGATGTTTGGAAAGTTTACAACCTTCAAAAAGACCCTGATGCGAGAAATGAGATTATTGTCAA
ATACCTTCCTTTGGTAAAACATATAGTGAAAAAGCTTGTAATATCAGATATCGCCCAGTCAGAGTATGAAGACCTTATCC
ACCAAGGGATTTTAGGACTCATAGATGCTATTGAAAAGTATCAGCCTGACAAAGGGGTAAAATTTGAGACATACGCTACT
TTGAGGATAAGAGGCGAAATAATAGATTACCTGAGAAAAAGGGACTGGATGCCGAGGAGTTTGAAGAAAAAGTACAAGAA
AATTGAAGAGACGGTAGAGAAGCTTCAGCAAAGGTACAACAGAGAGCCTACAGTGGAAGAAATTACAGAAGCTTGTGGAT
TAGAGGAAGAAGACGTGCTAAAGACTTTGAGCTACATAAATGTCTCAAACATTAATTCCCTTGAAGAAGCCATTGAGAAT
AATTTAAAGATTCACTCTATTTCAGAGATAGACCTGAAAAATCCTGAAGAGGAGCTTCTTTACAAAGAACTAAAGAAAAA
GCTCGCCCATGCGATTGAGAAGCTTTCTGAAAAAGAAAGACTGGTAATTACTCTGTACTACTATGAAGACTTAAACTACA
AGGATATAAGCAAGATACTCAATTTGACGGAGTCGCGCATTTCTCAAATTCATTCCAAAGCTATAAAAAAGATAAGGGAG
ATGCTGGGCGAGTACTTTTAA

Upstream 100 bases:

>100_bases
GAAGGACTATAGAATTTTATTCTGAAGACGGCAGACTTTTAGTAAAGACTATAGGCCATGGAGTCAAGTACATTTAAAAT
AAAAAACTGGGGGAATTCAT

Downstream 100 bases:

>100_bases
ATGGGGGCTTTGAAATGGATGAAAAAAGGTATAGGGTGTTAGTAGAGGTTTCAAAGGACAAGCAGCAGGCTTACATTTCT
CTAATTGAAGACCCCAATGG

Product: DNA-directed RNA polymerase specialized sigma subunit

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVVEEDVWKVYNLQKDPDARNEIIVKYLPLVKHIVKKLVISDIAQSEYEDLIHQGILGLIDAIEKYQPDKGVKFETYAT
LRIRGEIIDYLRKRDWMPRSLKKKYKKIEETVEKLQQRYNREPTVEEITEACGLEEEDVLKTLSYINVSNINSLEEAIEN
NLKIHSISEIDLKNPEEELLYKELKKKLAHAIEKLSEKERLVITLYYYEDLNYKDISKILNLTESRISQIHSKAIKKIRE
MLGEYF

Sequences:

>Translated_246_residues
MSVVEEDVWKVYNLQKDPDARNEIIVKYLPLVKHIVKKLVISDIAQSEYEDLIHQGILGLIDAIEKYQPDKGVKFETYAT
LRIRGEIIDYLRKRDWMPRSLKKKYKKIEETVEKLQQRYNREPTVEEITEACGLEEEDVLKTLSYINVSNINSLEEAIEN
NLKIHSISEIDLKNPEEELLYKELKKKLAHAIEKLSEKERLVITLYYYEDLNYKDISKILNLTESRISQIHSKAIKKIRE
MLGEYF
>Mature_245_residues
SVVEEDVWKVYNLQKDPDARNEIIVKYLPLVKHIVKKLVISDIAQSEYEDLIHQGILGLIDAIEKYQPDKGVKFETYATL
RIRGEIIDYLRKRDWMPRSLKKKYKKIEETVEKLQQRYNREPTVEEITEACGLEEEDVLKTLSYINVSNINSLEEAIENN
LKIHSISEIDLKNPEEELLYKELKKKLAHAIEKLSEKERLVITLYYYEDLNYKDISKILNLTESRISQIHSKAIKKIREM
LGEYF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=219, Percent_Identity=38.8127853881278, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789098, Length=237, Percent_Identity=26.5822784810127, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1789448, Length=230, Percent_Identity=26.9565217391304, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29105; Mature: 28974

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVEEDVWKVYNLQKDPDARNEIIVKYLPLVKHIVKKLVISDIAQSEYEDLIHQGILGL
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDAIEKYQPDKGVKFETYATLRIRGEIIDYLRKRDWMPRSLKKKYKKIEETVEKLQQRYN
HHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
REPTVEEITEACGLEEEDVLKTLSYINVSNINSLEEAIENNLKIHSISEIDLKNPEEELL
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
YKELKKKLAHAIEKLSEKERLVITLYYYEDLNYKDISKILNLTESRISQIHSKAIKKIRE
HHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGEYF
HHHCCC
>Mature Secondary Structure 
SVVEEDVWKVYNLQKDPDARNEIIVKYLPLVKHIVKKLVISDIAQSEYEDLIHQGILGL
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDAIEKYQPDKGVKFETYATLRIRGEIIDYLRKRDWMPRSLKKKYKKIEETVEKLQQRYN
HHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
REPTVEEITEACGLEEEDVLKTLSYINVSNINSLEEAIENNLKIHSISEIDLKNPEEELL
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
YKELKKKLAHAIEKLSEKERLVITLYYYEDLNYKDISKILNLTESRISQIHSKAIKKIRE
HHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGEYF
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]