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The map label for this gene is ispG

Identifier: 20807847

GI number: 20807847

Start: 1384628

End: 1385695

Strand: Reverse

Name: ispG

Synonym: TTE1400

Alternate gene names: 20807847

Gene position: 1385695-1384628 (Counterclockwise)

Preceding gene: 20807848

Following gene: 20807846

Centisome position: 51.52

GC content: 39.04

Gene sequence:

>1068_bases
GTGATAAAGATGAGGAAATTGACCCGCGAAATCAAGATAGGAAATAAGAAAATAGGAGGGAATAACCCGATCCTCGTTCA
GTCAATGACAAACACTGATACTCACGATGTGGAAAAGACGGTAGAGCAGATAAAGAGGCTGGAAGAAGAAGGATGTGATA
TAATAAGGGTAGCTGTACCAGATGAAAAGGCTGCTTACTCTATAAAAGAAATAAAAAAGCATATAAATATCCCTTTGGTG
GCAGACATACACTTTGACTACAGATTGGCTATTAAATCTATAGAAAATGGCGCTGATAAAATACGAATAAATCCCGGAAA
TATCGGAAGGGAAGAAAATATAAAAAAAGTAGTAGAAGCTGCAAAGGAAAGGGGTATACCTATACGGATAGGAGTAAATG
CAGGGTCTTTAGAAAAGGAGATATTAAATAAATACGGCGGTATAACCCCTGAAGCGGTAGTAGAGAGCGCTTTAAAAAGT
GTGCGCCTTTTAGAAAAACTTGGCTTTTACGATATAGTGATTTCTTTAAAAACTTCCAATGTGCCCCTTACAATAGAAGC
ATATAAATTAGCTTCTTCCAAGGTGGACTATCCACTCCACCTTGGAATTACAGAAGCTGGAACTTTGGAAAGCGGAACTA
TTAAATCAGCGATCGGCATAGGCACTCTTCTTTACATGGGAATAGGGGATACAATAAGGGTTTCTTTGACAGGCGATCCC
GTGCACGAAGTTAGGGTAGGAAGACAGATTTTGCGCGCTTTAGGACTGCTCAAAGAAGGGGTAGAGGTCATTTCATGCCC
TACCTGTGGCAGGACAAAAATAGATGTCATTAAATTAGCTACTGAGGTTGAAAGAAGAACATCTCATATAAAAAAACCTC
TTAAAGTGGCTGTCATGGGCTGTGTGGTAAATGGACCTGGAGAAGCAAAAGAAGCAGACATTGGAATTGCAGGAGGAGAT
AGAGAAGGGGTTATATTCAAAAAAGGAAAGATATACAAGATAGTTAAAGAAGAACACCTTTTAGAAGAACTTCTCAAAGA
AATTGAGAAGATGGTCAAGGAGGAATAA

Upstream 100 bases:

>100_bases
CGGAAAAAGAAGGTTATATCCATTACTTAGGCTTCTTGCTGTTGATCGCTTTGCTCATATTTGCTACTTACAGGGATATA
ATGCGAATTTTCTAAATTAG

Downstream 100 bases:

>100_bases
AAATAATGATTTCTGTCATCGTGCCTGCTTACAATGAAGGGAAAAACATTGGAAGAGTACTTTCTGTCCTTGAGAAAATT
GATGTGATAGACGAAATTAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIKMRKLTREIKIGNKKIGGNNPILVQSMTNTDTHDVEKTVEQIKRLEEEGCDIIRVAVPDEKAAYSIKEIKKHINIPLV
ADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEAAKERGIPIRIGVNAGSLEKEILNKYGGITPEAVVESALKS
VRLLEKLGFYDIVISLKTSNVPLTIEAYKLASSKVDYPLHLGITEAGTLESGTIKSAIGIGTLLYMGIGDTIRVSLTGDP
VHEVRVGRQILRALGLLKEGVEVISCPTCGRTKIDVIKLATEVERRTSHIKKPLKVAVMGCVVNGPGEAKEADIGIAGGD
REGVIFKKGKIYKIVKEEHLLEELLKEIEKMVKEE

Sequences:

>Translated_355_residues
MIKMRKLTREIKIGNKKIGGNNPILVQSMTNTDTHDVEKTVEQIKRLEEEGCDIIRVAVPDEKAAYSIKEIKKHINIPLV
ADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEAAKERGIPIRIGVNAGSLEKEILNKYGGITPEAVVESALKS
VRLLEKLGFYDIVISLKTSNVPLTIEAYKLASSKVDYPLHLGITEAGTLESGTIKSAIGIGTLLYMGIGDTIRVSLTGDP
VHEVRVGRQILRALGLLKEGVEVISCPTCGRTKIDVIKLATEVERRTSHIKKPLKVAVMGCVVNGPGEAKEADIGIAGGD
REGVIFKKGKIYKIVKEEHLLEELLKEIEKMVKEE
>Mature_355_residues
MIKMRKLTREIKIGNKKIGGNNPILVQSMTNTDTHDVEKTVEQIKRLEEEGCDIIRVAVPDEKAAYSIKEIKKHINIPLV
ADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEAAKERGIPIRIGVNAGSLEKEILNKYGGITPEAVVESALKS
VRLLEKLGFYDIVISLKTSNVPLTIEAYKLASSKVDYPLHLGITEAGTLESGTIKSAIGIGTLLYMGIGDTIRVSLTGDP
VHEVRVGRQILRALGLLKEGVEVISCPTCGRTKIDVIKLATEVERRTSHIKKPLKVAVMGCVVNGPGEAKEADIGIAGGD
REGVIFKKGKIYKIVKEEHLLEELLKEIEKMVKEE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=328, Percent_Identity=53.6585365853659, Blast_Score=356, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_THETN (Q8RA30)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623018.1
- ProteinModelPortal:   Q8RA30
- GeneID:   997868
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1400
- NMPDR:   fig|273068.3.peg.1365
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   TTEN273068:TTE1400-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 39006; Mature: 39006

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMRKLTREIKIGNKKIGGNNPILVQSMTNTDTHDVEKTVEQIKRLEEEGCDIIRVAVP
CCCHHHHHHHHHCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
DEKAAYSIKEIKKHINIPLVADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEA
CCHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
AKERGIPIRIGVNAGSLEKEILNKYGGITPEAVVESALKSVRLLEKLGFYDIVISLKTSN
HHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
VPLTIEAYKLASSKVDYPLHLGITEAGTLESGTIKSAIGIGTLLYMGIGDTIRVSLTGDP
CCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
VHEVRVGRQILRALGLLKEGVEVISCPTCGRTKIDVIKLATEVERRTSHIKKPLKVAVMG
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
CVVNGPGEAKEADIGIAGGDREGVIFKKGKIYKIVKEEHLLEELLKEIEKMVKEE
EEECCCCCCCCCCEEEECCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKMRKLTREIKIGNKKIGGNNPILVQSMTNTDTHDVEKTVEQIKRLEEEGCDIIRVAVP
CCCHHHHHHHHHCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
DEKAAYSIKEIKKHINIPLVADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEA
CCHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
AKERGIPIRIGVNAGSLEKEILNKYGGITPEAVVESALKSVRLLEKLGFYDIVISLKTSN
HHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
VPLTIEAYKLASSKVDYPLHLGITEAGTLESGTIKSAIGIGTLLYMGIGDTIRVSLTGDP
CCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
VHEVRVGRQILRALGLLKEGVEVISCPTCGRTKIDVIKLATEVERRTSHIKKPLKVAVMG
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
CVVNGPGEAKEADIGIAGGDREGVIFKKGKIYKIVKEEHLLEELLKEIEKMVKEE
EEECCCCCCCCCCEEEECCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336