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The map label for this gene is truB

Identifier: 20807837

GI number: 20807837

Start: 1373262

End: 1374119

Strand: Reverse

Name: truB

Synonym: TTE1390

Alternate gene names: 20807837

Gene position: 1374119-1373262 (Counterclockwise)

Preceding gene: 20807838

Following gene: 20807836

Centisome position: 51.09

GC content: 36.95

Gene sequence:

>858_bases
ATGAGTGTGGACGGCGTTTTAAATGTGCTAAAACCTCCTGGCATGACTTCTCACGATGTGGTGGATTTTATAAGAAAAAT
TTACGGTATAAAGAAAGTGGGCCACACAGGAACTCTTGATCCAGATGCAGCTGGAGTTTTACCTGTATGCATGGGAAGAG
CTACTAAGTTCACTTCTTACCTGATGGAACATGACAAAAGGTACAGATTTGAAATAACTTTTGGTTTTTCTACTGATACC
CTAGATAAAAGTGGCAAAATCGTTGAAAGTGGCCCTGTTCCCTTATTTACTTTGGAAAAATTACAGGAAGTTTTAAGCCA
GTTTAAAGGGGAAATACAACAGATACCACCCATTTATTCTGCAAAAAAAGTAAAAGGAAAGAAGCTCTATGAATATGCGA
GAAAAGGCGAAGAAGTGGAAATTCCACCCATAAAAGTGACAGTTTATGAGTTAGAATTAATAAAATACGATGCTCCGCAC
CATCTGCTGTTAGATGTAAAGTGTTCCAAAGGCACTTATGTAAGAGCTCTTGTGAGAGATATATGCAAGAAACTTGAAGT
TCCAGGCCACATGTCTTTTCTCATAAGGACAGAGGTCGGAGACTTTGACATTGAATCTTCATATACGCTGGAGGAGATAA
AGGAGGGAAAGGCAGAAGTACAGCCCGTAGATAAGTTTATAAAATTTCCTTCCGTGGAACTTGATGAAGTTTCTTCCAAT
AAAATTTTAAATGGCCAATTTATCAGAAATACTTACAATGTTGAAAATTCTTTAGTAAAGTTGTATGATAATCATGGAAT
TTTTATAGGAATTGGAGTGGCAGAAGGGGAAAAAATACGACCAAAAAGACTTTTCTAA

Upstream 100 bases:

>100_bases
GAGGAGGAGGGCATGTAAGGGCCTCTGGCTGTACAATAGAGGGGACTATCGAAGAAGCAAAAGAGAAAATTCTTAAAGAA
GTCTTTAAGGAGTTAGAAAA

Downstream 100 bases:

>100_bases
GGGGGAAAGAATTTTGATAATACTTGACGAAACCAATTTCTCAGCTAATCTAGAAGAAAAAGTGATAGCTCTTGGAAATT
TTGATGGAGTACACATTGGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSVDGVLNVLKPPGMTSHDVVDFIRKIYGIKKVGHTGTLDPDAAGVLPVCMGRATKFTSYLMEHDKRYRFEITFGFSTDT
LDKSGKIVESGPVPLFTLEKLQEVLSQFKGEIQQIPPIYSAKKVKGKKLYEYARKGEEVEIPPIKVTVYELELIKYDAPH
HLLLDVKCSKGTYVRALVRDICKKLEVPGHMSFLIRTEVGDFDIESSYTLEEIKEGKAEVQPVDKFIKFPSVELDEVSSN
KILNGQFIRNTYNVENSLVKLYDNHGIFIGIGVAEGEKIRPKRLF

Sequences:

>Translated_285_residues
MSVDGVLNVLKPPGMTSHDVVDFIRKIYGIKKVGHTGTLDPDAAGVLPVCMGRATKFTSYLMEHDKRYRFEITFGFSTDT
LDKSGKIVESGPVPLFTLEKLQEVLSQFKGEIQQIPPIYSAKKVKGKKLYEYARKGEEVEIPPIKVTVYELELIKYDAPH
HLLLDVKCSKGTYVRALVRDICKKLEVPGHMSFLIRTEVGDFDIESSYTLEEIKEGKAEVQPVDKFIKFPSVELDEVSSN
KILNGQFIRNTYNVENSLVKLYDNHGIFIGIGVAEGEKIRPKRLF
>Mature_284_residues
SVDGVLNVLKPPGMTSHDVVDFIRKIYGIKKVGHTGTLDPDAAGVLPVCMGRATKFTSYLMEHDKRYRFEITFGFSTDTL
DKSGKIVESGPVPLFTLEKLQEVLSQFKGEIQQIPPIYSAKKVKGKKLYEYARKGEEVEIPPIKVTVYELELIKYDAPHH
LLLDVKCSKGTYVRALVRDICKKLEVPGHMSFLIRTEVGDFDIESSYTLEEIKEGKAEVQPVDKFIKFPSVELDEVSSNK
ILNGQFIRNTYNVENSLVKLYDNHGIFIGIGVAEGEKIRPKRLF

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=36.9369369369369, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI215599015, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4503337, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI2367200, Length=300, Percent_Identity=41, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=29, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=147, Percent_Identity=40.1360544217687, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=216, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281364189, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364187, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364185, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281364183, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI62471759, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17975520, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_THETN (Q8RA40)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623008.1
- ProteinModelPortal:   Q8RA40
- SMR:   Q8RA40
- GeneID:   997292
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1390
- NMPDR:   fig|273068.3.peg.1355
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   PRK00130
- BioCyc:   TTEN273068:TTE1390-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 32189; Mature: 32058

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: ACT_SITE 40-40

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDGVLNVLKPPGMTSHDVVDFIRKIYGIKKVGHTGTLDPDAAGVLPVCMGRATKFTSY
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHH
LMEHDKRYRFEITFGFSTDTLDKSGKIVESGPVPLFTLEKLQEVLSQFKGEIQQIPPIYS
HHHCCCEEEEEEEECCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCC
AKKVKGKKLYEYARKGEEVEIPPIKVTVYELELIKYDAPHHLLLDVKCSKGTYVRALVRD
CHHCCHHHHHHHHHCCCEEECCCEEEEEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHH
ICKKLEVPGHMSFLIRTEVGDFDIESSYTLEEIKEGKAEVQPVDKFIKFPSVELDEVSSN
HHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
KILNGQFIRNTYNVENSLVKLYDNHGIFIGIGVAEGEKIRPKRLF
CEECCEEEECCCCCCHHEEEEECCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SVDGVLNVLKPPGMTSHDVVDFIRKIYGIKKVGHTGTLDPDAAGVLPVCMGRATKFTSY
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHHHHHH
LMEHDKRYRFEITFGFSTDTLDKSGKIVESGPVPLFTLEKLQEVLSQFKGEIQQIPPIYS
HHHCCCEEEEEEEECCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCC
AKKVKGKKLYEYARKGEEVEIPPIKVTVYELELIKYDAPHHLLLDVKCSKGTYVRALVRD
CHHCCHHHHHHHHHCCCEEECCCEEEEEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHH
ICKKLEVPGHMSFLIRTEVGDFDIESSYTLEEIKEGKAEVQPVDKFIKFPSVELDEVSSN
HHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCC
KILNGQFIRNTYNVENSLVKLYDNHGIFIGIGVAEGEKIRPKRLF
CEECCEEEECCCCCCHHEEEEECCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336