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The map label for this gene is Pnp

Identifier: 20807834

GI number: 20807834

Start: 1369755

End: 1371878

Strand: Reverse

Name: Pnp

Synonym: TTE1387

Alternate gene names: 20807834

Gene position: 1371878-1369755 (Counterclockwise)

Preceding gene: 20807835

Following gene: 20807833

Centisome position: 51.01

GC content: 42.61

Gene sequence:

>2124_bases
ATGGAAGAACGAACTTTTGAAATGGAACTAGCAGGCAGGAAGCTTGTAGTCCAGACCGGGAAAGTGGCACAGCAGGCCAA
TGGAGCAGCATGGGTGAAATACGGCGATACAGTTGTGCTGGTTACTGCCTGTGCTTCAAAAGAGCCGAGAGAAGGAATTG
ACTTTTTCCCCTTGACGGTTGAATACGAAGAGAGATTGTATTCCATTGGGAAAATTCCCGGCGGTTTTATCAAAAGAGAG
GGGAAGCCCAGCGAAAAAGCTATTTTATCAGCTCGGTTAATAGATAGACCTATAAGGCCTCTATTCCCAGAAGGATACAG
AAATGACGTGCAGGTCATAGCTACTGTGTTATCAGTGGACCCTGATGCGCAGCCAGAAATTGTAGCTATGATAGGTTCTT
CTGTTGCGCTCTCTATATCCGACATACCCTTTAACGGCCCCACAGGTTCTGTGGCAGTAGGCCTGGTGGACGGAAAGTTT
GTCATAAATCCCACCTATGAGCAGAGAGAAAAGAGCTTAATGCACTTAGTAGTTTCTGGAACAAAAGATGCAATTGTAAT
GGTGGAAGCTGGAGCGAAAGAAGTGCCTGAGGAGACAATACTTGATGCGATAGTGTATGCTCATCAATATATAAAACAGA
TTGTAGAATTTATAGAAGGGATTGTAAAAGAAGTAGGAGTTCCTAAGGCAGAAGTCGTACTGCACGAAATTGACAAGGAG
CTGGAGGAAAAAGTAAGAGCATATGCTACAGAGAAAATATATAACGCTTTAAGAACTCCTGAAAAGAAAGAGAGAAATGA
CAACTTAGACAAGGTAGAACAGGAAGTACTTGAGCACTTTAAAGATGAGTACCCCGATAATCTAGCAGATATTGACGAAG
TCCTCTACAAAATAATGAAAGAACAGATGAGAAAGATGATAAAGGAAGAAAAGATAAGGGTAGATGGAAGAGGGCTTGAC
GATATAAGGCCGATATGGTGCGAAGTAGGAGTACTGCCAAGAACTCACGGTTCTGCTATTTTTACAAGAGGACAAACTCA
AGTGCTCACAGTAGCTACTCTTGGAGCAATAGGTGATATACAGATTTTAGAAGGAATTGGAGATGAAGAATTCAAGCGTT
ACATGCACCATTACAACTTCCCACCCTATAGTGTAGGAGAAGTAAGGCCACTGAGAGGCCCTGGAAGAAGGGAAATTGGA
CACGGAGCCTTGGCAGAAAGGGCACTAGAACCAGTAATTCCTTCTGAAGAAGAATTCCCATACACAATACGCTTAGTGTC
TGAAGTATTGAGCTCTAACGGTTCTACATCACAGGCGAGTGTGTGCGGAAGCACTTTGGCTTTGATGGACGCGGGAGTGC
CAATCAAAGCACCGGTTGCTGGAATTGCAATGGGACTCATAAAAGAAGAAGATGAAGTACTAATTCTGACTGATATACAG
GGAATTGAGGACTTCCTCGGAGACATGGACTTTAAAGTAGCTGGAACAGAAAAAGGCGTAACTGCTATTCAGATGGATAT
CAAAATACCTGGCATTGATAGAGATATCCTGCAAATGGCTTTGGAAAAGGCAAGGAAGGCGAGACTATACGTGCTCCAAA
AGATGCTGGAAGTCATAAAAGAACCGAGGAAAGAACTCTCCAAGTATGCTCCTAGAGTAGTGAGAATGGTAGTCAACCCT
GAAAAGATAAGAGATATCATAGGCCCTGCAGGAAAGACTATCACAAAGATCATATCTGAGACAGGTGTCAAAATCGACAT
AGAAGAAGACGGTCGACTGTATATAACAGCTCCTAACCTTGAGGCCGGCGAAAGAGCTAAACAGATGATTGAAGCTATAA
CAAAGGACATAGAAGTTGGGGGAATCTATTTAGGAAAAGTGTTGAGAATCGCGCCTTTTGGAGCTTTTGTAGAGATCGCC
CCAGGTAAAGAAGGATTAGTTCACATTTCTAACTTGTCCAAAAAGAGAGTCCGCCGGGTAGAAGACGTAGTAAAAGTGGG
AGACAATATATTGGTTAAGGTAACAGAAATTGACAAACTCGGAAGAATTGTATTGTCTCGCAAAGACGCTATGCCTGATG
AAGAAGAAAGTGATAACAGGAAAAGTGATAATAGAAAAAAGTAA

Upstream 100 bases:

>100_bases
CGCGCTATAATTGAAAAATTAGACCTCAGAAAATAGAGCGACTTTTTCGCTCTATTTTCTGATAAAAATTTACAAATGAA
AGGAGGAAAATGAGGAAGGT

Downstream 100 bases:

>100_bases
AGGCATAAACCGCCCCCACGGTTTATGTTTTTTATTTTTTAGTAATATAAACGCCGCCTGGCTGAATAAAATTAGAATGA
GAATACCAGACAGGTGGTGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MEERTFEMELAGRKLVVQTGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTVEYEERLYSIGKIPGGFIKRE
GKPSEKAILSARLIDRPIRPLFPEGYRNDVQVIATVLSVDPDAQPEIVAMIGSSVALSISDIPFNGPTGSVAVGLVDGKF
VINPTYEQREKSLMHLVVSGTKDAIVMVEAGAKEVPEETILDAIVYAHQYIKQIVEFIEGIVKEVGVPKAEVVLHEIDKE
LEEKVRAYATEKIYNALRTPEKKERNDNLDKVEQEVLEHFKDEYPDNLADIDEVLYKIMKEQMRKMIKEEKIRVDGRGLD
DIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGAIGDIQILEGIGDEEFKRYMHHYNFPPYSVGEVRPLRGPGRREIG
HGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGIAMGLIKEEDEVLILTDIQ
GIEDFLGDMDFKVAGTEKGVTAIQMDIKIPGIDRDILQMALEKARKARLYVLQKMLEVIKEPRKELSKYAPRVVRMVVNP
EKIRDIIGPAGKTITKIISETGVKIDIEEDGRLYITAPNLEAGERAKQMIEAITKDIEVGGIYLGKVLRIAPFGAFVEIA
PGKEGLVHISNLSKKRVRRVEDVVKVGDNILVKVTEIDKLGRIVLSRKDAMPDEEESDNRKSDNRKK

Sequences:

>Translated_707_residues
MEERTFEMELAGRKLVVQTGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTVEYEERLYSIGKIPGGFIKRE
GKPSEKAILSARLIDRPIRPLFPEGYRNDVQVIATVLSVDPDAQPEIVAMIGSSVALSISDIPFNGPTGSVAVGLVDGKF
VINPTYEQREKSLMHLVVSGTKDAIVMVEAGAKEVPEETILDAIVYAHQYIKQIVEFIEGIVKEVGVPKAEVVLHEIDKE
LEEKVRAYATEKIYNALRTPEKKERNDNLDKVEQEVLEHFKDEYPDNLADIDEVLYKIMKEQMRKMIKEEKIRVDGRGLD
DIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGAIGDIQILEGIGDEEFKRYMHHYNFPPYSVGEVRPLRGPGRREIG
HGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGIAMGLIKEEDEVLILTDIQ
GIEDFLGDMDFKVAGTEKGVTAIQMDIKIPGIDRDILQMALEKARKARLYVLQKMLEVIKEPRKELSKYAPRVVRMVVNP
EKIRDIIGPAGKTITKIISETGVKIDIEEDGRLYITAPNLEAGERAKQMIEAITKDIEVGGIYLGKVLRIAPFGAFVEIA
PGKEGLVHISNLSKKRVRRVEDVVKVGDNILVKVTEIDKLGRIVLSRKDAMPDEEESDNRKSDNRKK
>Mature_707_residues
MEERTFEMELAGRKLVVQTGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTVEYEERLYSIGKIPGGFIKRE
GKPSEKAILSARLIDRPIRPLFPEGYRNDVQVIATVLSVDPDAQPEIVAMIGSSVALSISDIPFNGPTGSVAVGLVDGKF
VINPTYEQREKSLMHLVVSGTKDAIVMVEAGAKEVPEETILDAIVYAHQYIKQIVEFIEGIVKEVGVPKAEVVLHEIDKE
LEEKVRAYATEKIYNALRTPEKKERNDNLDKVEQEVLEHFKDEYPDNLADIDEVLYKIMKEQMRKMIKEEKIRVDGRGLD
DIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGAIGDIQILEGIGDEEFKRYMHHYNFPPYSVGEVRPLRGPGRREIG
HGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGIAMGLIKEEDEVLILTDIQ
GIEDFLGDMDFKVAGTEKGVTAIQMDIKIPGIDRDILQMALEKARKARLYVLQKMLEVIKEPRKELSKYAPRVVRMVVNP
EKIRDIIGPAGKTITKIISETGVKIDIEEDGRLYITAPNLEAGERAKQMIEAITKDIEVGGIYLGKVLRIAPFGAFVEIA
PGKEGLVHISNLSKKRVRRVEDVVKVGDNILVKVTEIDKLGRIVLSRKDAMPDEEESDNRKSDNRKK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=40, Blast_Score=484, Evalue=1e-136,
Organism=Escherichia coli, GI145693187, Length=678, Percent_Identity=49.7050147492625, Blast_Score=664, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=718, Percent_Identity=34.5403899721448, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI281362905, Length=703, Percent_Identity=40.5405405405405, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24651641, Length=703, Percent_Identity=40.5405405405405, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24651643, Length=703, Percent_Identity=40.5405405405405, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI161079377, Length=648, Percent_Identity=40.5864197530864, Blast_Score=445, Evalue=1e-125,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_THETN (Q8RA43)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623005.1
- HSSP:   P05055
- ProteinModelPortal:   Q8RA43
- GeneID:   995433
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1387
- NMPDR:   fig|273068.3.peg.1352
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   TTEN273068:TTE1387-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78521; Mature: 78521

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERTFEMELAGRKLVVQTGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTV
CCCCEEEHEECCCEEEEECCHHHHHCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEE
EYEERLYSIGKIPGGFIKREGKPSEKAILSARLIDRPIRPLFPEGYRNDVQVIATVLSVD
EHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDAQPEIVAMIGSSVALSISDIPFNGPTGSVAVGLVDGKFVINPTYEQREKSLMHLVVSG
CCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHEEC
TKDAIVMVEAGAKEVPEETILDAIVYAHQYIKQIVEFIEGIVKEVGVPKAEVVLHEIDKE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LEEKVRAYATEKIYNALRTPEKKERNDNLDKVEQEVLEHFKDEYPDNLADIDEVLYKIMK
HHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMRKMIKEEKIRVDGRGLDDIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGAIGDI
HHHHHHHHHHHEEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEEEECCCCCCH
QILEGIGDEEFKRYMHHYNFPPYSVGEVRPLRGPGRREIGHGALAERALEPVIPSEEEFP
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
YTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGIAMGLIKEEDEVLILTDIQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCH
GIEDFLGDMDFKVAGTEKGVTAIQMDIKIPGIDRDILQMALEKARKARLYVLQKMLEVIK
HHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EPRKELSKYAPRVVRMVVNPEKIRDIIGPAGKTITKIISETGVKIDIEEDGRLYITAPNL
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCC
EAGERAKQMIEAITKDIEVGGIYLGKVLRIAPFGAFVEIAPGKEGLVHISNLSKKRVRRV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCEEEHHHHHHHHHHHH
EDVVKVGDNILVKVTEIDKLGRIVLSRKDAMPDEEESDNRKSDNRKK
HHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEERTFEMELAGRKLVVQTGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTV
CCCCEEEHEECCCEEEEECCHHHHHCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEE
EYEERLYSIGKIPGGFIKREGKPSEKAILSARLIDRPIRPLFPEGYRNDVQVIATVLSVD
EHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDAQPEIVAMIGSSVALSISDIPFNGPTGSVAVGLVDGKFVINPTYEQREKSLMHLVVSG
CCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHEEC
TKDAIVMVEAGAKEVPEETILDAIVYAHQYIKQIVEFIEGIVKEVGVPKAEVVLHEIDKE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LEEKVRAYATEKIYNALRTPEKKERNDNLDKVEQEVLEHFKDEYPDNLADIDEVLYKIMK
HHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMRKMIKEEKIRVDGRGLDDIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGAIGDI
HHHHHHHHHHHEEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEEEECCCCCCH
QILEGIGDEEFKRYMHHYNFPPYSVGEVRPLRGPGRREIGHGALAERALEPVIPSEEEFP
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
YTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGIAMGLIKEEDEVLILTDIQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCH
GIEDFLGDMDFKVAGTEKGVTAIQMDIKIPGIDRDILQMALEKARKARLYVLQKMLEVIK
HHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EPRKELSKYAPRVVRMVVNPEKIRDIIGPAGKTITKIISETGVKIDIEEDGRLYITAPNL
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCC
EAGERAKQMIEAITKDIEVGGIYLGKVLRIAPFGAFVEIAPGKEGLVHISNLSKKRVRRV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCEEEHHHHHHHHHHHH
EDVVKVGDNILVKVTEIDKLGRIVLSRKDAMPDEEESDNRKSDNRKK
HHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336