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The map label for this gene is MiaB2

Identifier: 20807803

GI number: 20807803

Start: 1336868

End: 1338283

Strand: Direct

Name: MiaB2

Synonym: TTE1356

Alternate gene names: 20807803

Gene position: 1336868-1338283 (Clockwise)

Preceding gene: 20807802

Following gene: 20807804

Centisome position: 49.71

GC content: 38.21

Gene sequence:

>1416_bases
ATGCGTAATTCCTACTATGTTTCTGAAGAAGAGCTAAAAAAACAGAGAGAAATAATGGAAGAAATTGCTTGGGAAAACAG
AGGAAAAGAAGTGTATTATCACATTGAAACTTACGGATGCCAGATGAATGTGCACGACTCTGAAAAATTGGCTGGTATGC
TGGAGGAAATGGGCTATAAATATACAGAAGATTTAGAAAAAGCAGATGTTTTACTTTTTAATACTTGTGCTGTGAGAGAA
CACGCGGAGGTAAGGGTATTAGGAAGAGTGTCCCAGATAAAGGAACTAAAAAATAGAAATCCCAATCTCATCATTGGTAT
TTCTGGATGTATGATGCAGGAAAAGCATATAGTGGAGGCTATAAGAGAAAAGTATCCTCATGTAGATATTGTTTTTGGCA
CTCACAATATTTATAAATTCCCGGAACTTCTATGGCAAGCCTTAAATTCTCGTGTGCAAGTGATAGATGTGATTGAAAAT
ACTCAAAATGTAATTGAAGGGTTACCTATAAGAAGAGATAGCAATCTTAAAGCGTGGGTCAATATAATATACGGGTGCAA
CAATTTTTGCACCTACTGCATAGTTCCATACACGAGAGGAAGAGAAAAGAGCAGAAGGCCTGAAGACATAATAGCAGAGG
TAAAAGAACTAGCAGAAAAAGGGTACAAAGAGATCACATTATTGGGTCAAAATGTAAATTCATACGGAAAGGACCTGGGG
GGAGACATCAATTTTGCTAAGCTCCTTTATATGTTAAATGACATCAAAGGGATAGAGAGAATAAGGTTTATGACTTCCCA
CCCAAAGGATATTTCAGATGAATTAATATACGCTATGAGAGACCTCGACAAAGTATGTGAGCACCTCCATCTTCCTGTCC
AGGCGGGCAGCAATAAAATATTAAAAAGAATGAACAGGCGTTATACTAGAGAGCATTACCTGGAAATAATTGATAAAGTC
AGAGCCAATATTCCTGACATAGCAATTACAACAGACATAATTGTAGGATTTCCTGGAGAGACAGAAGAAGATTTCTTTCA
GACTCTTGATTTGGTAGAAAGAGTAAGGTTTGACGGCGCTTACACTTTTATGTACTCTAAAAGGGCAGGGACTCCAGCAG
CCTCCATGCCAGACCAGGTAGATGACGAAGTGAAAAAAGATCGTTTGATAAGGCTTATAGAGCTTCAGAACAAAATAAGC
CTTGAAAAAAGTGCAGAACTCAAAGGGAAAGTAGTAGAAGTCCTTATAGAAGGAGTCAGCAAAAGAGACCCCAATAAGCT
CACTTCACGCACTAGAACAAATAAAGTCGTCCATTTCATAGGAGACGAAAGTCTTATTGGCAAGTTGGCAAACGTGAAAA
TAACCGAGACTAAAGCCTGGACAATGCAAGGAGAATTGGTAGAAGTTCTAAGGTAA

Upstream 100 bases:

>100_bases
TACCGAGCAGATAATATTGCCTTATAGGTATAATTTTGAACCTGTCAGGACTTTTTTCTACAAAAAAATCAGTTTTCAAA
AATGAAGAGGTGTCATAAAG

Downstream 100 bases:

>100_bases
AACTGCGCTCTAGAAAGAGCGCAGTTTTATGGTATAATAAAGAGGAGTCGGAGGTGCTAAAAATGTCTGTAACTCCAATG
ATGGAACAGTACCTTAAAAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRNSYYVSEEELKKQREIMEEIAWENRGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYKYTEDLEKADVLLFNTCAVRE
HAEVRVLGRVSQIKELKNRNPNLIIGISGCMMQEKHIVEAIREKYPHVDIVFGTHNIYKFPELLWQALNSRVQVIDVIEN
TQNVIEGLPIRRDSNLKAWVNIIYGCNNFCTYCIVPYTRGREKSRRPEDIIAEVKELAEKGYKEITLLGQNVNSYGKDLG
GDINFAKLLYMLNDIKGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKILKRMNRRYTREHYLEIIDKV
RANIPDIAITTDIIVGFPGETEEDFFQTLDLVERVRFDGAYTFMYSKRAGTPAASMPDQVDDEVKKDRLIRLIELQNKIS
LEKSAELKGKVVEVLIEGVSKRDPNKLTSRTRTNKVVHFIGDESLIGKLANVKITETKAWTMQGELVEVLR

Sequences:

>Translated_471_residues
MRNSYYVSEEELKKQREIMEEIAWENRGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYKYTEDLEKADVLLFNTCAVRE
HAEVRVLGRVSQIKELKNRNPNLIIGISGCMMQEKHIVEAIREKYPHVDIVFGTHNIYKFPELLWQALNSRVQVIDVIEN
TQNVIEGLPIRRDSNLKAWVNIIYGCNNFCTYCIVPYTRGREKSRRPEDIIAEVKELAEKGYKEITLLGQNVNSYGKDLG
GDINFAKLLYMLNDIKGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKILKRMNRRYTREHYLEIIDKV
RANIPDIAITTDIIVGFPGETEEDFFQTLDLVERVRFDGAYTFMYSKRAGTPAASMPDQVDDEVKKDRLIRLIELQNKIS
LEKSAELKGKVVEVLIEGVSKRDPNKLTSRTRTNKVVHFIGDESLIGKLANVKITETKAWTMQGELVEVLR
>Mature_471_residues
MRNSYYVSEEELKKQREIMEEIAWENRGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYKYTEDLEKADVLLFNTCAVRE
HAEVRVLGRVSQIKELKNRNPNLIIGISGCMMQEKHIVEAIREKYPHVDIVFGTHNIYKFPELLWQALNSRVQVIDVIEN
TQNVIEGLPIRRDSNLKAWVNIIYGCNNFCTYCIVPYTRGREKSRRPEDIIAEVKELAEKGYKEITLLGQNVNSYGKDLG
GDINFAKLLYMLNDIKGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKILKRMNRRYTREHYLEIIDKV
RANIPDIAITTDIIVGFPGETEEDFFQTLDLVERVRFDGAYTFMYSKRAGTPAASMPDQVDDEVKKDRLIRLIELQNKIS
LEKSAELKGKVVEVLIEGVSKRDPNKLTSRTRTNKVVHFIGDESLIGKLANVKITETKAWTMQGELVEVLR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=36.3825363825364, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=36.3825363825364, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI93277076, Length=441, Percent_Identity=29.4784580498866, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI1786882, Length=437, Percent_Identity=43.9359267734554, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=28.7946428571429, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=475, Percent_Identity=34.5263157894737, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=293, Percent_Identity=30.0341296928328, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21356207, Length=506, Percent_Identity=36.3636363636364, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI19922432, Length=477, Percent_Identity=28.5115303983228, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_THETN (Q8RA72)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622974.1
- ProteinModelPortal:   Q8RA72
- SMR:   Q8RA72
- GeneID:   997766
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1356
- NMPDR:   fig|273068.3.peg.115
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14328
- BioCyc:   TTEN273068:TTE1356-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 54517; Mature: 54517

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSYYVSEEELKKQREIMEEIAWENRGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCH
YTEDLEKADVLLFNTCAVREHAEVRVLGRVSQIKELKNRNPNLIIGISGCMMQEKHIVEA
HHHHHHHCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHH
IREKYPHVDIVFGTHNIYKFPELLWQALNSRVQVIDVIENTQNVIEGLPIRRDSNLKAWV
HHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHH
NIIYGCNNFCTYCIVPYTRGREKSRRPEDIIAEVKELAEKGYKEITLLGQNVNSYGKDLG
HHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCC
GDINFAKLLYMLNDIKGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LKRMNRRYTREHYLEIIDKVRANIPDIAITTDIIVGFPGETEEDFFQTLDLVERVRFDGA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCE
YTFMYSKRAGTPAASMPDQVDDEVKKDRLIRLIELQNKISLEKSAELKGKVVEVLIEGVS
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
KRDPNKLTSRTRTNKVVHFIGDESLIGKLANVKITETKAWTMQGELVEVLR
CCCCHHHHHHHHCCCEEEEECCHHHHHHHHCEEEECCEEEEEHHHHHHHCC
>Mature Secondary Structure
MRNSYYVSEEELKKQREIMEEIAWENRGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCH
YTEDLEKADVLLFNTCAVREHAEVRVLGRVSQIKELKNRNPNLIIGISGCMMQEKHIVEA
HHHHHHHCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHH
IREKYPHVDIVFGTHNIYKFPELLWQALNSRVQVIDVIENTQNVIEGLPIRRDSNLKAWV
HHHHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHH
NIIYGCNNFCTYCIVPYTRGREKSRRPEDIIAEVKELAEKGYKEITLLGQNVNSYGKDLG
HHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCC
GDINFAKLLYMLNDIKGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LKRMNRRYTREHYLEIIDKVRANIPDIAITTDIIVGFPGETEEDFFQTLDLVERVRFDGA
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCE
YTFMYSKRAGTPAASMPDQVDDEVKKDRLIRLIELQNKISLEKSAELKGKVVEVLIEGVS
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
KRDPNKLTSRTRTNKVVHFIGDESLIGKLANVKITETKAWTMQGELVEVLR
CCCCHHHHHHHHCCCEEEEECCHHHHHHHHCEEEECCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336