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The map label for this gene is PcnB2 [H]

Identifier: 20807771

GI number: 20807771

Start: 1310089

End: 1311261

Strand: Direct

Name: PcnB2 [H]

Synonym: TTE1322

Alternate gene names: 20807771

Gene position: 1310089-1311261 (Clockwise)

Preceding gene: 20807770

Following gene: 20807772

Centisome position: 48.71

GC content: 33.33

Gene sequence:

>1173_bases
TTGCGCATACAAATTGAAGGCAAAGAATTGATCTCATATTTAAAAGAAATTTCACAAAAAGTAGGTGTAAAGTCATATAT
AGTTGGAGGGGTTGTGAGGGATTTTTTACTAGGGGTTGAAAACTGGGACATCGATGTAGTAGTAGAGGGAAATGGAATAG
ATTTTGCTTATGAGTTAAATGAATATATAAAGGGCGAATTGATAGAATATCCAGCTTTTAAAACTGCTACTATTAAAAAA
AGTGGCATTTCAATCGATGTAATTTCTGCCAGAAGAGAATATTACGATTATCCAGCAGCCTTGCCCAGGATTGAATTTGC
TGATATATATGAAGATATGAGAAGAAGAGACTTCACTATTAACACTTTGGCTTACGATGTGCTGGAGGAAAAGATACTTG
ACTATTTTGGGGGAATCGAAGATTTAAAAAAAGGGATAATAAGGGTTCTCCATGAAAAGAGCTTTATAGATGACCCCACG
AGGATATTTAGGGCTATAAGATATTCTGTGAGATATTCTTTTGAAATTGAAGCTAATACAGAGGCTTTGATGAAAGATTC
CATAAAAAATATTTCCCTCTTATCAGAAGACAGGATAAGGAATGAACTTTTTTTAATACTAAAAGAACCTAAAGCAAAGG
AAATGATAGAAAAAGTTATAGAATATGGGATTGATAAAGTAATCTTTAAAGATGTTCCTGTAAATGTAGATAAGCTAGAT
GTTTTTGTTGAAGATTTAGACTTAAGTTTATTCCGTCTTCTAATATTGTTTTATAAAATTGCTGAAAAAGACGTGGAGAA
AGTGCAGAAGGCTTTAAAGTTGAACAAAGAACATTTGAAAGCTCTTAGAGAACTGATTTTTCTAAGGGAAGAGATAAAAT
ACAGAAGGTGGATTAGAAAATTAAGAGAAACATTGGAAAGCGCTAGAAATGAAGTGCTGAAAGTTCTCGAAGTCATGGAA
GGAGATAAGGCGAAAAAAATAATTGAAAGCAAGCCTTATATAAAAGGAAAAGACATTAAAAACTTGGGAGTTTCACCTGG
CCCTTTATACGGAGAATTGCTGGATGAGGTATTTAAAGCTAAACTTGAAGGAAGACTGAAAAGCAAAGAAGAAGAGATTT
CGTTTGTAAAGAACATCTTACGAGTTGGAAAGGAGAATACAAGTTGCTGCTAG

Upstream 100 bases:

>100_bases
CTAAAACTAAGAAGTAGAGTCTTAGTTTTAGCTTTTTTTTATTTTAGGCTGTGATATAATATAAAGAGTAAGGGGAAATT
TAGGTAAAAAGGGTGGTTGT

Downstream 100 bases:

>100_bases
ATTTTATATACAGAATTCCAGCTTTGCTTTTGGCGATGAGTTTTCACGAGTTCAGCCATGGATATGTGGCTGACAGGCTA
GGAGACCCTACTCCGCGGCA

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRIQIEGKELISYLKEISQKVGVKSYIVGGVVRDFLLGVENWDIDVVVEGNGIDFAYELNEYIKGELIEYPAFKTATIKK
SGISIDVISARREYYDYPAALPRIEFADIYEDMRRRDFTINTLAYDVLEEKILDYFGGIEDLKKGIIRVLHEKSFIDDPT
RIFRAIRYSVRYSFEIEANTEALMKDSIKNISLLSEDRIRNELFLILKEPKAKEMIEKVIEYGIDKVIFKDVPVNVDKLD
VFVEDLDLSLFRLLILFYKIAEKDVEKVQKALKLNKEHLKALRELIFLREEIKYRRWIRKLRETLESARNEVLKVLEVME
GDKAKKIIESKPYIKGKDIKNLGVSPGPLYGELLDEVFKAKLEGRLKSKEEEISFVKNILRVGKENTSCC

Sequences:

>Translated_390_residues
MRIQIEGKELISYLKEISQKVGVKSYIVGGVVRDFLLGVENWDIDVVVEGNGIDFAYELNEYIKGELIEYPAFKTATIKK
SGISIDVISARREYYDYPAALPRIEFADIYEDMRRRDFTINTLAYDVLEEKILDYFGGIEDLKKGIIRVLHEKSFIDDPT
RIFRAIRYSVRYSFEIEANTEALMKDSIKNISLLSEDRIRNELFLILKEPKAKEMIEKVIEYGIDKVIFKDVPVNVDKLD
VFVEDLDLSLFRLLILFYKIAEKDVEKVQKALKLNKEHLKALRELIFLREEIKYRRWIRKLRETLESARNEVLKVLEVME
GDKAKKIIESKPYIKGKDIKNLGVSPGPLYGELLDEVFKAKLEGRLKSKEEEISFVKNILRVGKENTSCC
>Mature_390_residues
MRIQIEGKELISYLKEISQKVGVKSYIVGGVVRDFLLGVENWDIDVVVEGNGIDFAYELNEYIKGELIEYPAFKTATIKK
SGISIDVISARREYYDYPAALPRIEFADIYEDMRRRDFTINTLAYDVLEEKILDYFGGIEDLKKGIIRVLHEKSFIDDPT
RIFRAIRYSVRYSFEIEANTEALMKDSIKNISLLSEDRIRNELFLILKEPKAKEMIEKVIEYGIDKVIFKDVPVNVDKLD
VFVEDLDLSLFRLLILFYKIAEKDVEKVQKALKLNKEHLKALRELIFLREEIKYRRWIRKLRETLESARNEVLKVLEVME
GDKAKKIIESKPYIKGKDIKNLGVSPGPLYGELLDEVFKAKLEGRLKSKEEEISFVKNILRVGKENTSCC

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=385, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081691, Length=229, Percent_Identity=28.3842794759825, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=30.7017543859649, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=227, Percent_Identity=31.2775330396476, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45433; Mature: 45433

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQIEGKELISYLKEISQKVGVKSYIVGGVVRDFLLGVENWDIDVVVEGNGIDFAYELN
CEEEECHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHH
EYIKGELIEYPAFKTATIKKSGISIDVISARREYYDYPAALPRIEFADIYEDMRRRDFTI
HHHCCHHCCCCCCCEEEEECCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
NTLAYDVLEEKILDYFGGIEDLKKGIIRVLHEKSFIDDPTRIFRAIRYSVRYSFEIEANT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCH
EALMKDSIKNISLLSEDRIRNELFLILKEPKAKEMIEKVIEYGIDKVIFKDVPVNVDKLD
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHH
VFVEDLDLSLFRLLILFYKIAEKDVEKVQKALKLNKEHLKALRELIFLREEIKYRRWIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRETLESARNEVLKVLEVMEGDKAKKIIESKPYIKGKDIKNLGVSPGPLYGELLDEVFKA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KLEGRLKSKEEEISFVKNILRVGKENTSCC
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIQIEGKELISYLKEISQKVGVKSYIVGGVVRDFLLGVENWDIDVVVEGNGIDFAYELN
CEEEECHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHH
EYIKGELIEYPAFKTATIKKSGISIDVISARREYYDYPAALPRIEFADIYEDMRRRDFTI
HHHCCHHCCCCCCCEEEEECCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
NTLAYDVLEEKILDYFGGIEDLKKGIIRVLHEKSFIDDPTRIFRAIRYSVRYSFEIEANT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCH
EALMKDSIKNISLLSEDRIRNELFLILKEPKAKEMIEKVIEYGIDKVIFKDVPVNVDKLD
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHH
VFVEDLDLSLFRLLILFYKIAEKDVEKVQKALKLNKEHLKALRELIFLREEIKYRRWIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRETLESARNEVLKVLEVMEGDKAKKIIESKPYIKGKDIKNLGVSPGPLYGELLDEVFKA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KLEGRLKSKEEEISFVKNILRVGKENTSCC
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA